In this experiment, fluorescent N- (1-pyrenyl) iodoacetamide modified the two reactive thiols, SH1 (Cys 707) and SH2 (Cys 697) on myosin to detect SH1-SH2 a -helix melting. The excimer forming property of pyrene is well suited to monitor the dynamics of the SH1 and SH2 helix melting, since the excimer should only form during the melted state. Decreased anisotropy of the excimer relative to the monomeric pyrene fluorescence is consistent with the disordering of the melted SH1-SH2 region in the atomic model. Furthermore, nucleotide analogs induced changes in the anisotropy of the excimer, suggesting that the nucleotide site modulates the flexibility of SH1-SH2 region.

The purpose of this study is to examine the relationship between the independent variables of age, gender, residence, tribal affiliation, and perceived government control over tribal rights and the dependent variables of hopelessness, self-efficacy, and self-esteem. These attitudes are then explored as to their relationship to possible feelings of powerlessness among American Indians. The survey instruments used are the Beck Hopelessness Scale consisting of 20 items (Beck, Weissman, Lester, and Trexler, 1974), (Reproduced by permission of publisher, Psychological Corporation), the Self-Efficacy Scale consisting of 30 items (Sherer, Maddox, Merchandante, Prentice-Dunn, Jacobs, and Rodgers, 1982) (Reproduced by permission of Dr. Ronald W. Rogers), the Rosenberg Self-Esteem Scale consisting of 10 items (Rosenberg, 1972) (Reproduced by permission of Dr. Florence Rosenberg) and a demographic questionnaire consisting of 6 items. These instruments were administered to 60 American Indians that make up the sample population of 25 respondents from tribal lands (reservation setting) and 35 respondents from an urban setting. Statistical analysis consists of crosstabulations using Chi-Square and t-tests (used to verify Chi-Square) to determine the significance of the relationship of the independent variables to the dependent variables previously mentioned. Fifteen hypotheses (page 10) were tested to explore the relationships between the above independent …

The effect of substituents on the stabilities of cumulenes-ketenes, allenes, diazomethanes and isocyanates and related systems-alkynes, nitriles and nitrile oxides is studied using the density functional theory (B3LYP, SVWN and BP86) and ab initio (HF, MP2) calculations at the 6-31G* basis set level. Using isodesmic reactions, correlation between stabilization energies of cumulenes and substituent group electronegativities (c BE) is established and the results from DFT and MP2 methods are compared with the earlier HF calculations. Calculations revealed that the density functional methods can be used to study the effect of substituents on the stabilities of cumulenes. It is observed that the cumulenes are stabilized by electropositive substituent groups from s -electron donation and p -electron withdrawal and are destabilized by electronegative substituent groups from n-p donation. The calculated geometries of the cumulenes are compared with the available experimental data.High level ab initio and density functional theory calculations have been used to study the energetics of low-barrier hydrogen bond (LBHB) systems. Using substituted formic acid-formate anion complexes as model LBHB systems, hydrogen bond strength is correlated to the pKa mismatch between the hydrogen bond donor and the hydrogen bond acceptor. LBHB model systems are characterized by the 1H-NMR chemical shift calculations. …

Since there are few studies which address the relationships between racial/ethnic identity and acculturation in the African-American community, the purpose of this study was threefold: 1) explore the relationship between racial identity and African-American acculturation; 2) examine racial and ethnic identity associations; and 3) observe the connections between these cultural constructs and psychological well-being. One hundred ninety-four African-American undergraduates from a predominantly White institution and two historically Black colleges completed measures of these constructs, self-esteem, and depression. The findings indicate a relationship between racial identity and acculturation for three of the four Cross (1971) stages (encounter, immersion-emersion, and internalization). Relinquishing the White frame of reference and achieving inner security with their Blackness coincides with immersion in the eight facets comprising African-American culture. Individuals who do not identify with their race (pre-encounter) less often affiliated with their ethnic group. Conversely, achieving racial identity (internalization) was associated with ethnic identity attachment. Finally, the study's findings suggest that identity development may affect how individuals perceive themselves and feel emotionally, which may depend on identity achievement. Pre-encounter stage scores were associated with reports of higher depression and lower self-esteem; whereas, higher internalization individuals reported higher self-esteem. As for ethnic identity, those who have explored …

