A prototype oligonucleotide ''functional chip'' has been developed to screen novel DNA repair proteins for their ability to bind or alter different forms of DNA. This chip has been developed as a functional genomics screen for analysis of protein-DNA interactions for novel proteins identified from the Human Genome Project The process of novel gene identification that has ensued as a consequence of available sequence information is remarkable. The challenge how lies in determining the function of newly identified gene products in a time-and cost-effective high-throughput manner. The functional chip is generated by the robotic application of DNA spotted in a microarray format onto a glass slide. Individual proteins are then analyzed against the different form of DNA bound to the slide. Several prototype functional chips were designed to contain various DNA fragments tethered to a glass slide for analysis of protein-DNA binding or enzymatic activity of known proteins. The technology has been developed to screen novel, putative DNA repair proteins for their ability to bind various types of DNA alone and in concert with protein partners. An additional scheme has been devised to screen putative repair enzymes for their ability to process different types of DNA molecules. Current methods to …

This document updates the Version Description Document with the changes incorporated in the Revision 11.2 software installation on the Tank Monitor and Control System (TMACS).

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Laboratory facilities have made great strides in producing large sets of reliable data for X-ray astronomy, which include ionization and recombination cross sections needed for charge balance calculations as well as the atomic data needed for interpreting X-ray line formation. We discuss data from the new generation sources and pay special attention to the LLNL electron beam ion trap experiment, which is unique in it's ability to provide direct laboratory access to spectral data under precisely controlled conditions that simulate those found in many astrophysical plasmas. Examples of spectral data obtained in the 1-160 A wavelength range are given illustrating the type of laboratory X-ray data produced in support of such missions as Chandra, XMM, ASCA and EUVE.

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The D-Zero crane rail is analyzed for use as an anchor support for a one person Horizon{trademark} Horizontal Lifeline system that will span the pit area at D-Zero assembly hall. The lifeline will span 75 ft across the pit area, will be located out of the travel of the crane and above the concrete lentil wall. The crane rail is a suitable anchor for a one person Horizon TM Horizontal Lifeline system. The expected stress on the rail is 1,995 psi which has a factor of safety of 5.5 on the allowable stress. The anchor position is located 18 feet away from the concrete lentil wall and out of the travel of the overhead crane.

This report summarizes the results of Phase 2 of this contract. The authors completed four tasks under this phase of the subcontract. (1) They developed a computational fluid dynamics (CFD) model of a 3500 direct injected natural gas (DING) engine gas injection/combustion system and used it to identify DING ignition/combustion system improvements. The results were a 20% improvement in efficiency compared to Phase 1 testing. (2) The authors designed and procured the components for a 3126 DING engine (300 hp) and finished assembling it. During preliminary testing, the engine ran successfully at low loads for approximately 2 hours before injector tip and check failures terminated the test. The problems are solvable; however, this phase of the program was terminated. (3) They developed a Decision & Risk Analysis model to compare DING engine technology with various other engine technologies in a number of commercial applications. The model shows the most likely commercial applications for DING technology and can also be used to identify the sensitivity of variables that impact commercial viability. (4) MVE, Inc., completed a preliminary design concept study that examines the major design issues involved in making a reliable and durable 3,000 psi LNG pump. A primary concern is …

The primary goal for the impurity tests performed at SRS was to determine the maximum level of volatile impurities that can be accommodated into the ceramic form without significantly affecting product properties. The properties investigated in this study are the apparent porosity and the phase assemblage.

This project will examine the chemical speciation of neptunium in spent nuclear fuel. The R&D fields covered by the project include waste host materials and actinide chemistry. Examination of neptunium is chosen since it was identified as a radionuclide of concern by the NERI workshop. Additionally, information on the chemical form of neptunium in spent fuel is lacking. The identification of the neptunium species in spent fuel would allow a greater scientific based understanding of its long-term fate and behavior in waste forms. Research to establish the application and development of X-ray synchrotrons radiation (XSR) techniques to determine the structure of aqueous, adsorbed, and solid actinide species of importance to nuclear considerations is being conducted at Argonne. These studies extend current efforts within the Chemical Technology Division at Argonne National Laboratory to investigate actinide speciation with more conventional spectroscopic and solids characterization (e.g. SEM, TEM, and XRD) methods. Our project will utilize all these techniques for determining neptunium speciation in spent fuel. We intend to determine the chemical species and oxidation state of neptunium in spent fuel and alteration phases. Different types of spent fuel will be examined. Once characterized, the chemical behavior of the identified neptunium species will be …

