In the class of the polycondensed aromatic hydrocarbons, the concept of localized charge in a {sigma}-complex, e.g., the carbonium ion formed by addition of a proton or other electrophile to an aromatic ring, has so far received experimental evidence only in the case of anthracene. The ultraviolet spectrum of this compound in sulfuric acid is similar to that of the benzhydryl cation. Ph{sub c}C{sup +}Me. This result implies the protonation of anthracene at one meso-carbon atom and the localization of the positive charge at the other. The {sigma}-complex of the potent carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) in acidic medium offers another example of this type of charge localization. The determination of structure was achieved by comparing the NMR spectra of DMBA in neutral and acidic solvents. The ratio of the integrated intensities of the proton peaks in the spectrum of DMBA is, from right to left, 3:3:5:1:1:2:1. The assignment of the 7-CH{sub 3} ({sigma}3.00) and 12-CH{sub 3} ({sigma}3.29) resonances was made by comparison with the spectra of 7-methylbenz[a]anthracene (7-CH{sub 3}, {sigma}3.03) and 12-methylbenz[a]anthracene (12-CH{sub 3}, {sigma}3.32) under the same conditions. A good general correlation exists between the methyl chemical shifts of the twelve monosubstituted methylbenzanthrancenes and the corresponding aryl protons of benzanthracene.

The mechanism of action of p-hydroxybenzoate hydroxylase from Pseudomonas putida, strain M-6, has been investigated. The aromatic substrate analogues, benzoate, p-fluorobenzoate, p-chlorobenzoate, p-nitrobenzoate, p-aminobenzoate, and 6-hydroxynicotinate, are found to be competitive inhibitors. This finding differs from the previously reported noncompetitive behavior in a different buffer system. The optical activity of the enzyme-inhibitor complex is studied. From the kinetic and circular dichroism (CD) measurements, they have found that the carboxyl moiety is necessary and sufficient for the enzyme-substrate binding, whereas the hydroxyl group alone will not lead to binding. There are two classes of inhibitory analogues: one causes changes in CD spectra of the enzyme similar to those evoked by the substrate, and the other does not cause significant changes. the results indicate that more than one mode of enzyme-inhibitor interaction is involved. The CD of the enzyme-NADPH complex under anaerobic conditions suggests that the oxidized enzyme and reduced pyridine nucleotide form a complex, both in the absence and presence of the substrate, p-hydroxybenzoate. Furthermore, evidence for a ternary complex is given.

The discussion is presented under the following chapter titles: geothermal resource investigations, Imperial Valley, California; the source of geothermal heat; status of geothermal resources (worldwide); geothermal aspects of Imperial Valley, California; potential geothermal development in Imperial Valley; environmental considerations; and proposed plan for development. (JGB)

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We propose to study K{sup -}p and {bar p}p interactions at 200 GeV in the hydrogen filled NAL 30'' bubble chamber with a supplementary visual spectrometer. We request 200,000 pictures. This exposure is intended as a follow up experiment to our proposal ''Multiparticle {pi}{sup -}p Interactions at 130 GeV and at the Highest Energy'' of April 23, 1971.

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We wish to search for new neutral particles by investigating the composition of a wide angle neutral beam produced by high energy protons on a Be target. Photons in the beam will be removed by a Pb converter. The resultant neutral beam will be incident on the 30 inch bubble chamber. The composition of the beam will be determined from the kinematic analysis of selected interactions in the bubble chamber, through the decays of neutral particles and through possible anomalies in the hydrogen interactions. As a byproduct we will measure the K-Long flux through the process K-Long + P {yields} K-Short + all. By a slight change in the geometry we can look at a region immediately downstream of the target and measure the characteristics of neutral particles arising from the decay of hyperons and other particles produced in the Be target. We do not require any external equipment besides beam monitoring equipment. We request an initial exposure of 20,000 pictures.

Two designs of thin annulus neptunium target elements, fabricated to produce medical grade Pu-238 in the Hanford production reactors and to be problem-free in separations and waste disposal operations, had successful in-reactor performances. Results of the BNW separations are not yet available. Examination of five N Reactor ball channels revealed no graphite breakage. The separations which were evident in only two channels were not great enough to result in ball losses after a ball drop. KE Reactor deactivation activities to affect a standby condition continued. N Reactor deactivation activities have been limited to criteria and procedure development and the scoping of necessary capital work. No irreversible physical changes have been made.

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