This study investigates (1) whether infants in a day-care setting exhibit sexual preferences in the choice of a playmate, and (2) whether males exhibit more overt acts in play than do females. Eight male and eight female infants, attending a day-care center, paired by age (ages twelve to twenty-four months), were selected as subjects. Each of the sixteen children was observed for a ten-minute period on four separate days, over a two-week period, a total of forty minutes' observation time per child. No significant differences were found between male and female infants involving the preference of the sex of a playmate, or between male and female overt behaviors.