Sandia National Laboratories has developed a computer based model called IVSEM (Integrated Verification System Evaluation Model) to estimate the performance of a nuclear detonation monitoring system. The IVSEM project was initiated in June 1994, by Sandia's Monitoring Systems and Technology Center and has been funded by the U.S. Department of Energy's Office of Nonproliferation and National Security (DOE/NN). IVSEM is a simple, ''top-level,'' modeling tool which estimates the performance of a Comprehensive Nuclear Test Ban Treaty (CTBT) monitoring system and can help explore the impact of various sensor system concepts and technology advancements on CTBT monitoring. One of IVSEM's unique features is that it integrates results from the various CTBT sensor technologies (seismic, in sound, radionuclide, and hydroacoustic) and allows the user to investigate synergy among the technologies. Specifically, IVSEM estimates the detection effectiveness (probability of detection), location accuracy, and identification capability of the integrated system and of each technology subsystem individually. The model attempts to accurately estimate the monitoring system's performance at medium interfaces (air-land, air-water) and for some evasive testing methods such as seismic decoupling. The original IVSEM report, CTBT Integrated Verification System Evaluation Model, SAND97-25 18, described version 1.2 of IVSEM. This report describes the changes made …

The purpose of this waste analysis plan (WAP) is to document the waste acceptance process, sampling methodologies, analytical techniques, and overall processes that are undertaken for waste accepted for storage and/or disposal at the Low-Level Burial Grounds which are located in the 200 East and West Areas of the Hanford Facility, Richland, Washington. This WAP documents the methods used to characterize, obtain and analyze representative samples of waste managed at this unit.

Canceled see HNF-5841 Rev 0. This waste analysis plan (WAP) has been prepared for the Low-Level Burial Grounds which are located in the 200 East and West Areas of the Hanford Facility, Richland, Washington. This WAP documents the methods used to characterize, obtain and analyze representative samples of waste managed at this unit.

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Debt Swapping as a Tool for Economic and Social Stablization in Russia's Closed Nuclear Cities

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Particulate emissions from spark-ignited (SI) engines have come under close scrutiny as they tend to be smaller than 50 nm, are composed mainly of volatile organic compounds, and are emitted in significant numbers. To assess the impact of such emissions, measurements were performed in the exhaust of a current-technology port-fuel-injected SI engine, which was operated at various steady-state conditions. To gain further insights into the particulate formation mechanisms, measurements were also performed upstream of the catalytic converter. At all engine speeds, a general trend was observed in the number densities and mass concentrations: a moderate increase at low loads followed by a decrease at mid-range loads, which was followed by a steep increase at high loads. Within reasonable bounds, one could attribute such a trend to three different mechanisms. An unidentified mechanism at low loads results in particulate emissions monotonically increasing with load. At medium loads, wherein the engine operates close to stoichiometric conditions, high exhaust temperatures lead to particulate oxidation. At high loads, combustion occurs mostly under fuel-rich conditions, and the contribution from combustion soot becomes significant. Estimates of the number of particles emitted per kilometer by a vehicle carrying the current test engine were found to be lower …

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In August 1999, EnerTech Environmental, LLC (EnerTech) and the Federal Energy Technology Center (FETC) entered into a Cooperative Agreement to develop the first SlurryCarb{trademark} facility for converting Municipal Sewage Sludge (MSS) into a high-density slurry fuel, which could be co-utilized with coal in various industrial applications. Funded primarily by private investors, this program was divided into two major phases, Project Definition (Phase 0) and Design, Construction, and Operation (Phase 1). Project Definition, performed during this reporting period, was designed to define the project from a technical, economic, and scheduling standpoint. Once defined, much of the project risk would be appropriately mitigated thereby providing stakeholders, such as FETC, less risk when investing in the more costly Phase 1, which includes the design, construction, and operation of the first SlurryCarb{trademark} facility. Since May 1999, EnerTech has made significant progress in the tasks required in Phase 0 for bringing this project to Phase 1. These accomplishments have enhanced the probability for success thereby reducing the risk to the United States Department of Energy's (DOE) for its investment in the project. Phase 0 technical accomplishments include: Locating and securing a project site for the 60 dry ton per day (DTPD) SlurryCarb{trademark} facility; Locating and …

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