Super-resolution 3D imaging was achieved using newly synthesized photoactivatable quantum dot (PAQ dot) probes. Quantum dots were modified with a novel quencher system to make them photoactivatable. The unique properties of these PAQ dots enable single-fluorophore localization in three dimensions using a confocal microscopy optical sectioning method. Myosin and tropomyosin of rabbit myofibrilar bundles were specifically labeled with the newly synthesized PAQ dot. A sufficient number of single quantum dots were photoactivated, localized and reduced to their centroid and then reconstructed to a super-resolution image. The acquired super-resolution image shows a lateral and an axial sub-diffraction resolution and demonstrates ultrafine striations with widths less than 70 nm that are not evident by conventional confocal microscopy. The striations appear to be related to nebulin thin filament binding protein. This newly developed imaging system is cutting edge for its high resolution and localization as well its simplicity and convenience.

A dearth of pre-existing research in the field prompted this thesis on whether traditional econometric analyses of war deterrent alliances are applicable to modern alliances for counter terror purposes. Apparent foundational and contextual differences between the two types of alliances and the costs and benefits member nations derive from each lead the author to theorize that factors contributing to the formation of each alliance are fundamentally similar. Multiple types of statistical models are used to measure variables from the Correlates of War and Polity datasets combined with custom variables in a new dataset concerning major transnational terrorist attacks and the resultant alliances in testing the effect of traditionally contributing formation factors on alliances against terrorism. The results indicate that some contributing factors are similar, extant analysis tools have utility and that further investigation is justified.

Preparative mass spectrometry is an important method for the synthesis of new materials. Recently, soft landing mass spectrometry has been used to land ions on surfaces to coat or otherwise alter them. Commercial soft landing instruments do not yet exist, and the physical phenomenon of soft landing has not yet been fully described. For future ion mobility soft landing research, the theory of ion mobility, ion optics and soft landing is discussed, and 2 soft landing instruments have been built and described, along with proof of concept experiments for both instruments. Simulations of the process of ion mobility and ion optics for use in these instruments, as well as some preliminary results for the optics are included. Surfaces described include copper on mica and iron on silicon. Self assembly of soft landed ions is observed on the surfaces. The instruments constructed will be useful for future soft landing research, and soft landing can be used for future materials research with special attention focused on the self-assembly of the landed ions.

The preface, "Against Buses: Charles Baxter and the Contemporary Epiphany" deals with the epiphany as a potential ending to short stories. Baxter holds that epiphanies are trite and without purpose in today's fiction. I argue that Baxter's view, while not without merit, is limiting. Beginning with James Joyce and Katherine Anne Porter and moving to my own work, I discuss how some epiphanies, particularly false ones, can enhance rather than detract from excellent fiction. Five short stories make up the remainder of this thesis: "Dedication," "Taking it with You," "Transition to Flowers," "Profile in Courage," and "Henderson Street Bazaar."

This study investigated the effect of implementing high-probability request sequences prior to the delivery of instructions to transition in a child with severe mental retardation. Data were collected on latency to comply with a low-probability request to transition and a modified version of the low-probability request. Implementation of high-probability request sequences resulted in shortened latencies to comply with the modified low-probability request instructing the child to engage in a preferred activity located at the endpoint of the transition.

Smart phones today have become increasingly popular with the general public for its diverse abilities like navigation, social networking, and multimedia facilities to name a few. These phones are equipped with high end processors, high resolution cameras, built-in sensors like accelerometer, orientation-sensor, light-sensor, and much more. According to comScore survey, 25.3% of US adults use smart phones in their daily lives. Motivated by the capability of smart phones and their extensive usage, I focused on utilizing them for bio-medical applications. In this thesis, I present a new application for a smart phone to quantify the vital signs such as heart rate, respiratory rate and blood pressure with the help of its built-in sensors. Using the camera and a microphone, I have shown how the blood pressure and heart rate can be determined for a subject. People sometimes encounter minor situations like fainting or fatal accidents like car crash at unexpected times and places. It would be useful to have a device which can measure all vital signs in such an event. The second part of this thesis demonstrates a new mode of communication for next generation 9-1-1 calls. In this new architecture, the call-taker will be able to control the …

