This effort has undertaken the task of developing a link between the genomics, DNA repair and structural biology efforts within the Biology and Biotechnology Research Program at LLNL. Through the advent of the I.M.A.G.E. (Integrated Molecular Analysis of Genomes and their Expression) Consortium, a world-wide effort to catalog the largest public collection of genes, accepted and maintained within BBRP, it is now possible to systematically express the protein complement of these to further elucidate novel gene function and structure. The work has ensued in four phases, outlined as follows: (1) Gene and System selection; (2) Protein expression and purification; (3) Structural analysis; and (4) biological integration. Proteins to be expressed have been those of high programmatic interest. This includes, in particular, proteins involved in the maintenance of genome integrity, particularly those involved in the repair of DNA damage, including ERCC1, ERCC4, XRCC2, XRCC3, XRCC9, HEX1, APN1, p53, RAD51B, RAD51C, and RAD51. Full-length cDNA cognates of selected genes were isolated, and cloned into baculovirus-based expression vectors. The baculoviral expression system for protein over-expression is now well-established in the Albala laboratory. Procedures have been successfully optimized for full-length cDNA clining into expression vectors for protein expression from recombinant constructs. This includes the …

The over-reaching goal of the Groundwater Grand Challenge component of the Partnership in Computational Science (PICS) was to develop and establish the massively parallel approach for the description of groundwater flow and transport and to address the problem of uncertainties in the data and its interpretation. This necessitated the development of innovative algorithms and the implementation of massively parallel computational tools to provide a suite of simulators for groundwater flow and transport in heterogeneous media. This report summarizes the activities and deliverables of the Princeton University component of the Groundwater Grand Challenge project funded through the High Performance Computing grand challenge program of the Department of Energy from 1995 through 1998. Seven institutions were primarily involved in this project: Brookhaven National Laboratory, Oak Ridge National Laboratory, Princeton University, SUNY at Stony Brook, Texas A&M University, The University of South Carolina, and the University of Texas at Austin, with contributing efforts from the Westinghouse Savannah River Technology Center. Each institution had primary responsibility for specific research components, but strong collaboration among all institutions was essential for the success of the project and in producing the final deliverables. PICS deliverables include source code for the suite of research simulators and auxiliary HPC …

This report is a guide to appropriations of Legislative Branch.

Appropriations are one part of a complex federal budget process that includes budget resolutions, appropriations (regular, supplemental, and continuing) bills, rescissions, and budget reconciliation bills. This report is a guide to one of the 13 regular appropriations bills that Congress passes each year. It is designed to supplement the information provided by the House and Senate Appropriations Subcommittees on Legislative Branch Appropriations.

This report discusses recent congressionally mandated reductions in the Department of Defense(DOD) acquisition workforce.

The objective of this manual is to present guidelines and procedures for the preparation of new data for the Tertiary Oil Recovery Information System (TORIS) data base. TORIS is an analytical system currently maintained by the Department of Energy's (DOE) Bartlesville Project Office. It uses an extensive field- and reservoir-level data base to evaluate the technical and economic recovery potential of specific crude oil reservoirs.

Residual, or unburned carbon in fly ash affects many aspects of power plant performance and economy including boiler efficiency, electrostatic precipitator operation, and ash as a salable byproduct. There is a large concern in industry on the unburned carbon problem due to a variety of factors, including low-NOx combustion system and internationalization of the coal market. In recent work, it has been found that residual carbon extracted from fly ash is much less reactive than the laboratory chars on which the current kinetics are based. It has been suggested that thermal deactivation at the peak temperature in combustion is a likely phenomenon and that the structural ordering is one key mechanism. The general phenomenon of carbon thermal annealing is well known, but there is a critical need for more data on the temperature and time scale of interest to combustion. In addition, high resolution transmission electron microscopy (HRTEM) fringe imaging, which provides a wealth of information on the nature and degree of crystallinity in carbon materials such as coal chars, has become available. Motivated by these new developments, this University Coal Research project has been initiated with the following goals: to determine transient, high-temperature, thermal deactivation kinetics as a function …

The stated goal of this project was to supply The Institute for Genomic Research (TIGR) with pure DNA from the bacterium Deinocmus radiodurans RI for purposes of complete genomic sequencing by TIGR. We subsequently decided to expand this project to include a second goal; this second goal was the development of a NotI chromosomal map of D. radiodurans R1 using Pulsed Field Gel Electrophoresis (PFGE).

