A chemiluminescent homogeneous immunoassay and a hand-size multiassay reader are described that could be used for detecting biological materials. The special feature of the assay is that it employs two different antibodies that each bind to a unique epitope on the same antigen. Each group of epitope-specific antibodies has linked to it an enzyme of a proximal-enzyme pair. One enzyme of the pair utilizes a substrate in high concentration to produce a second substrate required by the second enzyme. This new substrate enables the second enzyme to function. The reaction of the second enzyme is configured to produce light. This chemiluminescence is detected with a charge-coupled device (CCD) camera. The proximal pair enzymes must be in close proximity to one another to allow the second enzyme to react with the product of the first enzyme. This only occurs when the enzyme-linked antibodies are attached to the antigen, whether antigen is a single protein with multiple epitopes or the surface of a cell with a variety of different antigens. As a result of their juxtaposition, the enzymes produce light only in the presence of the biological material. A brief description is given as to how this assay could be utilized in …

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This report describes the RCRA assessment plan for single-shell tank Waste Management Area S-SX

The following ceramics were prepared for this study: the baseline ceramic, the baseline ceramic plus process impurities, a zirconolite-rich composition, a brannerite-rich composition, a composition designed to have {approx} 10% perovskite in addition to the normal baseline phases, and an {approx} 10% phosphate-doped batch. These samples were prepared by oxide (via wet or dry milling) and alkoxide-routes. The milling method has a direct effect on the samples. Incomplete milling leads to inhomogeneity in the microstructure. The main effect of incomplete milling is that unreacted actinide oxides remain in the microstructure, usually surrounded by brannerite. The phase composition also alters due to the actinide being tied up in the unreacted oxide, e.g., the pyrochlore has less actinide and additional phases, such as zirconolite may form. The approach to equilibrium is determined by a number of factors--the efficiency of the milling, the sintering time, the sintering temperature and the batch composition. The latter is very important, e.g., the zirconolite-rich batch has a slower approach to equilibrium than the baseline ceramic requiring higher sintering temperatures or longer sintering times. The addition of process impurities dramatically alters the approach to equilibrium. The additives produce a silicate liquid phase (at the sintering temperatures), which aids …

Federal Emergency Management Information System (FEMIS) Bill of Materials (BOM) for FEMIS Version 1.4.6

The purpose of deceleration studies is to construct the deceleration ramp files that are used to decelerate antiproton beam for experiment E835. A deceleration ramp file is a set of ramp tables that are downloaded to PAUX (a part of the Pbar front end) prior to a deceleration. There is a ramp table for each device that is changed during a deceleration. These tables determine how PAUX changes each device setting during a deceleration. Appendix 1 gives a table of all ramped devices. Presently a deceleration to the lowest energy allowed by our ramps requires the use of three ramp files: the first decelerates the beam from 8801 MeV/c to 6367 MeV/c, the second from 6367 MeV/c to 4858 MeV/c, and the third from 4858 MeV/c to 3900 MeV/c. At the time of this writing the third ramp file has yet to be completed. An important new feature of the present deceleration ramp files is that the value of {gamma}{sub t} is decreased as the beam is decelerated so that the Accumulator is always above transition.

The purpose of this calculation is to determine the surface dose rates of a codisposal waste package (WP) containing an intact seed assembly of the Shippingport light-water breeder reactor (LWBR) spent nuclear fuel (SNF) and the Savannah River Site (SRS) high-level waste (HLW) in glass form. The Shippingport LWBR SNF is loaded in a Department of Energy (DOE) standardized 18-in. canister. The canister is surrounded by five 4.5-m-long Hanford pour canisters containing the HLW glass. Gamma dose rate calculation for the WP containing only the HLW glass is also performed. The results will provide information about the contribution of DOE SNF to the total dose rate on the WP surfaces.

The purpose of this calculation is to document that the software routine ModStandardNomal Version 1.0 which is a Visual Fortran 5.0 module, provides correct results for a normal distribution up to five significant figures (three significant figures at the function tails) for a specified range of input parameters. The software routine may be used for quality affecting work. Two types of output are generated in ModStandardNomal: a deviate, x, given a cumulative probability, p, between 0 and 1; and a cumulative probability, p, given a deviate, x, between -8 and 8. This calculation supports Performance Assessment, under Technical Product Development Plan, TDP-EBS-MD-000006 (Attachment I, DIRS 3) and is written in accordance with the AP-3.12Q Calculations procedure (Attachment I, DIRS 4).