Cyanide is a well known toxicant that arises in the environment from both biological and industrial sources. Bacteria have evolved novel coping mechanisms for cyanide and function as principal agents in the biosphere for cyanide recycling. Some bacteria exhibit the unusual ability of growing on cyanide as the sole nitrogen source. One such organism is Pseudomonas fluorescens NCIMB 11764 (Pf11764) which employs a novel oxidative mechanism for detoxifying and assimilating cyanide. A unique complex of enzymes referred to as cyanide oxygenase (CNO) is responsible for this ability converting cyanide to ammonia which is then assimilated. Because one component of the four member CNO complex was previously shown to act on cyanide independent of the other members, its characterization was sought as a means of gaining a better understanding of the overall catalytic mechanism of the complex. Preliminary studies suggested that the enzyme belonged to a subset of nitrilase enzymes known as cyanide dihydratases (CynD), however, a cynD-like gene in Pf11764 could not be detected by PCR. Instead, a separate nitrilase (Nit) linked to cyanide metabolism was detected. The corresponding nit gene was shown to be one of a conserved set of nit genes traced to a unique cluster in bacteria …

Keyphrases describe a document in a coherent and simple way, giving the prospective reader a way to quickly determine whether the document satisfies their information needs. The pervasion of huge amount of information on Web, with only a small amount of documents have keyphrases extracted, there is a definite need to discover automatic keyphrase extraction systems. Typically, a document written by human develops around one or more general concepts or sub-concepts. These concepts or sub-concepts should be structured and semantically related with each other, so that they can form the meaningful representation of a document. Considering the fact, the phrases or concepts in a document are related to each other, a new approach for keyphrase extraction is introduced that exploits the semantic relations in the document. For measuring the semantic relations between concepts or sub-concepts in the document, I present a comprehensive study aimed at using collaboratively constructed semantic resources like Wikipedia and its link structure. In particular, I introduce a graph-based keyphrase extraction system that exploits the semantic relations in the document and features such as term frequency. I evaluated the proposed system using novel measures and the results obtained compare favorably with previously published results on established benchmarks.

Although Magdeburg cantor Heinrich Grimm was frequently listed among prominent musical figures of the early seventeenth century such as Heinrich Schütz, Johann Hermann Schein, and Michael Praetorius in music lexica through the nineteenth century, he has almost disappeared from modern scholarship. However, a resurgence in Grimm studies has begun in recent years, especially in the areas of biographical study and compositional output. In this study, I examine the yet unexplored music-analytic perspective by investigating the stylistic characteristics of Grimm's 1631 motet, Unser Leben wehret siebenzig Jahr'. Furthermore, I compare his compositional technique to that of his contemporaries and predecessors with the goal of examining the work from both Renaissance and Baroque perspectives.

This study seeks to use a dual-theoretical approach, through the use of para-social relationship theory and economic data analysis, to explain the success of reality television since the early 2000s. This study uses both qualitative and quantitative components to understand the growth of reality television. This study includes a literature analysis of both methodologies used. Focus groups were used to seek to find a strong level of para-social interaction in viewers of reality television. Two focus groups were conducted with participants 18-35. There were a total of 16 participants who attended the focus group sessions. The information collected suggested that viewers of reality television formed para-social relationships. It appeared that female viewers were more likely to form para-social relationships than male viewers.

About 8.3% of individuals diagnosed with diabetes mellitus (DM) are diagnosed with comorbid depression, a higher rate than the general adult population. This project examined the differences of depression symptoms experienced between diabetic and matched non-diabetic individuals and the relationship of daily activity and nutrition behaviors with depression between these groups. The 2005-2006 National Health and Nutrition Examination Survey (NHANES) was utilized to assess: depression symptoms, diabetic glycemic control as measured by glycoginated hemoglobin (HbA1c), amount of physical activity, percentage of macronutrients, daily frequencies of foods consumed, and the use of nutritional food labels to make food choices. A sample of diabetic (n = 451) and non-diabetic individuals (n = 451) were matched to on age, gender, ethnicity, and education. The diabetic individuals experienced greater depression on both continuous and ordinal diagnostic variables. Counter to expectation, there was no relationship observed between depression and HbA1c in diabetic individuals, r = .04, p > .05.

About 8.3% of individuals diagnosed with diabetes mellitus (DM) are diagnosed with comorbid depression, a higher rate than the general adult population. This project examined the differences of depression symptoms experienced between diabetic and matched non-diabetic individuals and the relationship of daily activity and nutrition behaviors with depression between these groups. The 2005-2006 National Health and Nutrition Examination Survey (NHANES) was utilized to assess: depression symptoms, diabetic glycemic control as measured by glycoginated hemoglobin (HbA1c), amount of physical activity, percentage of macronutrients, daily frequencies of foods consumed, and the use of nutritional food labels to make food choices. A sample of diabetic (n = 451) and non-diabetic individuals (n = 451) were matched to on age, gender, ethnicity, and education. The diabetic individuals experienced greater depression on both continuous and ordinal diagnostic variables. Counter to expectation, there was no relationship observed between depression and HbA1c in diabetic individuals, r = .04, p > .05.

