Fast Ignition Experimental and Theoretical Studies (open access)

Fast Ignition Experimental and Theoretical Studies

We are becoming dependent on energy more today than we were a century ago, and with increasing world population and booming economies, sooner or later our energy sources will be exhausted. Moreover, our economy and welfare strongly depends on foreign oil and in the shadow of political uncertainties, there is an urgent need for a reliable, safe, and cheap energy source. Thermonuclear fusion, if achieved, is that source of energy which not only will satisfy our demand for today but also for centuries to come. Today, there are two major approaches to achieve fusion: magnetic confinement fusion (MFE) and inertial confinement fusion (ICF). This dissertation explores the inertial confinement fusion using the fast ignition concept. Unlike the conventional approach where the same laser is used for compression and ignition, in fast ignition separate laser beams are used. This dissertation addresses three very important topics to fast ignition inertial confinement fusion. These are laser-to-electron coupling efficiency, laser-generated electron beam transport, and the associated isochoric heating. First, an integrated fast ignition experiment is carried out with 0.9 kJ of energy in the compression beam and 70 J in the ignition beam. Measurements of absolute K{sub {alpha}} yield from the imploded core revealed …
Date: October 20, 2006
Creator: Akli, K
System: The UNT Digital Library
A RANS/DES Numerical Procedure for Axisymmetric Flows with and without Strong Rotation (open access)

A RANS/DES Numerical Procedure for Axisymmetric Flows with and without Strong Rotation

A RANS/DES numerical procedure with an extended Lax-Wendroff control-volume scheme and turbulence model is described for the accurate simulation of internal/external axisymmetric flow with and without strong rotation. This new procedure is an extension, from Cartesian to cylindrical coordinates, of (1) a second order accurate multi-grid, control-volume integration scheme, and (2) a k-{omega} turbulence model. This paper outlines both the axisymmetric corrections to the mentioned numerical schemes and the developments of techniques pertaining to numerical dissipation, multi-block connectivity, parallelization, etc. Furthermore, analytical and experimental case studies are presented to demonstrate accuracy and computational efficiency. Notes are also made toward numerical stability of highly rotational flows.
Date: October 30, 2007
Creator: Andrade, A J
System: The UNT Digital Library
Soft X-ray emission spectroscopy of liquids and lithium batterymaterials (open access)

Soft X-ray emission spectroscopy of liquids and lithium batterymaterials

Lithium ion insertion into electrode materials is commonly used in rechargeable battery technology. The insertion implies changes in both the crystal structure and the electronic structure of the electrode material. Side-reactions may occur on the surface of the electrode which is exposed to the electrolyte and form a solid electrolyte interface (SEI). The understanding of these processes is of great importance for improving battery performance. The chemical and physical properties of water and alcohols are complicated by the presence of strong hydrogen bonding. Various experimental techniques have been used to study geometrical structures and different models have been proposed to view the details of how these liquids are geometrically organized by hydrogen bonding. However, very little is known about the electronic structure of these liquids, mainly due to the lack of suitable experimental tools. In this thesis examples of studies of lithium battery electrodes and liquid systems using soft x-ray emission spectroscopy will be presented. Monochromatized synchrotron radiation has been used to accomplish selective excitation, in terms of energy and polarization. The electronic structure of graphite electrodes has been studied, before and after lithium intercalation. Changes in the electronic structure upon lithiation due to transfer of electrons into the graphite …
Date: October 27, 2004
Creator: Augustsson, Andreas
System: The UNT Digital Library
Measurement of the top-antitop quark pair differential cross section with respect to the invariant mass of the pair in proton-antiproton collisions at a center of mass energy of 1.96 TeV (open access)

Measurement of the top-antitop quark pair differential cross section with respect to the invariant mass of the pair in proton-antiproton collisions at a center of mass energy of 1.96 TeV

