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Purification and Characterization of a Differentiation Factor From Rat Lung Conditioned Medium
A Differentiation Factor (DF) was purified from rat lung conditioned medium by a four-steps procedure. The DF has a molecular weight of 27000, and an isoelectric point of 4.70. Although DF is stable up to 60°C, it is sensitive to digestion by trypsin, chymotrypsin and subtilisin. DF forms granulocyte colonies in soft agar. Studies using anti-NRK CSF antibody demonstrated that DF is distinct from GM-CSF.
Date:
May 1988
Creator:
Ansari, Naser A. (Naser Awni)
System:
The UNT Digital Library