Global changes in gene expression occur during tumor progression, as indicated by expression profiling of metastatic tumors. How this occurs is poorly understood. SATB1 functions as a genome organizer by folding chromatin via tethering multiple genomic loci and recruiting chromatin remodeling enzymes to regulate chromatin structure and expression of a large number of genes. Here we show that SATB1 is expressed at high levels in aggressive breast cancer cells, and is undetectable in non-malignant breast epithelial cells. Importantly, RNAi-mediated removal of SATB1 from highly-aggressive MDA-MB-231 cells altered the expression levels of over 1200 genes, restored breast-like acinar polarity in three-dimensional cultures, and prevented the metastastic phenotype in vivo. Conversely, overexpression of SATB1 in the less-aggressive breast cancer cell line Hs578T altered the gene expression profile and increased metastasis dramatically in vivo. Thus, SATB1 is a global regulator of gene expression in breast cancer cells, directly regulating crucial metastasis-associated genes, including ERRB2 (HER2/NEU), TGF-{beta}1, matrix metalloproteinase 3, and metastasin. The identification of SATB1 as a protein that re-programs chromatin organization and transcription profiles to promote breast cancer metastasis suggests a new model for metastasis and may provide means of therapeutic intervention.

InN nanorods were grown on a, c-, and r-plane of sapphire and also on Si (111) and GaN (0001) by non-catalytic, template-free hydride metal-organic vapor phase epitaxy and studied by transmission electron microscopy, electron energy loss (EELS) and photoluminescence (PL) at room temperature. These nanocrystals have different shapes and different faceting depending on the substrate used and their crystallographic orientation. EELS measurements have confirmed the high purity of these crystals. The observed PL peak was in the range of 0.9-0.95 eV. The strongest PL intensity was observed for the nanocrystals with the larger diameters.

The Generation IV International Forum (GIF) emphasizes proliferation resistance and physical protection (PR&PP) as a main goal for future nuclear energy systems. The GIF PR&PP Working Group has developed a methodology for the evaluation of these systems. As an application of the methodology, Markov model has been developed for the evaluation of proliferation resistance and is demonstrated for a hypothetical Example Sodium Fast Reactor (ESFR) system. This paper presents the case of diversion by the facility owner/operator to obtain material that could be used in a nuclear weapon. The Markov model is applied to evaluate material diversion strategies. The following features of the Markov model are presented here: (1) An effective detection rate has been introduced to account for the implementation of multiple safeguards approaches at a given strategic point; (2) Technical failure to divert material is modeled as intrinsic barriers related to the design of the facility or the properties of the material in the facility; and (3) Concealment to defeat or degrade the performance of safeguards is recognized in the Markov model. Three proliferation risk measures are calculated directly by the Markov model: the detection probability, technical failure probability, and proliferation time. The material type is indicated by …

The need to consider aqueous and sorption kinetics andmicrobiological processes arises in many subsurface problems. Ageneral-rate expression has been implemented into the TOUGHREACTsimulator, which considers multiple mechanisms (pathways) and includesmultiple product, Monod, and inhibition terms. This paper presents aformulation for incorporating kinetic rates among primary species intomass-balance equations. The space discretization used is based on aflexible integral finite difference approach that uses irregular griddingto model bio-geologic structures. A general multi-region model forhydrological transport interacted with microbiological and geochemicalprocesses is proposed. A 1-D reactive transport problem with kineticbiodegradation and sorption was used to test the enhanced simulator,which involves the processes that occur when a pulse of water containingNTA (nitrylotriacetate) and cobalt is injected into a column. The currentsimulation results agree very well with those obtained with othersimulators. The applicability of this general multi-region model wasvalidated by results from a published column experiment ofdenitrification and sulfate reduction. The matches with measured nitrateand sulfate concentrations were adjusted with the interficial areabetween mobile hydrological and immobile biological regions. Resultssuggest that TOUGHREACT can not only be a useful interpretative tool forbiogeochemical experiments, but also can produce insight into processesand parameters of microscopic diffusion and their interplay withbiogeochemical reactions. The geometric- and process-based multi-regionmodel may provide …

The U.S. Support Program (USSP) to IAEA Safeguards priority of destructive analysis is aimed at strengthening the IAEA's ability to use destructive analysis as a safeguards tool. IAEA inspectors bring back nuclear and environmental samples from inspections, which are first cataloged by the IAEA and then analyzed by a network of laboratories located in many Member States and the IAEA's own Safeguards Analytical Laboratory in Seibersdorf, Austria. Historically, the USSP was instrumental in introducing environmental sampling techniques to the IAEA in order to enhance its understanding of material processing activities conducted at nuclear facilities. The USSP has also worked with the IAEA to improve understanding of measurement uncertainty and measurement quality, incorporate new and improved analytical methods, and purchase analytical and computer equipment. Recent activities include a temporary increase in analysis of environmental samples using secondary ion mass spectrometry and provision of a cost-free expert to restore secondary ion mass spectroscopy laboratory functionality and to modernize the IAEA's Safeguards Analytical Laboratory Information System.