I present a measurement of the t{bar t} differential cross section, d{sigma}/dM{sub t{bar t}}, in p{bar p} collisions at {radical}s = 1.96 TeV using 2.7 fb{sup -1} of CDF II data. I find that d{sigma}/dM{sub t{bar t}} is consistent with the Standard Model expectation, as modeled by PYTHIA with CTEQ5L parton distribution functions. I set limits on the ratio {kappa}/M{sub Pl} in the Randall-Sundrum model by looking for Kaluza Klein gravitons which decay to top quarks. I find {kappa}/M{sub Pl} > 0.16 at the 95% confidence level.
Date: October 1, 2008
Creator: Bridgeman, Alice
System: The UNT Digital Library
Coherence techniques at extreme ultraviolet wavelengths (open access)

Coherence techniques at extreme ultraviolet wavelengths

The renaissance of Extreme Ultraviolet (EUV) and soft x-ray (SXR) optics in recent years is mainly driven by the desire of printing and observing ever smaller features, as in lithography and microscopy. This attribute is complemented by the unique opportunity for element specific identification presented by the large number of atomic resonances, essentially for all materials in this range of photon energies. Together, these have driven the need for new short-wavelength radiation sources (e.g. third generation synchrotron radiation facilities), and novel optical components, that in turn permit new research in areas that have not yet been fully explored. This dissertation is directed towards advancing this new field by contributing to the characterization of spatial coherence properties of undulator radiation and, for the first time, introducing Fourier optical elements to this short-wavelength spectral region. The first experiment in this dissertation uses the Thompson-Wolf two-pinhole method to characterize the spatial coherence properties of the undulator radiation at Beamline 12 of the Advanced Light Source. High spatial coherence EUV radiation is demonstrated with appropriate spatial filtering. The effects of small vertical source size and beamline apertures are observed. The difference in the measured horizontal and vertical coherence profile evokes further theoretical studies on …
Date: October 1, 2002
Creator: Chang, Chang
System: The UNT Digital Library
Radiative Leptonic B Decays (open access)

Radiative Leptonic B Decays

We present the results of a search for B{sup +} meson decays into {gamma}{ell}{sup +}{nu}{sub {ell}}, where {ell} = e,{mu}. We use a sample of 232 million B{bar B} meson pairs recorded at the {Upsilon}(4S) resonance with the BABAR detector at the PEP-II B factory. We measure a partial branching fraction {Delta}{beta} in a restricted region of phase space that reduces the effect of theoretical uncertainties, requiring the lepton energy to be in the range 1.875 and 2.850 GeV, the photon energy to be in the range 0.45 and 2.35 GeV, and the cosine of the angle between the lepton and photon momenta to be less than -0.36, with all quantities computed in the {Upsilon}(4S) center-of-mass frame. We find {Delta}{Beta}(B{sup +} {yields} {gamma}{ell}{sup +}{nu}{sub {ell}}) = (-0.3{sub 1.5}{sup +1.3}(statistical){sub -0.6}{sup +0.6}(systematic) {+-} 0.1(theoretical)) x 10{sup -6}, under the assumption of lepton universality. Interpreted as a 90% confidence-level Bayesian upper limit, the result corresponds to 1.7 x 10{sup -6} for a prior at in amplitude, and 2.3 x 10{sup -6} for a prior at in branching fraction.
Date: October 6, 2008
Creator: Chen, Edward Tann & /Caltech
System: The UNT Digital Library
Authenticated group Diffie-Hellman key exchange: theory and practice (open access)

Authenticated group Diffie-Hellman key exchange: theory and practice

Authenticated two-party Diffie-Hellman key exchange allows two principals A and B, communicating over a public network, and each holding a pair of matching public/private keys to agree on a session key. Protocols designed to deal with this problem ensure A (B resp.)that no other principals aside from B (A resp.) can learn any information about this value. These protocols additionally often ensure A and B that their respective partner has actually computed the shared secret value. A natural extension to the above cryptographic protocol problem is to consider a pool of principals agreeing on a session key. Over the years several papers have extended the two-party Diffie-Hellman key exchange to the multi-party setting but no formal treatments were carried out till recently. In light of recent developments in the formalization of the authenticated two-party Diffie-Hellman key exchange we have in this thesis laid out the authenticated group Diffie-Hellman key exchange on firmer foundations.
Date: October 3, 2002
Creator: Chevassut, Olivier
System: The UNT Digital Library
Performance-Driven Interface Contract Enforcement for Scientific Components (open access)

