Currently, the public has a strong sense of the need for environment protection and the use of sustainable, or “green,” design in buildings and other civil structures. Since green design elements and technologies are different from traditional design, they probably have impacts on the building environment, such as vibration, lighting, noise, temperature, relative humidity, and overall comfort. Determining these impacts of green design on building environments is the primary objective of this study. The Zero Energy Research (ZOE) laboratory, located at the University of North Texas Discovery Park, is analyzed as a case study. Because the ZOE lab is a building that combines various green design elements and energy efficient technologies, such as solar panels, a geothermal heating system, and wind turbines, it provides an ideal case to study. Through field measurements and a questionnaire survey of regular occupants of the ZOE lab, this thesis analyzed and reported: 1) whether green design elements changed the building’s ability to meet common building environmental standards, 2) whether green design elements assisted in Leadership in Energy and Environmental Design (LEED) scoring, and 3) whether green design elements decreased the subjective comfort level of the occupants.