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Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course (open access)

Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course

Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr G.A.O. Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiment, essays,. preparation and analysis of plasmid DNA and various other topics. Part 2 covers the experiments conducted by Dr. Daniel Kunz and includes various topics like media preparation, phenotyping strains, conjugative transfer of plasmids, SDS-PAGE, induction and measurement of enzyme and transposon mutagenesis
Date: August 1998
Creator: Kumar, Shalini
System: The UNT Digital Library
Analysis of Phytoplankton Responses to Water Chemistry Dynamics in a Moderately Eutrophic North Texas Reservoir (open access)

Analysis of Phytoplankton Responses to Water Chemistry Dynamics in a Moderately Eutrophic North Texas Reservoir

Canonical correspondence analysis (CCA) was used to explore relationships between measured environmental variables and in situ phytoplankton communities in a moderately eutrophic North Texas Reservoir.
Date: May 1998
Creator: Banks, Kenneth E. (Kenneth Edward)
System: The UNT Digital Library
Callibaetis Floridanus (Ephemeroptera: Baetidae) Life History and Production in a West Texas Playa (open access)

Callibaetis Floridanus (Ephemeroptera: Baetidae) Life History and Production in a West Texas Playa

A life history study of Callibaetis floridanus was conducted over the wet cycle of a playa on the Southern High Plains of Texas from June through September 1995.
Date: May 1998
Creator: Anderson, Gregory (Gregory Mark)
System: The UNT Digital Library
Development and Application of an Assessment Protocol for Watershed Based Biomonitoring (open access)

Development and Application of an Assessment Protocol for Watershed Based Biomonitoring

With numerous bioassessment methodologies available, a regional protocol needs to be developed to ensure that results are comparable. A regional assessment protocol was developed that includes collecting five benthic macroinvertebrate samples, identifying organisms to genus, and calculating the following metrics: Number of Taxa, Total Number of Individuals, Simpson's Diversity Index, Shannon's Diversity Index, Percent Contribution of Dominant Taxa, Hilsenhoffs Biotic Index, and Percent Contribution of Dipterans. Once the protocol was developed, it was used to assess the Bayou Chico tributaries and watershed. All three tributaries had been significantly impacted by human activity as had the watershed as a whole. This study indicates that a regional protocol could be developed and is appropriate for biomonitoring at the watershed scale.
Date: May 1998
Creator: Schwartz, Joseph Howard
System: The UNT Digital Library
Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree (open access)

Development of an In Vitro Protoplast Culture System for Albizia Lebek (L.) Benth., an Economically Important Leguminous Tree

An in vitro system of generating protoplasts from their callus cultures was established. The friable callus was more productive in terms of producing protoplasts than the green compact callus. The concentration of the various cell wall degrading enzymes had an effect on the viability of the protoplasts in the medium. The protoplast system developed from the experiments was stable and could be used for the transformation experiments of Albizia lebek and for other plant improvement practices.
Date: August 1998
Creator: Sinha, Debleena
System: The UNT Digital Library
Estimated Extent and Fate of Chlorinated Solvent Contamination in the Soil of the Naval Air Station, Dallas, Texas (open access)

Estimated Extent and Fate of Chlorinated Solvent Contamination in the Soil of the Naval Air Station, Dallas, Texas

This thesis estimates the spatial extent of chlorinated solvent contamination of the soil at the Naval Air Station, Dallas, then estimates the fate and transport of these contaminants, over time, using the Soil Transport and Fate database and the Vadose-Zone Interactive Processes (VIP) modeling software. Geostatistical analysis identifies two areas with serious chlorinated solvent contamination. Fate and transport modeling estimates that this contamination will degrade and disperse from the soil phase to below regulatory limits within one year, although there is a risk of groundwater contamination. Contaminants are estimated to persist in the water and air phases of the soil. Further sampling is recommended to confirm the results of this study.
Date: August 1998
Creator: Trescott, Jill V. (Jill Virginia)
System: The UNT Digital Library
Life History and Case-building Behavior of Molanna Tryphena Betten (Trichoptera: Molannidae) in Two East Texas Spring-fed Streams (open access)

Life History and Case-building Behavior of Molanna Tryphena Betten (Trichoptera: Molannidae) in Two East Texas Spring-fed Streams

The life history and case-building behavior of Molanna tryphena from two spring-fed tributaries in East Texas were studied from January 1997 to May 1998.
Date: December 1998
Creator: Gupta, Tammi Spackman
System: The UNT Digital Library