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Analytic ICF Hohlraum Energetics (open access)

Analytic ICF Hohlraum Energetics

We apply recent analytic solutions to the radiation diffusion equation to problems of interest for ICF hohlraums. The solutions provide quantitative values for absorbed energy which are of use for generating a desired radiation temperature vs. time within the hohlraum. Comparison of supersonic and subsonic solutions (heat front velocity faster or slower, respectively, than the speed of sound in the x-ray heated material) suggests that there may be some advantage in using high Z metallic foams as hohlraum wall material to reduce hydrodynamic losses, and hence, net absorbed energy by the walls. Analytic and numerical calculations suggest that the loss per unit area might be reduced {approx} 20% through use of foam hohlraum walls. Reduced hydrodynamic motion of the wall material may also reduce symmetry swings, as found for heavy ion targets.
Date: August 27, 2003
Creator: Rosen, M D & Hammer, J
System: The UNT Digital Library
Thermo-Gas-Dynamic Model of Afterburning in Explosions (open access)

Thermo-Gas-Dynamic Model of Afterburning in Explosions

A theoretical model of afterburning in explosions created by turbulent mixing of the detonation products from fuel-rich charges with air is described. It contains three key elements: (i) a thermodynamic-equilibrium description of the fluids (fuel, air, and products), (ii) a multi-component gas-dynamic treatment of the flow field, and (iii) a sub-grid model of molecular processes of mixing, combustion and equilibration.
Date: July 27, 2003
Creator: Kuhl, A L; Ferguson, R E & Bell, J B
System: The UNT Digital Library
The National Ignition Facility: the World's Largest Optics and Laser System (open access)

The National Ignition Facility: the World's Largest Optics and Laser System

The National Ignition Facility, a center for the study of high energy density plasma physics and fusion energy ignition, is currently under construction at the Lawrence Livermore National Laboratory. The heart of the NIF is a frequency tripled, flashlamp-pumped Nd:glass laser system comprised of 192 independent laser beams. The laser system is capable of generating output energies of 1.8MJ at 351nm and at peak powers of 500 TW in a flexible temporal pulse format. A description of the NIF laser system and its major components is presented. We also discuss the manufacture of nearly 7500 precision large optics required by the NIF including data on the manufactured optical quality vs. specification. In addition, we present results from an on-going program to improve the operational lifetime of optics exposed to high fluence in the 351-nm section of the laser.
Date: January 27, 2003
Creator: Moses, E I; Campbell, J H; Stolz, C J & Wuest, C R
System: The UNT Digital Library
zPicture: Dynamic Alignment and Visualization Tool for Analyzing Conservation Profiles (open access)

zPicture: Dynamic Alignment and Visualization Tool for Analyzing Conservation Profiles

None
Date: October 27, 2003
Creator: Ovcharenko, I; Loots, G G; Hardison, R C; Miller, W; Stubbs, L; Paar, H et al.
System: The UNT Digital Library
Hydrodynamic Issues in PAMS Mandrel Target Fabrication (open access)

Hydrodynamic Issues in PAMS Mandrel Target Fabrication

Imperfections in PAMS mandrels critically govern the quality of final ICF targets. Imperfections in the mandrels can have a wide range of origins. Here, they present observations of 3 types of imperfections, and data to support the proposal that hydrodynamic factors during the curing of the mandrel are potential causes of these imperfections.
Date: August 27, 2003
Creator: McQuillan, B M; Paguio, R; Subramanian, P; Takagi, M & Zebib, A
System: The UNT Digital Library
Materials Science under Extreme Conditions of Pressure and Strain Rate (open access)