Although the emergence of markets for high efficiency off-grid lighting technologies holds promise, realizing the potential of this opportunity on a long-term, sustainable basis requires careful attention to issues of product quality, consumer protection, and the potential for significant 'market spoiling', in anticipation of increases of sales of low cost, low performance off-grid lighting products. The goal of the Lighting Africa quality assurance workshop was to articulate strategies to mitigate the dangers of market spoiling and to explore ways to protect consumers from misleading advertising for sales of inferior, off-grid lighting products in the context of Lighting Africa's overarching objective to support the industry in developing a robust off-grid lighting market in Africa. The workshop resulted in the identification of two strategic approaches for meeting Lighting Africa quality assurance programmatic needs. The first strategy is intended to meet a short-term programmatic need for quality associated with requests for lighting products by bulk procurement agents, such as in a World Bank-financed project. The development of procurement specifications and test procedures that could be used in a quality/usability screening method in order to provide guidance for forthcoming large volume purchases emerged as the best solution to meet this need. Such approaches are …

Recent computations of scattering amplitudes show that N = 8 supergravity is surprisingly well behaved in the ultraviolet and may even be ultraviolet finite in perturbation theory. The novel cancellations necessary for ultraviolet finiteness first appear at one loop in the guise of the ''no-triangle hypothesis''. We study one-loop amplitudes in pure Einstein gravity and point out the existence of cancellations similar to those found previously in N = 8 supergravity. These cancellations go beyond those found in the one-loop effective action. Using unitarity, this suggests that generic theories of quantum gravity based on the Einstein-Hilbert action may be better behaved in the ultraviolet at higher loops than suggested by naive power counting, though without additional (supersymmetric) cancellations they diverge. We comment on future studies that should be performed to support this proposal.

We present simulations and analysis of the heating of warm dense matter foils by ion beams with ion energy less than one MeV per nucleon to target temperatures of order one eV. Simulations were carried out using the multi-physics radiation hydrodynamics code HYDRA and comparisons are made with analysis and the code DPC. We simulate possible targets for a proposed experiment at LBNL (the so-called Neutralized Drift Compression Experiment, NDCXII) for studies of warm dense matter. We compare the dynamics of ideally heated targets, under several assumed equation of states, exploring dynamics in the two-phase (fluid-vapor) regime.

Approach to Connect Global Objectives and Local Actions: (1) Articulate global objectives into a hierarchy of subsystem requirements and local attributes and measures; (2) Establish a baseline system and viable alternatives through the interactions and relationships (e.g., networks) of local system elements and their options; (3) Evaluate performance of system alternatives and develop improved nuclear material management strategies and technologies; and (4) The need to address greatest concerns first (prioritized or graded approach) and to make tradeoffs among implementation options and competing objectives entails a risk-based approach. IGNMM could provide a systematic understanding of global nuclear materials management and evolutionarily improve and integrate the management through an active architecture, using for example, situation awareness, system models, methods, technologies, and international cooperation. Different tools would be used within the overall framework to address individual issues on the desired geographic scale that could be easily linked to broader analyses. Life-cycle system analyses would allow for evaluating material path alternatives on an integrated global scale. Disconnects, overlaps, technical options, and alternatives for optimizing nuclear materials processes could be evaluated in an integrated manner.

Following assessments that attacks with radiological and nuclear weapons are possible, detection system deployments are being supported at national and local levels. Detection systems include both, highly sensitive but non-discriminating detectors, as well as detectors and algorithms capable of distinguishing and identifying gamma rays by energy. The latter systems, usually handheld systems based on sodium iodide detectors, also provide analysis of the specific radionuclides present and are referred to as radioisotope identifiers (RIIDs). Studies have shown that sodium iodide based RIIDs fall far short of 100% accurate identifications. The U.S. Department of Homeland Security (DHS) initiated the Regional Reachback (RRB) Program in 2006 to provide rapid expert interpretation of gamma spectroscopic data from radiation alarms from detection systems deployed by state and local authorities. With expert specialists on call 24/7, RRB provides an avenue for local and state authorities to verify routine results, interpret unknown identifications, and notify national response assets if needed. This paper will provide details of the RRE3 program, an outline of the analysis process, a description of the drills and training systems used to maintain specialists response performance, and examples of drills and incidents from the first full year of operation.