This thesis examines the aesthetic of ballets adapted for BBC Television by producer Margaret Dale, beginning with her entrance to the BBC's training program in 1955 and culminating with her commissioned work Houseparty, which aired in 1964. A historical and organizational framework is discussed regarding the BBC's cultural mission and view of arts programming, as well as general developmental milestones in programming contextualizing Dale's working conditions. Particular focus is placed upon the appropriation of Romantic narrative ballets and their significance in reinforcing an aristocratic and culturally divisive structure in the arts. Textual analyses consider issues of restaging, camera placement, and lighting, as well as television's intimacy and relationship to characterization in ballet narratives.

Despite high levels of exposure, firefighter posttraumatic stress disorder (PTSD) rates are unclear. Likewise, questions remain regarding how social interactions and beliefs about emotion might interact to influence PTSD in firefighters. In this study, U.S. urban firefighters (N = 225) completed measures of social support, negative social interactions, and fear of emotion which were then used via regression analyses to predict PTSD symptoms. Each independent variable predicted PTSD beyond variance accounted for by demographic variables. Additionally, fear of emotion emerged as the strongest individual predictor of PTSD and a moderator of the relation between social interactions and PTSD symptoms. These findings emphasize the importance of beliefs about emotion; both in how these beliefs might influence the expression of PTSD symptoms, and in how the social networks of trauma survivors might buffer distress.

The purpose of this study was to determine the effects of psychographic (shopping orientation, lifestyle, social class), demographic (gender, ethnicity, age), and geographic (area of residence) variables on time-related shopping behaviors when shopping for clothing for the self. The concept of time-related shopping behaviors has not been the focus of any study of the American market. Data (N = 550) were collected via a questionnaire with an online survey company. Through analysis of chi square statistics, ANOVA, Pearson product-moment correlation, and factor analysis, it was found that psychographics and demographics affected time-related and other shopping behaviors. Geographics was found to affect shopping behavior, but not specifically the time-related shopping behaviors studied.

The guitar chord (a sonority based on the open strings of the guitar) is one of Alberto Ginastera's compositional trademarks. The use of the guitar chord expands throughout forty years, creating a common link between different compositional stages and techniques. Chapters I and II provide the historical and technical background on Ginastera's life, oeuvre and scholar research. Chapter IV explores the origins of the guitar chord and compares it to similar specific sonorities used by different composers to express extra-musical ideas. Chapter V discusses Ginastera's initial uses and modifications of the guitar chord. Chapter VI explores the use of the guitar chord as a referential sonority based on Variaciones Concertantes, Op. 23: I-II, examining vertical (subsets) and horizontal (derivation of motives) aspects. Chapter VII explores uses of trichords and hexachords derived from the guitar chord in the Sonata for Guitar Op. 47.

The accurate determination of malingering relies on the use of validated and clinically relevant assessment measures. Simulation design is the most often-used research design to accomplish this. However, its external validity is sometimes questioned. The goal of the thesis was to systematically evaluate these major elements: situation, incentives, and coaching. The situation in simulation studies can vary from relevant (academic failure in a college population) to irrelevant (capital murder) for the samples being studied. Incentives refer to the external motivation given to improve simulators' performance and can be positive (extra credit and monetary reward) or negative (extra time and effort). Finally, coaching refers to whether the participant receives any information on detection strategies that are designed to identify feigners. Using a large undergraduate sample in a factorial design, results indicate that a scenario familiar to the participants generally improved the believability of their responses. Coaching also improved the ability to feign convincingly, while incentive type was not associated with any change in scores. The implications of these findings for future research designs and the connection to practice are discussed.

Ni/TiC metal matrix composites have been processed using the laser engineered net shaping (LENS) process. As nickel does not form an equilibrium carbide phase, addition of a strong carbide former in the form of titanium reinforces the nickel matrix resulting in a promising hybrid material for both surface engineering as well as high temperature structural applications. Changing the relative amounts of titanium and carbon in the nickel matrix, relatively low volume fraction of refined homogeneously distributed carbide precipitates, formation of in-situ carbide precipitates and the microstructural changes are investigated. The composites have been characterized in detail using x-ray diffraction, scanning electron microscopy (including energy dispersive spectroscopy (XEDS) mapping and electron backscatter diffraction (EBSD)), Auger electron spectroscopy, and transmission (including high resolution) electron microscopy. Both primary and eutectic titanium carbides, observed in this composite, exhibited the fcc-TiC structure (NaCl-type). Details of the orientation relationship between Ni and TiC have been studied using SEM-EBSD and high resolution TEM. The results of micro-hardness and tribology tests indicate that these composites have a relatively high hardness and a steady-state friction coefficient of ~0.5, both of which are improvements in comparison to LENS deposited pure Ni.

