We have been developing a high throughput 454 library construction process at the Joint Genome Institute to meet the needs of de novo sequencing a large number of microbial and eukaryote genomes, EST, and metagenome projects. We have been focusing efforts in three areas: (1) modifying the current process to allow the construction of 454 standard libraries on a 96-well format; (2) developing a robotic platform to perform the 454 library construction; and (3) designing molecular barcodes to allow pooling and sorting of many different samples. In the development of a high throughput process to scale up the number of libraries by adapting the process to a 96-well plate format, the key process change involves the replacement of gel electrophoresis for size selection with Solid Phase Reversible Immobilization (SPRI) beads. Although the standard deviation of the insert sizes increases, the overall quality sequence and distribution of the reads in the genome has not changed. The manual process of constructing 454 shotgun libraries on 96-well plates is a time-consuming, labor-intensive, and ergonomically hazardous process; we have been experimenting to program a BioMek robot to perform the library construction. This will not only enable library construction to be completed in a single …

This report discusses the Servicemembers Civil Relief Act, which seeks to provide for, strengthen, and expedite the national defense by protecting servicemembers, enabling them to "devote their entire energy to the defense needs of the Nation." The SCRA protects servicemembers by temporarily suspending certain judicial and administrative proceedings and transactions that may adversely affect their legal rights during military service.

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Daily newspaper from Sweetwater, Texas that includes local, state, and national news along with advertising.

This report covers issues for Congress regarding text and multimedia messaging. The increasing use of text and multimedia messaging has raised several policy issue, such as distracted driving, SMS spam, the inability of consumers to disable text messaging, text messaging price fixing, carrier blocking of common short code messages, deceptive and misleading common short code programs, protecting children from inappropriate content on wireless devices.

This report describes several pension benefit programs for veterans and their families, including the eligibility criteria and current benefit levels.