Performance-Driven Interface Contract Enforcement for Scientific Components

Performance-driven interface contract enforcement research aims to improve the quality of programs built from plug-and-play scientific components. Interface contracts make the obligations on the caller and all implementations of the specified methods explicit. Runtime contract enforcement is a well-known technique for enhancing testing and debugging. However, checking all of the associated constraints during deployment is generally considered too costly from a performance stand point. Previous solutions enforced subsets of constraints without explicit consideration of their performance implications. Hence, this research measures the impacts of different interface contract sampling strategies and compares results with new techniques driven by execution time estimates. Results from three studies indicate automatically adjusting the level of checking based on performance constraints improves the likelihood of detecting contract violations under certain circumstances. Specifically, performance-driven enforcement is better suited to programs exercising constraints whose costs are at most moderately expensive relative to normal program execution.
Date: October 1, 2007
Creator: Dahlgren, T L
System: The UNT Digital Library
Constructing Predictive Estimates for Worker Exposure to Radioactivity During Decommissioning: Analysis of Completed Decommissioning Projects (open access)

Constructing Predictive Estimates for Worker Exposure to Radioactivity During Decommissioning: Analysis of Completed Decommissioning Projects

An analysis of completed decommissioning projects is used to construct predictive estimates for worker exposure to radioactivity during decommissioning activities. The preferred organizational method for the completed decommissioning project data is to divide the data by type of facility, whether decommissioning was performed on part of the facility or the complete facility, and the level of radiation within the facility prior to decommissioning (low, medium, or high). Additional data analysis shows that there is not a downward trend in worker exposure data over time. Also, the use of a standard estimate for worker exposure to radioactivity may be a best estimate for low complete storage, high partial storage, and medium reactor facilities; a conservative estimate for some low level of facility radiation facilities (reactor complete, research complete, pits/ponds, other), medium partial process facilities, and high complete research facilities; and an underestimate for the remaining facilities. Limited data are available to compare different decommissioning alternatives, so the available data are reported and no conclusions can been drawn. It is recommended that all DOE sites and the NRC use a similar method to document worker hours, worker exposure to radiation (person-rem), and standard industrial accidents, injuries, and deaths for all completed decommissioning …
Date: October 1, 2002
Creator: Dettmers, Dana Lee & Eide, Steven Arvid
System: The UNT Digital Library
Experimental Study of the Spin Structure of the Neutron (3He) at low Q2: a connection between the Bjorken and Gerasimov-Drell-Hearn Sum Rules (open access)

Experimental Study of the Spin Structure of the Neutron (3He) at low Q2: a connection between the Bjorken and Gerasimov-Drell-Hearn Sum Rules

The authors have presented the motivations in gathering doubly polarized data in the quasi-elastic, resonance and DIS domains. These data were used to calculate the extended GDH integral. The comparison of this quantity with the spin dependent forward Compton amplitude {bar S}{sub 1} is of particular importance for the unification of the two strong interaction descriptions (nucleonic/hadronic vs. partonic) because {bar S}{sub 1} is the first quantity theoretically calculable in the full Q{sup 2} domain of the strong interaction. Such a data taking was made possible because of three major technical achievements: (1) the beam of high duty cycle (100%), high current (up to 70 {micro}A) and high polarization (70%); (2) the {sup 3}He target of high density (above 10 atm) with a polarization of 35% and a length of 40 cm; and (3) the large acceptance (6 msr) and high resolution ({Delta}P/P {approx_equal} 10{sup {minus}4}) spectrometers. These features, available at Jefferson Lab, enabled them to achieve the highest luminosity in the world (about 10{sup 36} s{sup {minus}1} cm{sup {minus}2} with a current of 15 {micro}A) as far as polarized {sup 3}He targets are concerned. Consequently they were able to gather, in a rather short period of time (3 months), …
Date: October 9, 2000
Creator: Deur, Alexander
System: The UNT Digital Library
Polarization Transfer in the Reaction 4He(e,e'p)3H in the Quasielastic Scattering Region (open access)

