This report describes managerial aspects and briefly some technical accomplishments a Human Genome Database in Baltimore.

This progress report describes gains made in three projects entitled (1) 3-Dimensional nuclear topography of genes and chromosomes in interphase nuclei, (2) Sequence specific identification and perturbation of the genomic DNA in living cells by nonionic oligonucleotide analogs (Matagen), and Resolution and isolation of specific DNA restriction fragments.(DT)

This report describes managerial aspects and briefly some technical accomplishments a Human Genome Database in Baltimore.

The mechanisms by which hyperthermophilic archaebacteria grow and carry out metabolic functions at elevated temperatures have yet to be determined. The objective of this work is to develop an understanding of the metabolic characteristics of, and the electron transport enzymes involved in, hydrogen/sulfur transformation by hyperthermophilic archaebacteria. Efforts focus on the autotrophic H{sub 2}-oxidizing bacterium, Pyrodictium brockii which has an optimum growth temperature of 105{degrees}C. Biochemical and genetic characterization of enzymes involved in hydrogen oxidizing electron transport pathway. These including investigating the role of the membrane lipids in protecting the hydrogenase enzyme from thermal inactivation, characterization of a quinone and a c-type cytochrome, and analysis of the topology in the membrane in the net energy generating components are reported. The long-term goal is to understand some of the factors contributing to the biochemical basis of extreme thermophily.

Motion studies of the General Purpose Heat Source Module, GPHS, which were conducted in the heat pulse intervals associated with entries from earth gravity assist trajectories. The APL six-degree-of-freedom reentry program designated TMAGRA6C was used. The objectives of the studies were to (1) determine whether the GPHS module entering the earth`s atmosphere from an earth-gravity-assist trajectory has a preferred orientation during the heat pulse of reentry, (2) determine the effect of magnus force on the roll rate and angle of attack of the GPHS during an EGA entry, (3) determine the effect of the magnitude of pitch and roll damping on the GPHS motion.

Pamphlet from the Women Airforce Service Pilot 1992 reunion that describes the history of WASP and identifies people in several photographs that were meant to be viewed with the pamphlet.

The processes involved in the formation of mixed oxides powders were studied using the counterflow diffusion flame burner. Powders of different morphologies were obtained by varying the flame conditions (temperature, residence time) and the concentration ratio of the oxides precursors. In-situ particle size and number density were determined using dynamic light scattering and 90{degrees} light scattering. A thermophoretic sampling method and a larger scale powder collection device also was used to collect particles, and their size and morphology examined using transmission electron microscopy, X-ray diffraction and surface area measurement by gas absorption (BET). Our emphasis has been on TiO{sub 2}-SiO{sub 2}, SiO{sub 2}-GeO{sub 2} and Al{sub 2}O{sub 3}-TiO{sub 2}. The powders had a core-mantle-like (one oxide coated by the other) at low elevations in the burner and uniform mixture at higher elevations. They form chain-like structures in a low temperature flame and spherical particles in a higher temperature flame. Nanometer sized homogeneous particles of Aluminum Titanate could be obtained using Al(CH{sub 3}){sub 3} and TiCl{sub 4} as precursors both in a hydrogen fueled and a methane fueled counterflow diffusion flame burner, as well as in a hydrogen fueled parallel-flow diffusion flame burner.

Motion studies of the General Purpose Heat Source Module, GPHS, which were conducted in the heat pulse intervals associated with entries from earth gravity assist trajectories. The APL six-degree-of-freedom reentry program designated TMAGRA6C was used. The objectives of the studies were to (1) determine whether the GPHS module entering the earth's atmosphere from an earth-gravity-assist trajectory has a preferred orientation during the heat pulse of reentry, (2) determine the effect of magnus force on the roll rate and angle of attack of the GPHS during an EGA entry, (3) determine the effect of the magnitude of pitch and roll damping on the GPHS motion.

The mechanisms by which hyperthermophilic archaebacteria grow and carry out metabolic functions at elevated temperatures have yet to be determined. The objective of this work is to develop an understanding of the metabolic characteristics of, and the electron transport enzymes involved in, hydrogen/sulfur transformation by hyperthermophilic archaebacteria. Efforts focus on the autotrophic H{sub 2}-oxidizing bacterium, Pyrodictium brockii which has an optimum growth temperature of 105{degrees}C. Biochemical and genetic characterization of enzymes involved in hydrogen oxidizing electron transport pathway. These including investigating the role of the membrane lipids in protecting the hydrogenase enzyme from thermal inactivation, characterization of a quinone and a c-type cytochrome, and analysis of the topology in the membrane in the net energy generating components are reported. The long-term goal is to understand some of the factors contributing to the biochemical basis of extreme thermophily.

This progress report describes gains made in three projects entitled (1) 3-Dimensional nuclear topography of genes and chromosomes in interphase nuclei, (2) Sequence specific identification and perturbation of the genomic DNA in living cells by nonionic oligonucleotide analogs (Matagen), and Resolution and isolation of specific DNA restriction fragments.(DT)

Letter from Elaine Harmon to Rigdon Edwards discussing difficulty she is having in acquiring the photographs that he requested.

Letter from Elaine Harmon to Rigdon and Mary Emma wishing them a happy New Year, telling them about her pet dog, the conference in San Antonio, and military pilots.

Text for a speech about the responsibility of democracy, given by Barbara C. Jordan as a Williams Lecturer at Johns Hopkins University.