This year's conference on Drosophila research illustratedwell the current focus of Drosophila genomics on the comprehensiveidentification of functional elements in the genome sequence, includingmRNA transcripts arising from multiple alternative start sites and splicesites, a multiplicity of noncoding transcripts and small RNAs,identification of binding sites for transcription factors, sequenceconservation in related species and sequence variation within species.Resources and technologies for genetics and functional genomics aresteadily being improved, including the building of collections oftransposon insertion mutants and hairpin constructs for RNA interference(RNAi). The conference also highlighted progress in the use of genomicinformation by many laboratories to study diverse aspects of biology andmodels of human disease. Here we will review a few highlights of especialinterest to readers of Genome Biology.

Declining natural resources, rising oil prices, looming climate change and the introduction of nuclear energy partnerships, such as GNEP, have reinvigorated global interest in nuclear energy. The convergence of such issues has prompted countries to move ahead quickly to deal with the challenges that lie ahead. However, developing countries, in particular, often lack the domestic infrastructure and public support needed to implement a nuclear energy program in a safe, secure, and nonproliferation-conscious environment. How might countries become ready for nuclear energy? What is needed is a framework for assessing a country's readiness for nuclear energy. This paper suggests that a Nuclear Energy Readiness Indicator (NERI) Index might serve as a meaningful basis for assessing a country's status in terms of progress toward nuclear energy utilization under appropriate conditions. The NERI Index is a benchmarking tool that measures a country's level of 'readiness' for nonproliferation-conscious nuclear energy development. NERI first identifies 8 key indicators that have been recognized by the International Atomic Energy Agency as key nonproliferation and security milestones to achieve prior to establishing a nuclear energy program. It then measures a country's progress in each of these areas on a 1-5 point scale. In doing so NERI illuminates gaps …

The U.S. Support Program to International Atomic Energy Agency (IAEA) Safeguards (USSP) was established in 1977 to transfer US technology and expertise to assist the IAEA Department of Safeguards because its limited budget and scope would not allow for R&D activities and the procurement of specialized or customized equipment. Over the years, the USSP and the Department of Safeguards have worked together continuously to develop and improve processes for requesting, selecting, and managing projects that support the Safeguards verification mission. This paper will discuss the main USSP processes for accepting and processing Safeguards requests, and managing and reporting task progress.

An innovative method for indirectly determining reaction cross sections via Surrogate Nuclear Reactions is presented. Exploring indirect approaches for obtaining reaction cross sections is important since a large number of nuclear reactions relevant to astrophysics cannot be measured with currently available techniques. A program is outlined for developing a comprehensive framework for planning and interpreting experiments that can yield the cross sections of interest. The applications will focus on reactions involving unstable nuclei that play a key role in the production of the elements between iron and uranium.

The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF- 50 binding. The crystal structure of the BARD1 BRCT domain uncovers a degenerate phosphopeptide binding pocket lacking the key arginine required for phosphopeptide interactions in other BRCT proteins.Small angle X-ray scattering together with limited proteolysis results indicates that ankyrin and BRCT domains are linked by a flexible tether and do not adopt a fixed orientation relative to one another. Protein pull-down experiments utilizing a series of purified BARD1 deletion mutants indicate that interactions between the CstF-50 WD-40 domain and BARD1 involve the ankyrin-BRCT linker but do not require ankyrin or BRCT domains. The structural plasticity imparted by the ANK-BRCT linker helps to explain the regulated assembly of different protein BARD1 complexes with distinct functions in DNA damage signaling including BARD1-dependent induction of apoptosis plus p53 stabilization and interactions. BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage …

Universal extra dimensions and supersymmetry have rather similar experimental signatures at hadron colliders. The proper interpretation of an LHC discovery in either case may therefore require further data from a lepton collider. In this paper we identify methods for discriminating between the two scenarios at the linear collider. We study the processes of Kaluza-Klein muon pair production in universal extra dimensions in parallel to smuon pair production in supersymmetry, accounting for the effects of detector resolution, beam-beam interactions and accelerator induced backgrounds. We find that the angular distributions of the final state muons, the energy spectrum of the radiative return photon and the total cross-section measurement are powerful discriminators between the two models. Accurate determination of the particle masses can be obtained both by a study of the momentum spectrum of the final state leptons and by a scan of the particle pair production thresholds. We also calculate the production rates of various Kaluza-Klein particles and discuss the associated signatures.