Few studies have investigated methods for establishing neutral stimuli as conditioned reinforcers in human subjects. Conditioned reinforcers, however, can alleviate some of the problems encountered in applied behavior analytic (ABA) therapy for children with autism, such as satiation and suitability of reinforcers for specific environments. A series of reversals evaluated the effects of a conditioning procedure involving pairing a neutral stimulus, the remote control stimulus (RCT), with an identified reinforcer. Phase 1 demonstrated that the RCT was neutral. In Phase 2, alternating pairing and testing conditions were run. During testing the effects of pairing were evaluated by the effectiveness of the RCT in maintaining a response in the absence of a previously available reinforcer (extinction test) and in increasing a new response over a baseline level (learning test). Results from the extinction test suggest that under some pairing conditions the RCT can acquire properties of a reinforcer.

The dissertation is a collection of creative and non-fiction work, including a novel with critical introduction, four short stories, and three essays. The novel is a modern day Grail quest that takes place primarily in the Southwestern United States. The short stories are mostly set in the southwest as well, and take for their topic what Paul Fussel refers to as "hope abridged." The essays are non-fiction.

Paenibacillus velaei, sp. nov. is a soil bacterium capable of producing an unusually large amount of exopolysaccharide (EPS). The EPS contains glucose, mannose, galactose and fucose in a molar ratio of 4:2:1:1. The molecular weight of the EPS is higher than 2x106. The viscosity of 1% EPS is 1300 cP when measured at a shear rate of 1 sec-1. Physiological parameters for optimal production of the EPS were studied and it was found that 1.4 g dry weight per 1 l of medium was produced when the bacteria were grown at 30EC and the pH adjusted at 7± 0.2 in a medium containing glucose as the carbon source. Growing the bacteria on different carbon sources did not alter the quantity or the composition of the EPS produced. No toxicity effects were observed in mice or rats when EPS was administered in amounts ranging from 20 to 200 mg per kg body weight. The data obtained from physical, chemical and biological properties suggest that the EPS may be employed in several industrial and environmental applications. It is an excellent emulsifier, it holds 100 times its own weight in water, it is not toxic, and it can be used to remove mercury, …

This research investigated physician and emergency room use among representative samples of children in the Dallas metropolitan area (N = 1606) and among patients who used Children's Medical Center of Dallas' First Care services (N = 612). Through telephone interviewing, caregivers to children under fifteen years of age were asked about an array of health service use behaviors, social-psychological issues related to acquiring health care for their children, and demographic characteristics as outlined by the Andersen & Newman model of health care service use. Children's use of physician services is best predicted by whether or not they have medical insurance, their level of income, and whether or not they have medical homes. Although having commercial managed care and fee-for-service Medicaid insurance consistently predicted increased physician use, neither independently reduced reliance on emergency rooms for non-emergent care. Managed care insurance and Medicaid did, however, significantly improve the odds that children would have medical homes, which significantly decreased emergency room use for non-emergent care. Further, increasing physician use and reducing reliance on hospital emergency rooms for non-emergent care will require ensuring that children have medical homeseither private physicians or community health centersat which they can readily and consistently receive sick and well …

Throughout history floods have been part of the human experience. They are recurring phenomena that form a necessary and enduring feature of all river basin and lowland coastal systems. In an average year, they benefit millions of people who depend on them. In the more developed countries, major floods can be the largest cause of economic losses from natural disasters, and are also a major cause of disaster-related deaths in the less developed countries. Flood disaster mitigation research was conducted to determine how remotely sensed data can effectively be used to produce accurate flood plain maps (FPMs), and to identify/quantify the sources of error associated with such data. Differences were analyzed between flood maps produced by an automated remote sensing analysis tailored to the available satellite remote sensing datasets (rFPM), the 100-year flooded areas "predicted" by the Flood Insurance Rate Maps, and FPMs based on DEM and hydrological data (aFPM). Landuse/landcover was also examined to determine its influence on rFPM errors. These errors were identified and the results were integrated in a GIS to minimize landuse / landcover effects. Two substantial flood events were analyzed. These events were selected because of their similar characteristics (i.e., the existence of FIRM or …

