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Site Directed Mutagenesis of Dienelactone Hydrolase
The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.
Date:
August 1995
Creator:
Al-Khatib, Haifa Yousef
System:
The UNT Digital Library