The purpose of this report is to evaluate the hemodynamic effects of renal vein inflow and filter position on unoccluded and partially occluded IVC filters using three-dimensional computational fluid dynamics. Three-dimensional models of the TrapEase and Gunther Celect IVC filters, spherical thrombi, and an IVC with renal veins were constructed. Hemodynamics of steady-state flow was examined for unoccluded and partially occluded TrapEase and Gunther Celect IVC filters in varying proximity to the renal veins. Flow past the unoccluded filters demonstrated minimal disruption. Natural regions of stagnant/recirculating flow in the IVC are observed superior to the bilateral renal vein inflows, and high flow velocities and elevated shear stresses are observed in the vicinity of renal inflow. Spherical thrombi induce stagnant and/or recirculating flow downstream of the thrombus. Placement of the TrapEase filter in the suprarenal vein position resulted in a large area of low shear stress/stagnant flow within the filter just downstream of thrombus trapped in the upstream trapping position. Filter position with respect to renal vein inflow influences the hemodynamics of filter trapping. Placement of the TrapEase filter in a suprarenal location may be thrombogenic with redundant areas of stagnant/recirculating flow and low shear stress along the caval wall due …

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Washington is called the 'Evergreen State' and it evokes images like this of lush forests, lakes and mountains. However, such images apply primarily to the half of the state west of the Cascade Mountains, where we are today. Eastern Washington state is quite a different matter and I want to draw your attention to a portion of Eastern Washington that is the focus ofmy presentation to you this morning. This image was taken on a part of the Department of Energy's Hanford Site, a 586-square mile government reservation, the second largest DOE facility in the nation . Here you can see where I am talking about, roughly 220 miles southeast of Seattle and about the same distance northeast of Portland.

We implanted ultra low doses (0.2 to 2 x 10{sup 11} cm{sup -2}) of Sb ions into isotopically enriched {sup 28}Si, and probed electrical activation and electron spin relaxation after rapid thermal annealing. Strong segregation of dopants towards both Si{sub 3}N{sub 4} and SiO{sub 2} interfaces limits electrical activation. Pulsed Electron Spin Resonance shows that spin echo decay is sensitive to the dopant profiles, and the interface quality. A spin decoherence time, T{sub 2}, of 1.5 ms is found for profiles peaking 25 nm below a Si/SiO{sub 2} interface, increasing to 2.1 ms when the surface is passivated with hydrogen. These measurements provide benchmark data for the development of devices in which quantum information is encoded in donor electron spins.

Support was provided by DOE for the 2nd ASM Conference on Prokaryotic Development. The final conference program and abstracts book is attached. The conference presentations are organized around topics that are central to the current research areas in prokaryotic development. The program starts with topics that involve relatively simple models systems and ends with systems that are more complex. The topics are: i) the cell cycle, ii) the cytoskeleton, iii) morphogenesis, iv) developmental transcription, v) signaling, vi) multicellularity, and vii) developmental diversity and symbiosis. The best-studied prokaryotic development model systems will be highlighted at the conference through research presentations by leaders in the field. Many of these systems are also model systems of relevance to the DOE mission including carbon sequestration (Bradyrizobium, Synechococcus), energy production (Anabaena, Rhodobacter) and bioremediation (Caulobacter, Mesorhizobium). In addition, many of the highlighted organisms have important practical applications; the actinomycetes and myxobacteria produce antimicrobials that are of commercial interest. It is certain that the cutting-edge science presented at the conference will be applicable to the large group of bacteria relevant to the DOE mission.

More and more articles appear in the press daily about the renaissance of nuclear energy. Even many former opponents of nuclear energy are now convinced that nuclear energy is more environmentally friendly than burning fossil fuels. Nuclear energy does not release carbon dioxide to the atmosphere and therefore does not contribute to the global warming problem. But nuclear energy produces spent fuel or nuclear waste. Spent fuel is radioactive and requires thousands of years of isolation from plants, animals and humans. Every country currently studying the option for disposing of high-level nuclear waste has selected deep geologic formations to be the primary barrier for accomplishing this isolation. It is postulated that by the very nature of these geological sites, they will contain the waste for long time, limiting the spread of radionuclides, for example, through water flow. The release of radionuclides to the environment can also be delayed by the construction of engineered barrier systems between the waste and the geologic formation. Corrosion engineers are participating in the design and the performance prediction of the engineered barriers. The principal engineered component in this multibarrier approach is the container for the waste. Beyond the metallic containers, other engineered barriers could be …

The shape dependence of the catalytic reduction of NO by CO on Rhodium nanopolyhedra and nanocubes was studied from 230-270 C. The nanocubes are found to exhibit higher turnover frequency and lower activation energy than the nanopolyhedra. These trends are compared to previous studies on Rh single crystals.