Although research has shown that the complex constructs of intelligence and personality are necessarily intertwined, studies exploring this issue in elderly individuals are rare. The importance attached to this interface in older adults becomes particularly clear in light of the debate over the cause and extent of age-related decrements in cognitive performance as well as whether such losses can be ameliorated or not, especially given societal shifts toward increased life expectancies. The present study explored the basis for shifts in personality-ability relationships in adulthood by comparing two samples of older adults, one of which was assessed in 1975 (N = 102, M age = 68.4), and the second of which was assessed in 1995 (N = 100, M age = 72.0), and a sample of younger adults (N = 100, M age = 21.8), also assessed in 1995. Each participant was administered the Holtzman Inkblot Technique and the Gf-Gc Sampler, a measure of crystallized (Gc) and fluid (Gf) abilities. LISREL analyses of both age-related and historical shifts in personality-ability relationships suggested that not only were such shifts associated with cohort differences as reflected in factor loading (lambda) differences between the older samples and the younger sample, as well as between …

The life history and case-making behaviors of Phylloicus ornatus from two springfed first order streams in the Edwards Plateau Bioregion of Texas were studied from January 1998 to November 1999. Field larval, pupal and adult samples and laboratory rearings indicated a multivoltine cycle. First instars differ from late instars in number of labral setae and in having a unique spur-like claw on each lateral hump. Larval development was asynchronous with second through fifth instars and pupae present most months. First instars were present April through July, October and November. Case making of first instar and case reconstruction of later instars extracted from their cases was documented by videophotography.

This research explores the relationship of self-monitoring with team leader behavior and work environment preference. Those who are high on self-monitoring demonstrate flexibility in their actions with others and are socially perceptive. They perform well in a variety of leadership positions and are viewed as leaders by group members. High self-monitoring types choose "socially" based careers, including teacher and psychologist, in which they adapt their interaction styles to effectively meet the demands of clients. The demands placed on a team leader appear to require similar characteristics to those that high self-monitoring individuals possess. As a team matures through different stages of development, the role of the leader ranges from director to facilitator to consultant. In order to effectively meet team needs, a leader must be socially sensitive to interpersonal cues and have the ability to assume various roles. In addition, given the fact that the position of team leader is a highly social type of career that requires behaviors similar to careers chosen by high self-monitoring individuals, it is likely that high self-monitors would prefer working in a team work environment over a traditional one. A survey methodology was used to assess the characteristics of 100 team members. No relationship …

I analyze the behavior of Jews imprisoned in the Treblinka, Sobibór, and Birkenau extermination camps in order to illustrate a systematic process of deception and psychological conditioning, which the Nazis employed during World War II to preclude Jewish resistance to the Final Solution. In Chapter I, I present resistance historiography as it has developed since the end of the war. In Chapter II, I delineate my own argument on Jewish behavior during the Final Solution, limiting my definition of resistance and the applicability of my thesis to behavior in the extermination camp, or closed, environment. In Chapters III, IV, and V, I present a detailed narrative of the Treblinka, Sobibór, and Birkenau revolts using secondary sources and selected survivor testimony. Finally, in Chapter VI, I isolate select parts of the previous narratives and apply my argument to demonstrate its validity as an explanation for Jewish behavior.

In July of 1999, I wrote to 24 Texas junior and community colleges (and one four-year institution) describing my thesis agenda of a general biology lecture and laboratory syllabus for introductory biology students. I requested the titles and authors of the general biology textbooks and laboratory manuals they were currently using, the publishers of these texts, and the edition of said texts. I then contacted publishers of the various textbooks who, in turn, directed me to the Dallas-area representatives for further inquiries. I assimilated the various authors' general biology topics into a two-semester syllabus of lecture and one semester of laboratory. The document is not a text manuscript, but an all-inclusive listing of a general biology syllabus broken down by subject.