Materials Science under Extreme Conditions of Pressure and Strain Rate

Solid state dynamics experiments at very high pressures (P >> 10 GPa) and strain rates ({var_epsilon} >> 10{sup 5} s{sup -1}) have been demonstrated on high energy laser facilities, albeit over brief intervals of time and small spatial scales. We have developed two methods for driving samples to high pressures (10-100 GPa) at high strain rate (10{sup 6}-10{sup 8} s{sup -1}) in the solid state. One method uses a shockless compression technique, and the other uses multiple staged shocks. These drives are calibrated with VISAR measurements of the resulting compression wave. Deformation mechanisms are inferred under these conditions by characterizing recovered samples. Material strength at high pressures and strain rates is deduced by measuring the reduced growth of material perturbations at a hydrodynamically unstable interface. Microscopic lattice response is determined by time-resolved Bragg diffraction and x-ray absorption spectroscopy (EXAFS). Large-scale simulations, both at the continuum level using constitutive models and at the lattice level using molecular dynamics simulation, are used to interpret these integral experiments. We will review our progress in this new area of laser-based materials science research, then present a vision for carrying these solid-state experiments to much higher pressures, P > 1000 GPa, on the National Ignition …
Date: March 27, 2003
Creator: Remington, B. A.; Bazan, G.; Bringa, E.; Caturla, M.; Edwards, M. J.; Glendinning, S. G. et al.
System: The UNT Digital Library
The Next Generation Microlensing Search: SuperMacho (open access)

The Next Generation Microlensing Search: SuperMacho

Past microlensing experiments such as the MACHO project have discovered the presence of a larger than expected number of microlensing events toward the Large Magellanic Cloud (LMC). These events could represent a large fraction of the dark matter in the halo of our Galaxy, if they are indeed due to halo lenses. However the locations of most of the lenses are poorly defined. The SuperMacho project will detect and follow up {approx}60 microlensing events exhibiting special properties due to binarity, etc., will allow us to better determine the location and nature of the lenses causing the LMC microlensing events.
Date: October 27, 2003
Creator: Drake, A.; Cook, K.; Hiriart, R.; Keller, S.; Miknaitis, G.; Nilolaev, S. et al.
System: The UNT Digital Library
Modeling of Thermal Convection of Liquid TNT for Cookoff (open access)

Modeling of Thermal Convection of Liquid TNT for Cookoff

The objective is to computationally model thermal convection of liquid TNT in a heated cylindrical container for what are called 'cookoff' experiments. Our goal is to capture the thermal convection coupled to the heat transfer in the surrounding container. We will present computational results that validate the functionality of the model, numerical strategy, and computer code for a model problem with Rayleigh number of O(10{sup 6}). We solve the problem of thermal convection between two parallel plates in this turbulent flow regime and show that the three-dimensional computations are in excellent agreement with experiment.
Date: February 27, 2003
Creator: McCallen, R; Dunn, T; Nichols, A; Reaugh, J & McClelland, M
System: The UNT Digital Library
International Cooperation to Address the Radioactive Legacy in States of the Former Soviet Union (open access)

International Cooperation to Address the Radioactive Legacy in States of the Former Soviet Union

The end of the Cold War allows a comprehensive assessment of the nature and extent of the residual contamination derivative from the atomic defense and nuclear power enterprise in the former Soviet Union. The size of the problem is considerable; some 6.3 x 10{sup 7} TBq (6.4 x 10{sup 8} m{sup 3}) of radioactive waste from the Soviet Union weapons and power complex was produced throughout all stages of the nuclear fuel cycle. The resulting contamination occurs at sites throughout the former Soviet Union where nuclear fuels were mined, milled, enriched, fabricated, and used in defense and power reactors. In addition, liquid radioactive wastes from nuclear reprocessing have been discharged to lakes, rivers, reservoirs and other surface impoundments; military and civilian naval reactor effluents were released to sea as well as stabilized on land. Finally, nuclear testing residuals from atmospheric and underground nuclear tests at the Semipalatinsk and Novaya Zemlya test sites and peaceful nuclear tests conducted throughout the area of the former Soviet Union pose risks to human health and the environment. Through a program of international scientific exchange, cooperative approaches to address these threats provide former Soviet scientists with expertise and technologies developed in the United States, Europe, …
Date: July 27, 2003
Creator: Smith, D. K.; Knapp, R. B.; Rosenberg, N. D. & Tompson, A. F. B.
System: The UNT Digital Library
A Multiresolution Image Cache for Volume Rendering (open access)

