Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for studying bi-molecular interactions at the atomic scale. Our NMR lab is involved in the identification of small molecules, or ligands that bind to target protein receptors, such as tetanus (TeNT) and botulinum (BoNT) neurotoxins, anthrax proteins and HLA-DR10 receptors on non-Hodgkin's lymphoma cancer cells. Once low affinity binders are identified, they can be linked together to produce multidentate synthetic high affinity ligands (SHALs) that have very high specificity for their target protein receptors. An important nanotechnology application for SHALs is their use in the development of robust chemical sensors or biochips for the detection of pathogen proteins in environmental samples or body fluids. Here, we describe a recently developed NMR competition assay based on transferred nuclear Overhauser effect spectroscopy (trNOESY) that enables the identification of sets of ligands that bind to the same site, or a different site, on the surface of TeNT fragment C (TetC) than a known ''marker'' ligand, doxorubicin. Using this assay, we can identify the optimal pairs of ligands to be linked together for creating detection reagents, as well as estimate the relative binding constants for ligands competing for the same site.

We review the phase diagram of nitrogen in a wide pressure and temperature range. Recent optical and x-ray diffraction studies at pressures up to 300 GPa and temperatures in excess of 1000 K have provided a wealth of information on the transformation of molecular nitrogen to a nonmolecular (polymeric) semiconducting and two new molecular phases. These newly found phases have very large stability (metastability) range. Moreover, two new molecular phases have considerably different orientational order from the previously known phases. In the iota phase (unlike most of other known molecular phases), N{sub 2} molecules are orientationally equivalent. The nitrogen molecules in the theta phase might be associated into larger aggregates, which is in line with theoretical predictions on polyatomic nitrogen.

This booklet was prepared and distributed by the Brain Injury Association of Texas to answer the questions of brain injured persons' family, friends, and caretakers.

Interview with community activist Gloria Villanueva-Anderson. The interview includes Villanueva-Anderson's personal experiences about being an activist in the Mexican-American community of Denton, Texas, education in Denton schools, discrimination at the train station in Denison, Texas, being accepted to the work-scholarship program of the FBI in 1952, opening her telephone answering exchange business, turning toward Republican politics, and her activities with George H.W. Bush's Texas Statewide Hispanic Campaign. Additionally, Villanueva-Anderson discusses her family background, the lack of discrimination against Hispanics in Denton, her family's assimilation in the Anglo culture, early Hispanic families in Denton, her appointment to the North Texas Hispanic Advisory Board by Senator John Tower, as well as her appointments to the Texas Small Business Task Force by Governor William Clements, the White House Conference on Small Business by President Jimmy Carter, and as Regional Advocate for the Small Business Administration by Ronald Reagan.

This document is a review report noting 39 commendable practices and made 68 recommendations for improvements of the Marble Falls Independent School District(MFISD).

This document provides information about vaccine and immunization requirements for children and students in the state of Texas.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.