This report summarizes the results of cold testing, completed by AEAT, as part of the proof-of-principle testing for a proposed nested, fixed-depth fluidic sampling system. This sampling system will provide waste samples from the PHMC feed tank to support the privatization contract with BNFL. Proof-of-principle tests were completed with 2 wt% and 10 wt% sand/water and 25 wt% kaolin clay/water simulants with a test setup that spanned the 24 ft to 57 ft height required in the feed tank. The tests demonstrated that the system could pump and sample waste materials with low and with high solids content. In addition, the tests demonstrated a need for some design upgrades to the sampling system, as there was material loss when the sample bottle was removed from the sampling needle. These were complementary tests, completed as part of an EM-50 Tank Focus Area (TFA) to develop a sampling system for validating LAW and HLW waste batches for the Privatization Contract.

This is the final year of the 3-year LDRD-ERD involving Lasers, D&NT, Physics, and ILSA to develope astrophysics experiments on intense lasers such as the Nova and Gekko lasers. During this 3 year period, we have developed a highly successful experiment probing the hydrodynamics of the explosion phase of core-collapse supernovae, which occurs during the first ~3 hours after core collapse. This was in collaboration with the Univ. of Arizona and CEA/Saclay. We also developed a very successful experiment to probe the hydrodynamics of the later time, young remnant phase, meaning the first ~10-20 years after core collapse. This was in collaboration with the Univ. of Michigan and Univ. of Colorado. Finally, we developed during the final year an exquisite experiment to probe the dynamics of radiative, high Mach number astrophysical jets, in collaboration with the Univ. of Maryland and Osaka Univ. Each experiment has received very high visibility, with a multitude of publications, both in the technical journals (most importantly, the astrophysical journals) and in the popular press. The attached publication list shows 25 papers published or submitted to technical journals, 5 articles appearing in the popular press (including a cover story of Sky and Telescope), and 65 conference …

The focus of this report is on the results related to evaluation of in situ combustion processes applied to Schrader Bluff. Initially, overall screening processes were applied to determine which of the EOR methods, were most appropriate for Schrader Bluff. In situ combustion was among the methods considered potentially favorable and was evaluated further. Laboratory scale tube runs were conducted to determine if the kinetic parameters for the crude oil were favorable. Additional sensitivity studies were conducted to evaluate the recovery potential. Described in this report are the results of the (1) initial screening,(2) experimental tube runs, and (3) simulation sensitivity studies as related to in situ combustion in Schrader Bluff.

Gravanom provides you with a simple, handy tool for modeling gravity data from 2-D geological structures on a personal computer.

Maganom is a computer program for modeling magnetic data over 2-D structures. The program computes the magnetic anomalies across 2-D structures (models) to allow you to compare observed and computed magnetic data across the model structure. If a match between the computed and the observed magnetic values is unsatisfactory, you construct a new model and rerun Maganom to recalculate new magnetic values. In this way, you can continue calculations until you obtain a satisfactory match between the observed and the calculated values.

In the second half of calendar year 1998, no work was performed on the present project. The 20 MMBtu/hr combustor-boiler facility was operated for 11 tests, primarily with Coal Tech resources on biomass combustion and gasification. The total test days on the Philadelphia facility to the end of August 1998 was 119. Of these, 36 tests were part of another DOE project on sulfur retention is slag, and 8 were on an in-house biomass combustion effort. The test days on the other project are listed here because they demonstrate the durability of the combustor, which is one of the objectives of the present project. Also, the test work of 1998 revealed for the first time the major potential of this combustor for biomass combustion. These tests are double the 63 tests in the original plan for this project. All key project objectives have been exceeded including combustor durability, automated combustor operation, NO{sub x} emissions as low as 0.07 lb/MMBtu and SO{sub 2} emissions as low as 0.2 lb/MMBtu. In addition, a novel post-combustion NOx control process has been tested on a 37 MW and 100 MW utility boiler. The only effort remaining on this project is facility disassembly and Final …

This report summarizes the work accomplished in the authors laboratories over the previous three years. During the funding period they have had 23 monographs published or in press, 1 book chapter, 1 patent issued and have delivered 28 invited seminars or plenary lectures on DOE sponsored research. This report covers the work that has been published (or accepted). The most notable aspect of this work involves the successful development and understanding of a new class of fused macrocyclic compounds as pseudophases and selectors in high performance separations (including high performance liquid chromatography, HPLC; capillary electrophoresis, CE; and thin layer chromatography, TLC). They have considerably extended their chiral biomarker work from amber to crude oil and coal. In the process of doing this we've developed several novel separation approaches. They finished their work on the new GSC-PLOT column which is now being used by researchers world-wide for the analysis of gases, light hydrocarbons and halocarbons. Finally, we completed basic studies on immobilizing a cyclodextrin/oligosiloxane hybrid on the wall of fused silica, as well as a basic study on the separation behavior of buckminster fullerene and higher fullerenes.