A Multiresolution Image Cache for Volume Rendering

The authors discuss the techniques and implementation details of the shared-memory image caching system for volume visualization and iso-surface rendering. One of the goals of the system is to decouple image generation from image display. This is done by maintaining a set of impostors for interactive display while the production of the impostor imagery is performed by a set of parallel, background processes. The system introduces a caching basis that is free of the gap/overlap artifacts of earlier caching techniques. instead of placing impostors at fixed, pre-defined positions in world space, the technique is to adaptively place impostors relative to the camera viewpoint. The positions translate with the camera but stay aligned to the data; i.e., the positions translate, but do not rotate, with the camera. The viewing transformation is factored into a translation transformation and a rotation transformation. The impostor imagery is generated using just the translation transformation and visible impostors are displayed using just the rotation transformation. Displayed image quality is improved by increasing the number of impostors and the frequency that impostors are re-rendering is improved by decreasing the number of impostors.
Date: February 27, 2003
Creator: LaMar, E. & Pascucci, V.
System: The UNT Digital Library
Dislocation-Defect Interactions in Materials (open access)

Dislocation-Defect Interactions in Materials

In order to develop predictive models of the mechanical response of irradiated materials it is necessary to understand the fundamental physical processes controlling the deformation. This is particularly important near yielding where local defect interactions may dominate the behavior. Dislocation-defect interactions in copper containing various densities and distributions of stacking-fault tetrahedra and small dislocation loops were examined dynamically using the in-situ TEM straining technique. Defect annihilation mechanisms as well as the conditions required to produce defect-free channels are proposed. The experimental results are compared to atomistic simulations and continuum mechanics calculations of unit interactions. Based on these observations, an improved continuum model of the mechanical behavior of irradiated materials is presented.
Date: February 27, 2003
Creator: Robach, J. S.; Robertson, I. M.; Ahn, D. C.; Sofronis, P.; Wirth, B. D. & Arsenlis, T.
System: The UNT Digital Library
Update from the''Radiochemistry at RIA'' Symposium held March 27, 2003 at the 225th American Chemical Society Meeting in New Orleans, LA (open access)

Update from the''Radiochemistry at RIA'' Symposium held March 27, 2003 at the 225th American Chemical Society Meeting in New Orleans, LA

The scientific case for a new high-luminosity Radioactive Ion Beam (RIB) Facility capable of accelerating essentially the entire chart of the nuclides has been established over more than a decade.[1-5] Preliminary experiments at new facilities [6] are promising as the frontier of RIB research continues to expand at a fast rate. Radiochemical experiments that could benefit many areas of research, from the basic science of astrophysics and heavy element research, to the applied science of Stockpile Stewardship and medical isotope production, are now for the first time being seriously discussed for a RIB facility. This paper attempts to highlight some areas of research which may be significantly enhanced should a premier radiochemical facility be co-located at a RIB accelerator facility such as the Rare Isotope Accelerator (RIA). The purpose of the symposium was to gather together experts in a variety of fields to discuss the harvesting of radionuclides and its potential benefits to research in the heavy element research, medical isotope production, Stockpile Stewardship Program (SSP), environmental research, medical astrophysics and educational fields. Preliminary ideas as to the engineering features required for such a facility at RIA, to generate, handle, store and use isotopes of interest potentially containing very high …
Date: May 27, 2003
Creator: Stoyer, M A & Mantica, P F
System: The UNT Digital Library
CFD Modeling For Urban Air Quality Studies (open access)