This thesis deals with the decentralized autonomous system, in which individual nodes acting like peers, communicate and participate in collaborative tasks and decision making processes. An experimental test-bed is created using four Garcia robots. The robots act like peers and interact with each other using user datagram protocol (UDP) messages. Each robot continuously monitors for messages coming from other robots and respond accordingly. Each robot broadcasts its location to all the other robots within its vicinity. Robots do not have built-in global positioning system (GPS). So, an indoor localization method based on signal strength is developed to estimate robot's position. The signal strength that the robot gets from the nearby wireless access points is used to calculate the robot's position. Trilateration and fingerprint are some of the indoor localization methods used for this purpose. The communication functionality of the decentralized system has been tested and verified in the autonomous systems laboratory.

This study of the variable use of ne in synchronous electronic French discourse follows the methodological guidelines and the theoretical framework proposed and subsequently elaborated by Labov for analyzing variable features of language. This thesis provides a quantitative variable rule (i.e., VARBRUL) analysis including age as a factor group (i.e., independent variable), thereby making a new contribution to this area of inquiry. The data (50,000 words from the vingtaine 'twentysomething' channel and 50,000 words from the cinquantaine 'fiftysomething' channel) are a subset of 100,000 words from a corpus of one million words collected in 2008 by the thesis director from the public chat server EuropNet. This study aims to answer the following overarching question: To what extent does age-compared to other factors-influence the variable use of ne in verbal negation in synchronous electronic French discourse? In order to answer this question, and possibly others, the VARBRUL analysis will include age, subject (e.g., noun vs. pronoun), type of second negative particle (e.g., pas 'not', jamais 'never', personne 'no one'/'nobody', and so forth), as well as verbal mood/tense.

This descriptive study uses a qualitative, content analysis to examine the middle school visual arts and core Texas Essential Knowledge and Skills (TEKS) to determine the potential common learning activities that can be aligned between the two. By performing an alignment of the potential common learning activities present in the middle school visual art TEKS and the middle school core TEKS, I demonstrate that there is a foundation for curriculum integration in the Texas middle school visual arts classroom.

The International Criminal Tribunal for the Former Yugoslavia (ICTY) has been both contentious and successful. By examining the ICTY from a Levinasian ethical standpoint, we might be able to understand how the court uses language to enforce ethical and moral standards upon post-war societies. Using linguistic methods of analysis combined with traditional data about the ICTY, I empirically examine the court using ordinary least squares (OLS) in order to show the impact that language has upon the court's decision making process. I hypothesize that the court is an ethical entity, and therefore we should not see any evidence of bias against Serbs and that language will provide a robust view of the court as an ethical mechanism.

This study investigated how two college newspapers cover race and how the papers employed racial stereotypes when describing sources. One of newspapers is a student-produced paper at a private university. The other is a student-produced newspaper at a public university. The study conducted content analyses of front-page news stories in both college newspapers. The sources in the story were analyzed for racial stereotypes. Stereotypes were identified based on frames used in modern racism research. A t-test and chi-square were used to compare the coverage of minorities to Whites. Once the quantitative content analysis was completed, I used textual analysis to identify what ways the news stories used stereotypical coverage of minorities. The study used critical media theory.

Previous research suggests there are a number of variables that are associated with the expression of schizotypal personality disorder (SPD) symptoms. Such variables include childhood trauma, depression and anxiety, substance use, normal-range personality traits, ethnicity, and gender. However, research to date has not examined all of these variables in a single study to determine how they may be interrelated or differentially related to SPD symptom domains. Of particular interest is the association of these variables as explained by the diathesis-stress model. This study utilized a convenience sample of 298 undergraduate students to examine a continuous range of scores for symptoms of SPD and how the interrelation of biological factors such as gender and ethnicity and psychosocial factors and stressors such as childhood trauma and personality traits, specifically neuroticism and extroversion, influence the expression of SPD symptoms. It was predicted that anxiety, depression, stress, and childhood trauma would positively correlate to SPD symptoms. It was also hypothesized that neuroticism and substance use would positively correlate to schizotypal traits and extroversion would be negatively correlated to schizotypal traits as measured by the Schizotypal Personality Questionnaire-Brief. It was further hypothesized that psychosocial stressors would be moderated by the aforementioned biological factors.