This Corrective Action Investigation Plan (CAIP) has been developed in accordance with the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the US Department of Energy, Nevada Operations Office (DOE/NV); the State of Nevada Division of Environmental Protection (NDEP); and the US Department of Defense (FFACO, 1996). The CAIP is a document that provides or references all of the specific information for investigation activities associated with Corrective Action Units (CAUs) or Corrective Action Sites (CASs). According to the FFACO, CASs are sites potentially requiring corrective action(s) and may include solid waste management units or individual disposal or release sites (FFACO, 1996). Corrective Action Units consist of one or more CASs grouped together based on geography, technical similarity, or agency responsibility for the purpose of determining corrective actions. This CAIP contains the environmental sample collection objectives and the criteria for conducting site investigation activities at CAU 135, Area 25 Underground Storage Tanks (USTs), which is located on the Nevada Test Site (NTS). The NTS is approximately 105 kilometers (km) (65 miles [mi]) northwest of Las Vegas, Nevada.

This is the fourth annual report describing the activities performed under Task 1 of Contract No. DE-AC21-94MC31160. The analyses of hot gas stream cleanup (HGCU) ashes and descriptions of filter performance studied under this contract are designed to address problems with filter operation that are apparently linked to characteristics of the collected ash. This work is designed to generate a data base of the key characteristics of ashes collected from operating advanced particle filters and to relate these ash properties to the operation and performance of these filters and their components. This report summarizes characterizations of ash and char samples from pressurized fluidized-bed combustion and gasification facilities. Efforts are under way to develop a method for preserving fragile filter cakes formed on ceramic filter elements. The HGCU data base was formatted for Microsoft Access 97 ® . Plans for the remainder of the project include characterization of additional samples collected during site visits to the Department of Energy / Southern Company Services Power Systems Development Facility and completion and delivery of the HGCU data base.

This is the fourth annual report describing the activities performed under Task 1 of Contract No. DE-AC21-94MC31160. The analyses of hot gas stream cleanup (HGCU) ashes and descriptions of filter performance studied under this contract are designed to address problems with filter operation that are apparently linked to characteristics of the collected ash. This work is designed to generate a data base of the key characteristics of ashes collected from operating advanced particle filters and to relate these ash properties to the operation and performance of these filters and their components. This report summarizes characterizations of ash and char samples from pressurized fluidized-bed combustion and gasification facilities. Efforts are under way to develop a method for preserving fragile filter cakes formed on ceramic filter elements. The HGCU data base was formatted for Microsoft Access 97{reg_sign}. Plans for the remainder of the project include characterization of additional samples collected during site visits to the Department of Energy/Southern Company Services Power Systems Development Facility and completion and delivery of the HGCU data base.

The purpose of this document is to provide a physical, chemical, hazardous and radiological characterization of Low-Level Waste (LLW) generated in HB-Line as required by the 1S Manual, Savannah River Site Waste Acceptance Criteria Manual.

The Ernest Orlando Lawrence Berkeley National Laboratory (LBNL or Berkeley Lab) Laboratory Directed Research and Development Program FY 1998 report is compiled from annual reports submitted by principal investigators following the close of the fiscal year. This report describes the supported projects and summarizes their accomplishments. It constitutes a part of the Laboratory Directed Research and Development (LDRD) program planning and documentation process that includes an annual planning cycle, projection selection, implementation, and review. The LBNL LDRD program is a critical tool for directing the Laboratory's forefront scientific research capabilities toward vital, excellent, and emerging scientific challenges. The program provides the resources for LBNL scientists to make rapid and significant contributions to critical national science and technology problems. The LDRD program also advances LBNL's core competencies, foundations, and scientific capability, and permits exploration of exciting new opportunities. All projects are work in forefront areas of science and technology. Areas eligible for support include the following: Advanced study of hypotheses, concepts, or innovative approaches to scientific or technical problems; Experiments and analyses directed toward ''proof of principle'' or early determination of the utility of new scientific ideas, technical concepts, or devices; and Conception and preliminary technical analyses of experimental facilities or …

The continuity equation for electromagnetic (EM) energy flow is used to describe radiation flow in the transport regime. The scheme of a discrete representation of H_z and a stochastic representation of H_x, H_y gives an effectively continuous representation of directed energy in the x-y plane, such that the ray effect of transport theory is absent. Since the in-plane amplitudes are generated at each time step, it is necessary to store only the perpendicular amplitudes and the results appear to be robust with respect to the coarse of this discretization.

