Seal Pump Outs (PO) are provided by specifically designing and providing redundant, or double, seals to create an annular volume that can be '(vacuum) pumped out' to test the integrity of both seals. The value of the technique is most readily apparent in the construction of large piping systems or vessels whose closure is on a different schedule than the nozzle closures, or whose nozzles are serially closed. In the case of D0, for instance, the high voltage boxes were put in place and leak checked before the vessel was closed and independent of the other nozzles. PO use is by no means limited to cryogenics and the supporting vacuum systems, but the discussion here will be limited to cryogenic applications. POs come in two generic service types; installation, and installation and monitor. The above high voltage box is an example of a static installation service application. Once the item is installed the PO can be, and almost universally is, capped, and revisited only on disassembly and reassembly. POs are constantly monitored after installation only when their seals, through cooldown gradient induced motion, vibration, cyclic load bolt seating, or other dynamic phenomena may degrade in performance over time. PO seals …

None

The project was designed to develop and apply a novel unconventional approach to genome mapping based on physical properties of DNA that are a sensitive function of the base sequence, and so does not depend on the clonability of the sequences to be mapped nor on the presence of particular restriction sites. We have shown that a broad array of DNA fragments are retarded at nearly the same level in denaturing gradient gel electrophoresis (DGGE) if the segment with the lowest thermal stability has the same melting temperature, regardless of the length of the fragment. The retarded pattern remain steady in the gel, changing little with continued field exposure. Mapping proceeds by the analysis of two-dimensional patterns produced by random fragmentation of genomic DNA and denaturing gradient gel electrophoresis. Random fragments are first separated according to length by conventional agarose electrophoresis. The result is a two- dimensional pattern which can be idealized as an array of nearly parallel, mostly separated lines of DNA. The pattern is blotted onto a membrane and probed sequentially with oligos or relevant DNA or RNA fragments. The endpoints on the fragment length scale of each line hybridizing with each probe, the distribution along each line, …

None

This report examines aviation, transit and passenger rail security, as well as port and maritime security. The difficulty and cost of protecting the transportation sector from attack raises a core question for policymakers: how much effort and resources to put toward protecting potential targets versus pursuing and fighting terrorists.

This report provides background information and presents potential issues (and costs) for Congress concerning the U.S. Navy's ship force-structure goals and shipbuilding plans.

Experiments will mainly focus on breaking the bonds within the slag itself using detonation wave. For the experiments, initial suggestion was to build up slag deposit around a representative tube by placing it inside the convection pass of an actual boiler at the Northern States Power Company. But it was later concluded that once the tube is cooled to room condition, the thermal stress will greatly reduce the bonding between the heat transfer surface and the slag. It was concluded that the slag will be attached to the tube using high density epoxy resin. High density epoxy will be used so that they do not diffuse into the slag and strengthen the bonding within the slag. Suggestions on candidate epoxy are provided by MTI lab. MTI also provided PVAMU with different kinds of slags for testing. The deposits for characterization were from a subbituminous coal fired utility boiler.