Since abolition, women have used the media to bring attention to causes and injustices in society. Issues faced by these women are some of the same issues faced by women in public relations today and possibly the future. This paper is the history of the women of pre-professional public relations in relation to their use of the media to bring about change and communicate with an audience. It also discusses the evolution of the public relations profession as it pertains to the parallel issues that the women of the first wave faced in relation to the second wave, or professional era. The paper will then synthesize these two eras in public relations and discuss the future of women in the profession as seen by researchers and women practicing at this time.

The purpose of this study was to analyze students' specific ITP-related social skills goals, student self-reported social skills, and the relationship between teacher and employer ratings of vocational students social skills and problem behaviors. This study examined (48) vocational students, (24) general education vocational students and (24) special education vocational students in grades nine through twelve. The students' vocational teachers and employers also participated in the study. This represented (144) individual assessment of social skills and problem behaviors utilizing the Social Skills Rating System -Student version (SSRS-S) and the Social Skills Rating System Teachers -version (SSRS-T). The findings indicated no specific social skill goals were deliminated in the students' ITP's. However, the findings did indicate the general education vocational students rated themselves higher, on average, on the empathy subscale than did the special education students. The analysis of data comparing standardized social skill scores, social skill subscale scores, standardized problem behavior scores, and standardized problem behavior subscale scores between teachers and employers for general and special education vocational students indicated employers rated special education students higher on the cooperation subscale only. No other differences were found.

This study examined consumer attitude towards adding branded quick-service items on domestic airline in-flight menus with the aim of assessing the variables of perceived customer value and customer satisfaction. A random sample of one hundred sixteen frequent flyers residing in the United States participated in the study. An examination of consumer attitude towards branded quick-service menu items if introduced on domestic in-flight menus was performed. Multiple regression analysis was used to determine which of the four relationships was the most significant. The results showed that frequent flyer satisfaction with branded quick-service food items had the most significant relationship with perceived satisfaction of adding branded quick-service foods to coach class in-flight meal service.

An examination of the compositional style illustrative of the anti-collectivist ideology as found in Edison Denisov's Quatre Pièces pour flûte et piano. Includes a short history of Denisov's formal training, history of the Soviet musical environment, an overview of his creative output, and discussion of the anti-collectivist characteristics in his works. Defines the anti-collectivist doctrine as individual reaction to the totalitarian collective of the Soviet communist state of the twentieth century. Identification of eclectic compositional techniques, and how they represent individual expression under a totalitarian regime. Listing of Denisov's works with the flute in a primary role, interviews with Aurèle Nicolet and Ekaterina Denisov, correspondence from Denisov to Nicolet, and the manuscript score to Quatre Pièces pour flûte et piano follow as appendices.

This dissertation has explored the origins of the field of Behavioral Disorders via a qualitative approach. In order to collect data, interviews were conducted with respondents who were selected via purposeful sampling. All respondents have had a significant impact on the field of special education as evidenced by scholarship and leadership throughout their careers. Data analysis of the interview transcriptions was accomplished through the utilization of computer software. The data indicated six areas/topics that were seen among respondents as being significant to the development of the field of Behavioral Disorders.

Mutations in BRCA1 and BRCA2, the first two breast cancer susceptibility genes identified, carry as much as an 85% lifetime risk of developing breast, ovarian or other cancers. Genetic testing for mutations in these two genes has recently become commercially available. There have been varying amounts of psychological distress noted among women with a family history of breast cancer. Distress has been observed to impact psychological functioning, activities of daily living, and the practice of breast cancer surveillance behaviors. Within the genetic screening process, psychological distress has been shown to impact the decision to undergo genetic screening, the comprehension and retention of risk assessment information, as well as affecting the subject following the receipt of the genetic test results. Little work has been done to examine predictors of distress within at risk subjects. This study examines psychological distress among 52 community women presenting for BRCA1 and BRCA2 genetic mutation testing. Predictors of distress included family cancer history, education, age, Ashkenazi ethnicity, and Internality and Powerful Others Health Locus of Control. Vulnerable sub-groups of patients include younger women, women with higher levels of education and women of Ashkenazi ethnicity.