CFD Modeling For Urban Air Quality Studies

The computational fluid dynamics (CFD) approach has been increasingly applied to many atmospheric applications, including flow over buildings and complex terrain, and dispersion of hazardous releases. However there has been much less activity on the coupling of CFD with atmospheric chemistry. Most of the atmospheric chemistry applications have been focused on the modeling of chemistry on larger spatial scales, such as global or urban airshed scale. However, the increased attentions to terrorism threats have stimulated the need of much more detailed simulations involving chemical releases within urban areas. This motivated us to develop a new CFD/coupled-chemistry capability as part of our modeling effort.
Date: October 27, 2003
Creator: Lee, R L; Lucas, L J; Humphreys, T D & Chan, S T
System: The UNT Digital Library
DETERMINING THE RATIO OF THE H+ YIELDS TV TO H+ YIELDS TB DECAY RATES FOR LARGE TAN BETA AT THE LARGE HADRON COLLIDER. (open access)

DETERMINING THE RATIO OF THE H+ YIELDS TV TO H+ YIELDS TB DECAY RATES FOR LARGE TAN BETA AT THE LARGE HADRON COLLIDER.

We present results on the determination of the observable ratio R = BR(H{sup +} {yields} {tau}{sup +}{nu}{sup -})/BR(H{sup +} {yields} t{bar b}) of charged Higgs boson decay rates as a discriminant quantity between Supersymmetric and non-Supersymmetric models. Simulation of measurements of this quantity through the analysis of the charged Higgs production process gb {yields} tbH{sup +} and relative backgrounds in the two above decay channels has been performed in the context of ATLAS. A {approx} 12-14% accuracy on R can be achieved for tan {beta} = 50, m{sub H{sup {+-}}} = 300-500 GeV and after an integrated luminosity of 300 fb{sup -1}. With this precision measurement, the Large Hadron Collider (LHC) can easily discriminate between models for the two above scenarios, so long as tan {beta} > 20.
Date: May 27, 2003
Creator: ASSAMAGAN,K. A. GUASCH,J. MORETTI,S. PENARANDA,S.
System: The UNT Digital Library
Advanced Transmission Electron Microscopy of Pu Alloys (open access)

Advanced Transmission Electron Microscopy of Pu Alloys

The characterization of microstructural changes in Pu-Ga alloys resulting from storage and aging phenomena is an important technical challenge to the nuclear Stockpile Stewardship program. We have identified at least two age-related phenomena that may occur in Pu alloys, dimensional changes due to the initial transient, helium accumulation, and void swelling, and phase instability. The initial transient is a well-known effect that results from the initial cascade damage. This form of dimensional change tends to saturate within approximately two years. A second contributor to dimensional change is the build-up of helium as a result of the alpha decay. Helium is generated at a rate of approximately 40 parts per million per year. Positron annihilation results by Howell indicate that the helium atoms will quickly fill a nearby vacancy and diffuse through the lattice as a helium filled vacancy. Void swelling is potentially the most severe mechanism of dimensional change in Pu alloys. It has been observed in all-materials exposed to irradiation, but has yet to be seen in naturally aged Pu. Phase instability is a potential concern due to the fact that the {delta}-phase is thermodynamically metastable at room temperature. Timofeeva has shown that the {delta}-phase will decompose to {delta}-phase …
Date: January 27, 2003
Creator: Schwartz, A J; Wall, M A; Wolfer, W G & Moore, K T
System: The UNT Digital Library
Electron Beam/Converter Target Interactions in Radiographic Accelerators (open access)

Electron Beam/Converter Target Interactions in Radiographic Accelerators

Linear induction accelerators used in X-ray radiography have single-pulse parameters of the order 20 MeV of electron beam energy, 2 kA of beam current, pulse lengths of 50-100 ns, and spot sizes of 1-2 mm. The thermal energy deposited in a bremsstrahlung converter target made of tantalum from such a pulse is {approx}80 kJ/cc, more than enough to bring the target material to a partially ionized state. The tail end of a single beam pulse, or any subsequent pulse in a multi-pulse train, undergoes a number of interactions with the target that can affect beam transport and radiographic performance. Positive ions extracted from the target plasma by the electron beam space charge can affect the beam focus and centroid stability. As the target expands on the inter-pulse time scale, the integrated line density of material decreases, eventually affecting the X-ray output of the system. If the target plume becomes sufficiently large, beam transport through it is affected by macroscopic charge and current neutralization effects and microscopic beam/plasma instability mechanisms. We will present a survey of some of these interactions, as well as some results of an extensive experimental and theoretical campaign to understand the practical amelioration of these effects, carried …
Date: May 27, 2003
Creator: McCarrick, J.; Caporaso, G.; Chambers, F.; Chen, Y. J.; Falabella, S.; Goldin, F. et al.
System: The UNT Digital Library
A Coal-Fired Power Plant with Zero Atmospheric Emissions (open access)

