Several hybridization-based methods used to delineate single copy or repeated DNA sequences in larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitative DNA fiber mapping (QDFM) differs from the majority of these methods in that it applies FISH to purified, clonal DNA molecules which have been bound with at least one end to a solid substrate. The DNA molecules are then stretched by the action of a receding meniscus at the water-air interface resulting in DNA molecules stretched homogeneously to about 2.3 kb/{micro}m. When non-isotopically, multicolor-labeled probes are hybridized to these stretched DNA fibers, their respective binding sites are visualized in the fluorescence microscope, their relative distance can be measured and converted into kilobase pairs (kb). The QDFM technique has found useful applications ranging from the detection and delineation of deletions or overlap between linked clones to the construction of high-resolution physical maps to studies of stalled DNA replication and transcription.

Evaporitic environments are found in a variety of depositional environments as early as the Archean. The depositional settings, microbial community and mineralogical composition vary significantly as no two settings are identical. The common thread linking all of the settings is that evaporation exceeds precipitation resulting in elevated concentrations of cations and anions that are higher than in oceanic systems. The Dead Sea and Storrs Lake are examples of two diverse modern evaporitic settings as the former is below sea level and the latter is a coastal lake on an island in the Caribbean. Each system varies in water chemistry as the Dead Sea dissolved ions originate from surface weathered materials, springs, and aquifers while Storrs Lake dissolved ion concentration is primarily derived from sea water. Consequently some of the ions, i.e., Sr, Ba are found at significantly lower concentrations in Storrs Lake than in the Dead Sea. The origin of the dissolved ions are ultimately responsible for the pH of each system, alkaline versus mildly acidic. Each system exhibits unique biogeochemical properties as the extreme environments select certain microorganisms. Storrs Lake possesses significant biofilms and stromatolitic deposits and the alkalinity varies depending on rainfall and storm activity. The microbial community …

Discusses "radio communication fundamentals and the methods and technique involved in the installation and operation of radio communication equipment."

The Himalayas and the Tibetan Plateau were formed as a result of the collision of India and Asia, and provide an excellent opportunity to study the mechanical response of the continental lithosphere to tectonic stress. Geophysicists are divided in their views on the nature of this response advocating either (1) homogeneously distributed deformation with the lithosphere deforming as a fluid continuum or (2) deformation is highly localized with the lithosphere that deforms as a system of blocks. The resolution of this issue has broad implications for understanding the tectonic response of continental lithosphere in general. Homogeneous deformation is supported by relatively low decadal, geodetic slip-rate estimates for the Altyn Tagh and Karakorum Faults. Localized deformation is supported by high millennial, geomorphic slip-rates constrained by both cosmogenic and radiocarbon dating on these faults. Based upon the agreement of rates determined by radiocarbon and cosmogenic dating, the overall linearity of offset versus age correlations, and on the plateau-wide correlation of landscape evolution and climate history, the disparity between geomorphic and geodetic slip-rate determinations is unlikely to be due to the effects of surface erosion on the cosmogenic age determinations. Similarly, based upon the consistency of slip-rates over various observation intervals, secular variations …

This strategic plan is in direct response to the call by a broad array of interested parties, for the Office of Building Technology, State and Community Programs (BTS) to reduce fragmentation and increase focus. This plan outlines goals for saving energy, three key strategies to accomplish these goals, and a commitment to improving how they do business.

The January 1999 issue of Energy Matters (formerly Turning Point) focuses on utility deregulation and restructuring and the implications for industry. An article describes how the Northeast Premium Efficiency Motor Initiative plans to increase the availability of premium efficiency motors. A case study reveals how General Motors achieved energy and cost savings by installing an energy-efficient water booster pumping system as part of a facility renovation.

Interview with Betty Andujar, a Republican member of the Texas Senate from Fort Worth, Texas, discussing her political career and experiences as a member of the 67th Texas Legislature. Andujar provides commentary on the gubernatorial elections, initiative-referendum, the state water plan, taxes, and new bills.

Interview with Archie Wilkerson, a realtor and a Navy veteran, concerning his experiences at Ford Island Naval Air Station with VP-23 during the Japanese attack at Pearl Harbor on December 7, 1941.