Polarization Transfer in the Reaction 4He(e,e'p)3H in the Quasielastic Scattering Region

None
Date: October 1, 2003
Creator: Dieterich, Sonja
System: The UNT Digital Library
Search for super symmetry at the Tevatron using the trilepton signature (open access)

Search for super symmetry at the Tevatron using the trilepton signature

This dissertation describes a search for the associated production of the supersymmetric particles, the chargino and the neutralino, through their R-parity conserving decays to three leptons and missing energy. This search is carried out using the data collected at the CDF experiment at the Tevatron {radical}s = 1.96 TeV p{bar p} collider at Fermilab. The results are obtained by combining five independent channels with varying signal to background ratio. Overall, a total of 6.4 {+-} 1.1 background events from standard model processes and 11.4 {+-} 1.1 signal events for a particular choice of mSUGRA model parameters are expected. The observation of 7 events in data is consistent with the standard model background expectation, and the mSUGRA model is constrained. Limits are set on the cross section of Chargino-Neutralino pair production, and a limit on the mass of the chargino is extracted. A method of obtaining model-independent results is also discussed.
Date: October 1, 2008
Creator: Dube, Sourabh Shishir
System: The UNT Digital Library
Search for Charged Higgs in ttbar Decay Products from Proton-Antiproton Collisions at s**(1/2) = 1.96 TeV (open access)

Search for Charged Higgs in ttbar Decay Products from Proton-Antiproton Collisions at s**(1/2) = 1.96 TeV

This dissertation reports the results of a search for charged Higgs bosons in the decays of t{bar t} pairs produced in p{bar p} collisions at a center-of-mass energy of 1.96 TeV. The search is performed on a data sample recorded by the upgraded Collider Detector at Fermilab and corresponding to an integrated luminosity of 193 pb{sup -1}. The search is based on the relative rates of events in the different t{bar t} decay channels. Results are obtained in the context of different models. In the context of the minimal supersymmetric extension of the Standard Model (MSSM), for which they fully account for radiative and Yukawa coupling corrections, regions in the (m{sub H{sup {+-}}}, tan ({beta})) plane are excluded. In the Tauonic Higgs Model in which the charged Higgs is assumed to decay exclusively to {bar {tau}}, the BR(t {yields} H{sup +}b) is constrained to be less than 0.4 at 95% C.L. If no assumption is made on the charged Higgs decay, the BR(t {yields} H{sup +}b) is constrained to be less than 0.90 at 95% C.L. No evidence for charged Higgs production is found.
Date: October 1, 2005
Creator: Eusebi, Ricardo
System: The UNT Digital Library
Estimation of fracture porosity in an unsaturated fractured welded tuff using gas tracer testing (open access)

Estimation of fracture porosity in an unsaturated fractured welded tuff using gas tracer testing

None
Date: October 1, 2001
Creator: Freifeld, Barry
System: The UNT Digital Library
Experimental Studies of the Stimulated Brillouin Scattering Instability in the Saturated Regime (open access)

Experimental Studies of the Stimulated Brillouin Scattering Instability in the Saturated Regime

An experimental study of the stimulated Brillouin scattering (SBS) instability has investigated the effects of velocity gradients and kinetic effects on the saturation of ion-acoustic waves in a plasma. For intensities less than I < 1.5 x 10{sup 15} W cm{sup -2}, the SBS instability is moderated primarily by velocity gradients, and for intensities above this threshold, nonlinear trapping is invoked to saturate the instability. We report direct evidence of detuning of SBS by a velocity gradient which was achieved by directly measuring the frequency shift of the SBS driven acoustic wave relative to the local resonant acoustic frequency. Furthermore, a novel use of Thomson scattering has allowed us to gather direct evidence of kinetic effects associated with the SBS process. Specifically, a measured two-fold increase of the ion temperature has been linked with laser beam excitation of ion-acoustic waves to large amplitudes by the SBS instability. Ion-acoustic waves were excited to large amplitude with a 2{omega} 1.2-ns long interaction beam with intensities up to 5 x 10{sup 15} W cm{sup -2}. The local frequency, amplitude, and spatial range of these waves were measured with a 3{omega} 200ps Thomson-scattering probe beam. These detailed and accurate measurements in well-characterized plasma conditions …
Date: October 29, 2002
Creator: Froula, D
System: The UNT Digital Library
Scalable Performance Measurement and Analysis (open access)

