Cell division of ggeneflesmus quadricauda (Turp.) Breb. (Scenedesmaceae) is enhanced by methylglyoxal, a general inhibitor of cell division, at threshold concentration in conjunction with treatment timing related to growth stage of batch cultures. At 0.5 mM methylglyoxal concentration, cell division was significantly enhanced in algae treated in the logarithmic phase. Specific growth rates of methylglyoxal-treated cultures were rapidly increased at the beginning of logarithmic phase. Cultures inoculated with high cell numbers were less sensitive, but still showed high specific growth rates in logarithmic phase. Cell division in cultures which had low cell numbers was inhibited by 0.5 mM methylglyoxal treatment. Both specific activity of Glyoxalase I and the ratio of Glyoxalase I to Glyoxalase II of methylgloxal-treated cultures were higher than those of controls (1.3 and 2.1- fold, respectively). Pyruvate concentration in treated cultures was increased after methylglyoxal treatment.

Critics discussing Mark Twain's early skepticism have, to date, confined their explorations to short studies (articles or book chapters), brief references in passing, or buried their insights in discussions on other topics. Other critics ignore Twain's atheistic statements and see his beliefs as theistic or deterministic. Others ascribe his attitudes in the "dark writings" to late life disappointments. This study demonstrates that Twain's later attitudes towards religion, determinism, social reform and institutions were products of his family heritage, his social environment, and his early reading. Chapter 1 introduces the major premises of the study, and Chapter 2 reviews the critical background. Chapter 3 discusses the family and hometown influences: on Twain's skeptical thought, and Chapter A discusses Twain's early literary and philosophical influences. Chapter 5 examines Twain's early writings in letters and frontier tales and sketches, showing the development of his anti-religious attitudes. Chapter 6 concludes the study.