Data management plan for the grant, "Fatty Acid Amide Hydrolases and Chemical Communication in Plants." Research seeking to understand how an evolutionarily-conserved group of plant enzymes utilizes chemical signals to regulate growth and to influence their microbial environment. Specific research aims include the discovery of new enzymes and their substrate molecules that act as communication signals. Broader applications of this research may offer new strategies to enhance agricultural outputs by manipulating plant-microbe interactions in crop and soil systems.

Data management plan for the grant "IRES Track II - Cape Horn ASIs: Climate change and disease ecology at the southern end of the Americas." Research giving students the opportunity to be trained in quantitative experimental design and work as part of a multinational research collaboration to study infectious disease emergence in one of the few remaining pristine places on Earth. The Cape Horn Biosphere Reserve (CHBR) off the southern tip of South America protects pristine ecosystem is perfect for this type of graduate student training. The CHBR is part of the sub-Antarctic Magellanic ecoregion, which is globally significant because it houses the worlds southernmost forest biome, contains numerous endemic species, is remote, and is relatively free of anthropogenic impacts. The research questions themselves will be flexible, but organized thematically. Specifically, this IRES Track-II will focus on the merging molecular genetic analysis using a mobile next generation sequencing lab with mist netting and arthropod trapping to investigate the impacts of wildlife disease on local biodiversity and community structure. Secondarily, eDNA and traditional wildlife disease monitoring approaches will be applied to understand the potential for zoonosis and understanding ecological factors that contribute to, or inhibit, zoonosis. Resultantly, participation in this program …

This article uses currently available software and techniques to describe a workflow to analyze ¹³C-labeled isotopologues of the membrane lipid and storage oil lipid intermediate—phosphatidylcholine (PC). Results demonstrated greater ¹³C-isotopic labeling in the cotyledons of developing embryos compared with the embryonic axis.

This article identifies discriminant metabolites between a resistant (R) and a susceptible (S) sugarcane variety at the early stages of pathogen colonization (30 and 120 days after inoculation—DAI) by untargeted and targeted metabolomics of leaves and xylem sap using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The data represent a valuable resource of potential biomarkers for metabolite-assisted selection of resistant varieties to RS.

This article summarizes the best available information about managing ecological corridor systems. The aim with is to provide managers with a convenient guidance document and tool to assist in applying scientific management principles to management of corridors. It does not cover issues related to corridor design or political buy in, but focuses on how a corridor should be managed once it has been established.

Article describing the anatomy of the trunk muscles, their properties that determine muscle performance (mass, length and physiological cross-sectional area [PCSA]) and investigating their scaling in juvenile Alligator mississippiensis spanning an order of magnitude in body mass (359 g–5.5 kg). Findings support existing anecdotal evidence that crocodilians shift their breathing mechanics as they increase in size.

This article uses metazachlor, an inhibitor of VLCFA synthesis, to find that VLCFAs are involved in the TGN/MVB distribution of PI3P. This effect is independent from either synthesis of PI3P by PI3-kinase or degradation of PI(3,5)P2 into PI3P by the SUPPRESSOR OF ACTIN1 (SAC1) phosphatase. Results suggest that PI3P might be transferable from TGN to MVBs and that VLCFAs act in this process.

This article is a review presenting examples of gene editing responsible for conferring both biotic and abiotic stresses in tomato simultaneously. The literature on using this powerful technology to improve fruit quality, yield, and nutritional aspects in tomato is highlighted.

This article identifies, in Arabidopsis, an oxalyl-CoA decarboxylase (AtOXC) that is capable of catalyzing the second step in the proposed pathway of oxalate catabolism.

This article is a dataset that describes behavioral results in zebrafish (Danio rerio) individually exposed to methanol, a solvent which is capable of altering physiology and behavior high concentrations.

Article exploring the association between second-hand smoke exposure, asthma, and asthma severity in children ages 6-17 as well as the effect of birth weight and prematurity (BWP) on parental smoking and asthma. Results indicate that focused asthma interventions in children should inquire about BWP status as well as parental smoking and household smoke exposure to reduce asthma morbidity and mortality.

This article's aim is to determine whether (a) the spatial use of domestic dogs increases with habitat destruction, and (b) domestic dogs and habitat destruction drive the spatial use of native carnivores in a heavily degraded agricultural landscape. Conclusions highlight that domestic dog occupancy was positively correlated with habitat loss and future conditions of increased fragmentation and habitat loss will likely increase the potential contact between domestic dogs and native carnivores.