Scalable Performance Measurement and Analysis

Concurrency levels in large-scale, distributed-memory supercomputers are rising exponentially. Modern machines may contain 100,000 or more microprocessor cores, and the largest of these, IBM's Blue Gene/L, contains over 200,000 cores. Future systems are expected to support millions of concurrent tasks. In this dissertation, we focus on efficient techniques for measuring and analyzing the performance of applications running on very large parallel machines. Tuning the performance of large-scale applications can be a subtle and time-consuming task because application developers must measure and interpret data from many independent processes. While the volume of the raw data scales linearly with the number of tasks in the running system, the number of tasks is growing exponentially, and data for even small systems quickly becomes unmanageable. Transporting performance data from so many processes over a network can perturb application performance and make measurements inaccurate, and storing such data would require a prohibitive amount of space. Moreover, even if it were stored, analyzing the data would be extremely time-consuming. In this dissertation, we present novel methods for reducing performance data volume. The first draws on multi-scale wavelet techniques from signal processing to compress systemwide, time-varying load-balance data. The second uses statistical sampling to select a small …
Date: October 27, 2009
Creator: Gamblin, T
System: The UNT Digital Library
B Quark Tagging and Cross-Section Measurement in Quark Pair Production at d0 (open access)

B Quark Tagging and Cross-Section Measurement in Quark Pair Production at d0

None
Date: October 1, 2004
Creator: Greder, Sebastien & /Louis Pasteur U., Strasbourg I /Strasbourg, IReS
System: The UNT Digital Library
Galaxy Evolution Insights from Spectral Modeling of Large Data Sets from the Sloan Digital Sky Survey (open access)

Galaxy Evolution Insights from Spectral Modeling of Large Data Sets from the Sloan Digital Sky Survey

This thesis centers on the use of spectral modeling techniques on data from the Sloan Digital Sky Survey (SDSS) to gain new insights into current questions in galaxy evolution. The SDSS provides a large, uniform, high quality data set which can be exploited in a number of ways. One avenue pursued here is to use the large sample size to measure precisely the mean properties of galaxies of increasingly narrow parameter ranges. The other route taken is to look for rare objects which open up for exploration new areas in galaxy parameter space. The crux of this thesis is revisiting the classical Kennicutt method for inferring the stellar initial mass function (IMF) from the integrated light properties of galaxies. A large data set ({approx} 10{sup 5} galaxies) from the SDSS DR4 is combined with more in-depth modeling and quantitative statistical analysis to search for systematic IMF variations as a function of galaxy luminosity. Galaxy H{alpha} equivalent widths are compared to a broadband color index to constrain the IMF. It is found that for the sample as a whole the best fitting IMF power law slope above 0.5 M{sub {circle_dot}} is {Lambda} = 1.5 {+-} 0.1 with the error dominated by …
Date: October 1, 2007
Creator: Hoversten, Erik A.
System: The UNT Digital Library
A search for z boson pair production at the Fermilab Tevatron Collider (open access)

