Chorine is used by the Village Creek Waste Water Treatment Plant to kill pathogenic microorganisms prior to discharge of the effluent into the Trinity River. The residual chlorine in the river impacted aquatic life prompting the U.S. Environmental Protection Agency in December 1990 to require dechlorination using sulfur dioxide. One pre-dechlorination and four post-dechlorination assessments of phytoplankton, periphyton, and zooplankton communities were conducted by the Institute of Applied Sciences at the University of North Texas. Dechlorination had no effect on the phytoplankton community. The periphyton community exhibited a shift in species abundance with a more even distribution of organisms among taxa. No change occurred in zooplankton species abundance, however, there was a decrease in zooplankton density following dechlorination.

Low oxygen tolerances of ten fishes were estimated using an original nitrogen cascade design, and reciprocally transformed to express responses as ventilated volume necessary to satisfy minimal oxygen demand (L·mg O2^-1). Values ranged from 0.52 to 5.64 L·mg^-1 and were partitioned into three statistically distinct groups. Eight stream fishes showed moderately high tolerances reflecting metabolic adaptations associated with stream intermittency. Juvenile longear sunfish and two mollies comprised the second group. High tolerance of hypoxia may allow juvenile sunfish to avoid predation, and mollies to survive harsh environmental oxygen regimens. The sheepshead minnow was the most tolerant species of low oxygen, of those examined, explaining its presence in severely hypoxic environments.