This article evaluates the role of endozoochory in herbivorous and sympatric bird species in sub-Antarctic Chile. The authors hypothesize that fragments of bryophyte gametophytes retrieved from their feces are viable and capable of regenerating new plant tissue. This relationship may have important implications in the way bryophytes disperse and colonize habitats facing climate change.

This article explores DNA methylation's role in the regulation of gene expression in reptiles. Results show that embryonic hypoxia programs DNA methylation and gene expression patterns and that these changes are associated with enhanced cardiac anoxia tolerance in snapping turtles later in life.

Article using decadal datasets that span aquatic and terrestrial macrosystems and structural equation modeling to show that local temporal variability and spatial synchrony increase temporal variability for entire macrosystems. This analysis is among the first to provide a quantitative argument for the value of regional species diversity.

This article includes a proteomics analysis of Lipid Droplets (LDs) isolated from drought-stressed Arabidopsis leaves and identifying EARLY RESPONSIVE TO DEHYDRATION 7 (ERD7) as a putative LD protein. Observations provide new insight to ERD7 and the SD-containing family of proteins in plants and suggest that ERD7 may be involved in functional aspects of plant stress response that also include localization to the LD surface.

Article utilizing Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations in the FATTY ACID DESATURASE2 (FAD2) and REDUCED OLEATE DESATURATION1 (ROD1) genes to increase oleic acid content in Pennycress (Thlaspi arvense L.). Results suggest that combinatorial knockout of ROD1 and FAE1 may be a viable route to commercially increase oleic acid content in pennycress seed oil whereas mutations in FAD2 will likely require at least partial function to avoid fitness trade-offs.

Article studying the king quail as an animal model to determine if chronic dietary exposure to crude oil in a parental population affects morpho-physiological phenotypic variables in their immediate offspring generation.

This article examines the relationships between spatial patterns of long-term ozone exposure and respiratory illness within Dallas-Fort Worth to better understand impacts on health outcomes.

Data management plan for the grant, "NSFDEB-NERC: Collaborative Research: Wildlife corridors: do they work and who benefits?" Research on the impact of wildlife corridors using genetics as the measure of effectiveness. The study will use 20 independent landscapes to quantify how corridor traits affect gene flow, and will use non-flying mammals as focal species because they are strongly affected by fragmentation. The research team hypothesizes (1) a strong non-linear decline in success (gene flow) with corridor length, reflecting the skewed distribution of dispersal distances within species; (2) success will drop steeply as corridor width falls below a threshold, with the threshold determined by species traits; and (3) species that are bigger, are habitat specialists, or have greater dispersal abilities (relative to brain size or reproductive rate) will benefit more from corridors. Testing these hypotheses will allow generalization to a wide range of mammal species not included in this project. It will use highly flexible Random Forest models to answer the overarching question: What landscape traits (e.g., corridor width, degree of human disturbance) and species traits (mobility, affinity to particular land cover types) are associated with effective corridors?

Data management plan for the grant "G-RISE at the University of North Texas." The University of North Texas (UNT) serves over 32,000 undergraduate and 7,000 graduate students of which 50% of the undergraduate population and 20% of the graduate population are underrepresented minority (URM). UNT is ranked by the Carnegie Classification as a Tier 1 institute, is a Hispanic Serving Institute, and is dedicated to providing quality mentoring to a diverse group of Ph.D. students. The UNT G-­RISE mission is to provide a biomedical Ph.D. training program that is inclusive, culturally responsive, increases diversity, enhances scientific skill sets, develops trainee career and professional skill sets, provides opportunities to participants, and increases faculty development as a mentor.

This article analyzes the transcriptional control of PA and anthocyanin biosynthesis in soybean. Results show that engineered PA biosynthesis in soybean exhibits qualitative and spatial differences from the better-studied model systems Arabidopsis thaliana and Medicago truncatula, and suggest targets for engineering PAs in soybean plants.

This article investigates the hypothesis that exposure to engine emissions increases systemic and local adipocyte RAS signaling, promoting the expression of factors involved in cardiovascular disease and obesity.

This article generates transgenic cotton lines expressing the barley (Hordeum vulgare) homogentisate geranylgeranyl transferase coding sequence under the control of the Brassica napus seed-specific promoter, napin. Findings may provide new opportunities for cottonseed co-products with enhanced vitamin E profile for improved shelf life and nutrition.