A search for z boson pair production at the Fermilab Tevatron Collider

This dissertation describes a search for Z/{gamma}* boson pair production decaying into {mu}{mu}{mu}{mu}, {mu}{mu}ee, and eeee final states with approximately 1 fb{sup -1} of data at the Fermilab Tevatron Collider at {radical}s = 1.96 TeV. The small cross section times branching ratio for each channel mandated a thorough study of the acceptance and efficiencies. After optimization, 1.7 {+-} 0.1 events are expected for Standard Model production with a background of 0.13 {+-} 0.03 events. One event was found in the {mu}{mu}ee channel. A cross section limit of 4.4 pb is determined at a 95% confidence level for Standard Model production. Additionally, one parameter and two parameter 95% C.L. limits are found for the anomalous neutral trilinear gauge couplings ZZZ* and ZZ{gamma}*. The one parameter 95% C.L. coupling limits with a form factor scale of 1.2 TeV are: -0.28 < f{sub 40}{sup Z} < 0.28, -0.31 < f{sub 50}{sup Z} < 0.29, -0.26 < f{sub 40}{gamma} < 0.26, and -0.30 < f{sub 50}{sup {gamma}} < 0.28.
Date: October 1, 2007
Creator: Jarvis, Chad Ryan
System: The UNT Digital Library
Amorphous and nanocrystalline phase formation in highly-driven Al-based binary alloys (open access)

Amorphous and nanocrystalline phase formation in highly-driven Al-based binary alloys

Remarkable advances have been made since rapid solidification was first introduced to the field of materials science and technology. New types of materials such as amorphous alloys and nanostructure materials have been developed as a result of rapid solidification techniques. While these advances are, in many respects, ground breaking, much remains to be discerned concerning the fundamental relationships that exist between a liquid and a rapidly solidified solid. The scope of the current dissertation involves an extensive set of experimental, analytical, and computational studies designed to increase the overall understanding of morphological selection, phase competition, and structural hierarchy that occurs under far-from equilibrium conditions. High pressure gas atomization and Cu-block melt-spinning are the two different rapid solidification techniques applied in this study. The research is mainly focused on Al-Si and Al-Sm alloy systems. Silicon and samarium produce different, yet favorable, systems for exploration when alloyed with aluminum under far-from equilibrium conditions. One of the main differences comes from the positions of their respective T{sub 0} curves, which makes Al-Si a good candidate for solubility extension while the plunging T{sub 0} line in Al-Sm promotes glass formation. The rapidly solidified gas-atomized Al-Si powders within a composition range of 15 to 50 …
Date: October 15, 2008
Creator: Kalay, Yunus Eren
System: The UNT Digital Library
Measurement of the W + gamma Production in Proton - Anti-proton Collisions at s**(1/2) = 1.96-TeV (open access)

Measurement of the W + gamma Production in Proton - Anti-proton Collisions at s**(1/2) = 1.96-TeV

The authors present a measurement of the {bar p}p {yields} W{gamma} + X {yields} e{nu}{gamma} + X production cross section using data form the Collider Detector at Fermilab. The p{bar p} collisions were provided by the Tevatron Collider at a center of mass energy of 1.96 TeV. Electroweak theory includes the trilinear vector boson coupling, WW{gamma}, which contributes to the e{nu}{gamma} final state. The electron decay channel of the W provides a clean sample to study the production of diboson pairs. The measurement of the production cross section tests the structure of the non-Abelian character of Electroweak theory.
Date: October 1, 2004
Creator: Kirby, Michael H. & U., /Duke
System: The UNT Digital Library
Large Pt processes in ppbar collisions at 2 TeV: measurement of ttbar production cross section in ppbar collisions at s**(1/2) = 1.96 TeV in the dielectron final states at the D0 experiment (open access)

Large Pt processes in ppbar collisions at 2 TeV: measurement of ttbar production cross section in ppbar collisions at s**(1/2) = 1.96 TeV in the dielectron final states at the D0 experiment