The changing role of women was arguably the most fundamental area of concern and crisis in the Victorian era. Recent scholarship has done much to illuminate the evolving role of women, particularly in regard to the development of the New Woman. I propose that there is an intermediary character type that exists between Coventry Patmore's "angel of the house" and the New Woman of the fin de siecle. I call this character the Wandering Woman. This new archetypal character adheres to the following list of characteristics: she is a literal or figurative orphan, is genteelly poor or of the working class, is pursued by a rogue who offers financial security in return for sexual favors; this sexual liaison, unsanctified by marriage, causes her to be stigmatized in the eyes of society; and her stigmatization results in expulsion from society and enforced wandering through a literal or figurative wilderness. There are three variations of this archetype: the child-woman as represented by the titular heroine of Charlotte Bronte's Jane Eyre and Little Nell of Charles Dickens' The Old Curiosity Shop; the sexual deviant as represented by Miss Wade of Dickens' Little Dorrit; and the fallen woman as represented by the titular heroine …

A model for health care utilization was applied to a sample of low income women. Demographic Predisposing, Psychosocial Predisposing, Illness Level, and Enabling indicators were examined separately for African American (n = 266), Anglo American (n = 200), and Mexican American (n = 210) women. Structural Equation Modeling revealed that for African American and Anglo American women, Illness Level, the only significant path to Utilization, had a mediating effect on Psychosocial Predisposing indicators. The model for Mexican Americans was the most complex with Enabling indicators affecting Illness Level and Utilization. Psychosocial Predisposing indicators were mediated by Illness Level and Enabling indicators which both directly affected Utilization. Implications of the results for future research are addressed.

My dissertation examines the roles of Emily and Anne Brontë as nineteenth-century women poets, composing in a literary form dominated by androcentric language and metaphor. The work of Mikhail Bakhtin, particularly concerning spoken and implied dialogue, and feminists who have pioneered an exploration of feminist dialogics provide crucial tools for examining the importance and uses of the dialogic form in the development of a powerful and creative feminine voice. As such, I propose to view Emily's Gondal poetry not as a series of loosely connected monologues, but as utterances in an inner dialogue between the dissenting and insistent female voice and the authoritative voice of the non-Gondal world. Emily's identification with her primary heroine, Augusta, enables her to challenge the controlling voice of the of the patriarchy that attempts to dictate and limit her creative and personal expression. The voice of Augusta in particular expresses the guilt, shame, and remorse that the woman-as-author must also experience when attempting to do battle with the patriarchy that attempts to restrict and reshape her utterances. While Anne was a part of the creation of Gondal, using it to mask her emotions through sustained dialogue with those who enabled and inspired such feelings, her …

In this research, the necessity of understanding and using bioinformatics is demonstrated using the enzyme aspartate transcarbamoylase (ATCase) as the model enzyme. The first portion of this research focuses on the use of bioinformatics. A partial sequence of the pyrB gene found in Enterococcus faecalis was submitted to GenBank and was analyzed against the contiguous sequence from its own genome project. A BLAST (Basic Local Alignment Search Tool; Atschul, et al., 1990) was performed in order to hypothesize the remaining portion of the gene from the contiguous sequence. This allowed a global comparison to other known aspartate transcarbamoylases (ATCases) and once deduced, a translation of the sequence gave the stop codon and thus the complete sequence of the open reading frame. When this was complete, upstream and downstream primers were designed in order to amplify the gene from genomic DNA. The amplified product was then sequenced and used later in phylogenetic analyses concerning the evolution of ATCase. The second portion of this research involves taking multiple ATCase nucleotide sequences and performing phenetic and phylogenetic analyses of the archaea and eubacter families. From these analyses, ancestral relationships which dictate both structure and function were extrapolated from the data and discussed.