Enhancer of gene silencing 1 (egs1) is an Arabidopsis mutant that enhances post-transcriptional gene silencing of the rolB gene introduced by genetic engineering (transgene). The goal of our proposal was cloning EGS1 based on its map position. Although we screened more than 2000 chromosomes for recombination, we were unable to get closer than 2 cM to the gene. We experienced an unexpected tendency of the post-transcriptionally silenced transgene to switch to a more stable silenced state. This made it impossible to select egs1 homozygotes for map based cloning. This forced us to reconsider our cloning strategy. One possibility would have been to use a different transgene as the target of gene silencing. We tested two other transgenes. Both encoded proteins unrelated to the first but they were all expressed from the same type of promoter and they all had a similar tendency to become post-transcriptionally silenced. After screening over 80 F2 segregants from each cross between our egs1 mutant and Arabidopsis of the same ecotype homozygous for the new transgene, we were disappointed to find that the egs1 mutation did not enhance post-transcription silencing of the two new genes. In 80 plants we expected to have between 4 and 6 …

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This Interface Control Document (ICD) describes the interface between the Double-Shell Tank (DST) System and T Plant. T Plant generates waste during decontamination activities. These facilities do not have a direct physical interface since the waste will be moved by tank trailer to the 204-AR waste unloading facility. The purpose of the ICD process is to formalize working agreements between the River Protection Project (WP) DST System and organization/companies internal and external to RPP. This ICD has been developed as part of the requirements basis for design of the DST System to support the Phase I Privatization effort.

Operational Final Test Report for Integrated Systems, Project W-030 (Phase 2 test, RECIRC and HIGH-HEAT Modes). Project W-030 provides a ventilation upgrade for the four Aging Waste Facility tanks, including upgraded vapor space cooling and filtered venting of tanks AY101, Ay102, AZ101, AZ102.

The Richland Operations Office (RL) and Office of River Protection (ORP) are pleased to issue the attached Pollution Prevention Progress Report. We have just met the most aggressive waste reduction and A recycling goals to date and are publishing this report to recognize A the site's progress, and to ensure it will sustain success beyond 1 Fiscal Year 2000. This report was designed to inform the been made by RL and ORP in Waste Minimization (WMin) and Pollution Prevention (P2). RL, ORP and their contractors are committed to protecting the environment, and we reiterate pollution prevention should continue to be at the forefront of the environmental cleanup and research efforts. As you read the attached report, we believe you will see a clear demonstration of RL and ORP's outstanding performance as it has been responsible and accountable to the nation, its employees, and the community in which we live and work. commitment that all employees have for environmental stewardship. The report provides useful information about the U.S. Department of Energy's (DOE'S) environmental policy and programs, and contains countless examples of waste minimization projects. This year was the first year our site received the White House Closing the Circle in the …

The following description and any attachments and references are provided to the Washington State Department of Health (WDOH), Division of Radiation Protection, Air Emissions & Defense Waste Section as a notice of construction (NOC) in accordance with Washington Administrative Code (WAC) 246-247, Radiation Protection - Air Emissions. WAC 246-247-060, ''Applications, registration, and licensing'', states ''This section describes the information requirements for approval to construct, modify, and operate an emission unit. Any NOC requires the submittal of information listed in Appendix A,'' Appendix A (WAC 246-247-110) lists the requirements that must be addressed. Additionally, the following description, attachments, and references are provided to the U.S. Environmental Protection Agency (EPA) as an NOC, in accordance with Title 40 Code of Federal Regulations (CFR), Part 61, ''National Emission Standards for Hazardous Air Pollutants.'' The information required for submittal to the EPA is specified in 40 CFR 61.07. The potential emissions from this activity are estimated to provide less than 0.1 millirem/year total effective dose equivalent to the hypothetical offsite maximally exposed individual, and commencement is needed within a short time. Therefore, this application also is intended to provide notification of the anticipated date of initial startup in accordance with the requirement listed in …

This Test Plan provides a test method to dedicate the leak detection relays used on the new Pumping and Instrumentation Control (PIC) skids. The new skids are fabricated on-site. The leak detection system is a safety class system per the Authorization Basis.

This sampling and analysis plan (SAP) identifies the type, quantity, and quality of data needed to support characterization of the sludge that remains in Tank 241-2-361. The procedures described in this SAP are based on the results of the 241-2-361 Sludge Characterization Data Quality Objectives (DQO) (BWHC 1999) process for the tank. The primary objectives of this project are to evaluate the contents of Tank 241-2-361 in order to resolve safety and safeguards issues and to assess alternatives for sludge removal and disposal.

This tank sampling and analysis plan (TSAP) identifies the sample collection, laboratory analysis, quality assurance/quality control (QA/QC) objectives for the characterization of catch tank 241-ER-311 vapor space.

This document identifies administrative controls that are established for the operating contractor of the B Plant facility during the B Plant surveillance and maintenance phase. When approved by the DOE-RL, this document is part of the B Plant safety authorization basis. Look for the associated B Plant Surveillance and Maintenance Phase Safety Analysis Report, HNF-3358.