The measurement of the top-antitop pair production cross section in p{bar p} collisions at {radical}s = 1.96 TeV in the dielectron decay channel using 384 pb{sup -1} of D0 data yields a t{bar t} production cross-section of {sigma}{sub t{bar t}} = 7.9{sub -3.8}{sup +5.2}(stat){sub -1.0}{sup +1.3}(syst) {+-} 0.5 (lumi) pb. This measurement [98] is based on 5 observed events with a prediction of 1.04 background events. The cross-section corresponds to the top mass of 175 GeV, and is in good agreement with the Standard Model expectation of 6.77 {+-} 0.42 pb based on next-to-next-leading-order (NNLO) perturbative QCD calculations [78]. This analysis shows significant improvement from our previous cross-section measurement in this channel [93] with 230 pb{sup -1} dataset in terms of significantly better signal to background ratio and uncertainties on the measured cross-section. Combination of all the dilepton final states [98] yields a yields a t{bar t} cross-section of {sigma}{sub t{bar t}} = 8.6{sub -2.0}{sup +2.3}(stat){sub -1.0}{sup +1.2}(syst) {+-} 0.6(lumi) pb, which again is in good agreement with theoretical predictions and with measurements in other final states. Hence, these results show no discernible deviation from the Standard Model. Fig. 6.1 shows the summary of cross-section measurements in different final states …
Date: October 1, 2005
Creator: Kumar, Ashish & U., /Delhi
System: The UNT Digital Library
Applications of a single-molecule detection in early disease diagnosis and enzymatic reaction study (open access)

Applications of a single-molecule detection in early disease diagnosis and enzymatic reaction study

Various single-molecule techniques were utilized for ultra-sensitive early diagnosis of viral DNA and antigen and basic mechanism study of enzymatic reactions. DNA of human papilloma virus (HPV) served as the screening target in a flow system. Alexa Fluor 532 (AF532) labeled single-stranded DNA probes were hybridized to the target HPV-16 DNA in solution. The individual hybridized molecules were imaged with an intensified charge-coupled device (ICCD) in two ways. In the single-color mode, target molecules were detected via fluorescence from hybridized probes only. This system could detect HPV-16 DNA in the presence of human genomic DNA down to 0.7 copy/cell and had a linear dynamic range of over 6 orders of magnitude. In the dual-color mode, fluorescence resonance energy transfer (FRET) was employed to achieve zero false-positive count. We also showed that DNA extracts from Pap test specimens did not interfere with the system. A surface-based method was used to improve the throughput of the flow system. HPV-16 DNA was hybridized to probes on a glass surface and detected with a total internal reflection fluorescence (TIRF) microscope. In the single-probe mode, the whole genome and target DNA were fluorescently labeled before hybridization, and the detection limit is similar to the flow …
Date: October 15, 2008
Creator: Li, Jiangwei
System: The UNT Digital Library
THE APPLICATION OF SINGLE PARTICLE AEROSOL MASS SPECTROMETRY FOR THE DETECTION AND IDENTIFICATION OF HIGH EXPLOSIVES AND CHEMICAL WARFARE AGENTS (open access)

THE APPLICATION OF SINGLE PARTICLE AEROSOL MASS SPECTROMETRY FOR THE DETECTION AND IDENTIFICATION OF HIGH EXPLOSIVES AND CHEMICAL WARFARE AGENTS

Single Particle Aerosol Mass Spectrometry (SPAMS) was evaluated as a real-time detection technique for single particles of high explosives. Dual-polarity time-of-flight mass spectra were obtained for samples of 2,4,6-trinitrotoluene (TNT), 1,3,5-trinitro-1,3,5-triazinane (RDX), and pentaerythritol tetranitrate (PETN); peaks indicative of each compound were identified. Composite explosives, Comp B, Semtex 1A, and Semtex 1H were also analyzed, and peaks due to the explosive components of each sample were present in each spectrum. Mass spectral variability with laser fluence is discussed. The ability of the SPAMS system to identify explosive components in a single complex explosive particle ({approx}1 pg) without the need for consumables is demonstrated. SPAMS was also applied to the detection of Chemical Warfare Agent (CWA) simulants in the liquid and vapor phases. Liquid simulants for sarin, cyclosarin, tabun, and VX were analyzed; peaks indicative of each simulant were identified. Vapor phase CWA simulants were adsorbed onto alumina, silica, Zeolite, activated carbon, and metal powders which were directly analyzed using SPAMS. The use of metal powders as adsorbent materials was especially useful in the analysis of triethyl phosphate (TEP), a VX stimulant, which was undetectable using SPAMS in the liquid phase. The capability of SPAMS to detect high explosives and CWA …
Date: October 23, 2006
Creator: Martin, A
System: The UNT Digital Library