A unique undosciribed gramnegative rod is extensively characterized in this study. The cells of this unusual water isolate measure 1.2 X 6.5 microns, The most distinguishing characteristic of the bacterium is a polar tuft of 35-40 flagella that aggregate to function as a single organelle which is visible under phase contrast. Aging cells deposit poly- -hydroxybutyric acid granules which are bound by an inclusion membrane made up of four distinct layers. It also possesses an unusual exterior membrane outside the cell wall which contains large fibrils of protein running at a slight angle to the longitudinal axis of the cell. The guanosine-cytosine ratio was found to be 62.2$. The organism's taxonomic position was further investigated by immunological, morphological, and biochemical methods. It was found to be most closely akin to members of the genus Pseudo onas, although somewhat divergent from other species classified in this genus. After careful evaluation of the findings obtained during this study, the new bacterium was subsequently named Pseudomonas multiflagella.

Distributional data and taxonomic keys art presented for thirty-four species of Plecoptera known to occur in Oklahoma. Ten species are new records for the state. Descriptions are provided for two species new to science, Zealeuctra cherokee and Isoperla brevis, and for the previously unknown male of Strophopteryx cucullata Frison and female of Helopicus nalatus (Frison).

The objective of this investigation was to provide new and more detailed information on the life history and ecology of Neoperla clymene, through an intensive study of the species in the Brazos River, Texas.

The purpose of this study was twofold. The first was to isolate, purify, and characterize the deoxyribonucleic acid (DNA) of Azotobacter vinelandii ATCO 12837. The second was to determine if there was irreversible binding of homologous 32P labeled DNA to recipient A. vinelandii cells.

The effects of thermal discharges on the density, distribution, production, and drift of benthic organisms were studied at North Lake reservoir.

The coexistence of Dipodomys ordii and Perognathus hispidus with Peromyscus maniculatus and Reithrodontomys montanus was studied in a grassland association of central Texas. The food habits of these species were compared with information from habitat vegetation analysis in an effort to determine food selectivity and the amount and importance of niche overlap and competition among these rodents.

This study was initiated after finding two chromosomal types of Peromyscus maniculatus north and south of the Red River in Texas and Oklahoma. The problem was to explain the chromosomal variations and their implications to the systematics of the grassland subspecies of P. maniculatus in this region.

Annual lipid cycles were determined for adult male and female Cnemidophorus tigris collected near El Paso, Texas during 1970-1971.

Low-frequency ultrasonic energy was utilized in an attempt to induce visible mutations in the parasitoid wasp Mormoniella vitripennis. This study demonstrates that low frequency ultrasound may be used as an effective mutagenic agent in this organism, and suggests that it may have applications to other genetic systems.

This investigation was concerned with the relationship of the slime layer material of Pseudomonas aeruginosa, Verder and Evans strain 1369, to the presumably somatic "O" type of antigen used by these authors as the base for their serological schema.

The purpose of this research was to attempt to duplicate the in vitro bactericidal test against Pseudomonas aeruginosa as reported by Bjornson and Michael and to correlate agglutinating titer of an antiserum to protective activity.

The purpose of this study was to determine the effect of varying concentrations of geosmin on the growth of Bacillus cereus.

Aldolase from human heart has been purified 128-fold to a final specific activity of 11.52 units per mg. The purification procedure employed column chromatography on phosphocellulose.

An efficient purification procedure of Ascaris suum muscle utilizing ion exchange column chromatography has been developed.

This study was undertaken in order to provide further insight into the effects of artificial heating on the fisheries of a small reservoir in the Southwest. The following specific objectives were established: (1) to map the reservoir for the distribution of heated water, (2) to determine the distribution of selected species of sports, rough, and forage fish in areas affected by the effluent and in areas not affected, (3) to compare size and condition of selected species of fish from areas affected by the effluent to size and condition of fish from areas not affected, and (4) to compare food habits of channel catfish in areas affected by the effluent to the food habits of channel catfish in areas not affected.

In view of its varied concepts and interpretations, and because of the discrepancies produced by the previous utilization of polydispersed dextrans, a study using extremely narrow-range molecular weight dextran fractions was initiated to reevaluate and consolidate some of the aspects of capillary permeability. A portion of the study was performed under decreased barometric pressure in order to clarify further some of the mechanisms involved in particulate transfer across the capillary endothelial membranes. Gel filtration procedures augmented the study as an assessment of the polydispersity effects of the dextrans employed.

The purpose of the investigation was to locate the plant population which had the greatest impact on soil nitrogen in a successional sequence from newly deposited alluvia to a mature streamside forest, and to evaluate the pioneer populations in terms of their annual nitrogen contribution.

Two isoenzymes of malate dehydrogenase were isolated and partially purified from the midge larva Glyptotendipes barbipes. Differential centrifugation followed by cellulose acetate and polyacrylamide gel electrophoresis revealed one isoenzyme associated with the mitochondrial fraction and another form found only in the cytoplasm.

The potential growth-regulating compound 4-chloroglutaranilic acid (CGA) was tested in whole-plant bioassay systems which utilized sunflower seedlings (Helianthus annuus, L.). Test systems included the growth of plants in soil , solid inert (Vermiculite) substrate, and hydroponic (Seed-Pak) pouches.