A Coal-Fired Power Plant with Zero Atmospheric Emissions

This paper presents the thermodynamic analysis of a coal-based zero-atmospheric emissions electric power plant. The approach involves an oxygen-blown coal gasification unit. The resulting synthetic gas (syngas) is combusted with oxygen in a gas generator to produce the working fluid for the turbines. The combustion produces a gas mixture composed almost entirely of steam and carbon dioxide. These gases drive multiple turbines to produce electricity. The turbine discharge gases pass to a condenser where water is captured. A stream of carbon dioxide then results that can be used for enhanced oil recovery, or for sequestration. This analysis is based on a 400 MW electric power generating plant that uses turbines that are currently under development by a U.S. turbine manufacturer. The power plant has a net thermal efficiency of 42.6%. This efficiency is based on the lower heating value of the coal, and includes the energy necessary for coal gasification, air separation and for carbon dioxide separation and sequestration. The paper also presents an analysis of the cost of electricity (COE) and the cost of conditioning carbon dioxide for sequestration for the 400 MW power plant. Electricity cost is compared for three different gasification processes (Texaco, Shell, and Koppers-Totzek) and …
Date: May 27, 2003
Creator: Martinez-Frias, J; Aceves, S M; Smith, J R & Brandt, H
System: The UNT Digital Library
Prospects for high-gain, high yield NIF targets driven by 2w (green) light (open access)

Prospects for high-gain, high yield NIF targets driven by 2w (green) light

A laser ablation/ionization mass spectrometer system is described for the direct analysis of solids, particles, and fibers. The system uses a quadrupole ion trap operated in an ion-storage (IS) mode, coupled with a reflectron time-of-flight mass spectrometer (TOF-MS). The sample is inserted radially into the ring electrode and an imaging system allows direct viewing and selected analysis of the sample. Measurements identified trace contaminants of Ag, Sn, and Sb in a Pb target with single laser-shot experiments. Resolution (m/{Delta}m) of 1500 and detection limits of approximately 10 pg have been achieved with a single laser pulse. The system configuration and related operating principles for accurately measuring low concentrations of isotopes are described.
Date: October 27, 2003
Creator: Suter, L J; Glenzer, S; Haan, S; Hammel, B; Manes, K; Meezan, N et al.
System: The UNT Digital Library
Crossed Beam Energy Transfer in the NIF ICF Target Design (open access)

Crossed Beam Energy Transfer in the NIF ICF Target Design

In the National Ignition Facility (NIF) ICF point design, the cylindrical hohlraum target is illuminated by multiple laser beams through two laser entrance holes on the ends. According to simulations by LASNEX and HYDRA plasma created inside the hohlraum will stream out of the LEH, accelerate to supersonic speeds and then fan out radially. Inside the hohlraum, flows are subsonic. Forward Brillouin scattering can transfer energy between pairs of laser beams (0 and 1) if the following frequency matching condition is satisfied: {omega}{sub 0} - {omega}{sub 1} = (k{sub 0} - k{sub 1}) {center_dot} V + |k{sub 0} - k{sub 1}| c{sub s} (1) where {omega}{sub 0.1} and k{sub 0.1} are the frequencies and wave-numbers of the two laser beams, V is the plasma flow velocity and c{sub s} is the local ion sound speed. In the nominal case of equal frequency beams, this requires the component of the plasma flow velocity transverse to the bisector of the beam directions to be sonic, with the resulting transfer being to the downstream beam. In the NIF beam geometry, this is from the outer to inner cones of beams. The physics of this transfer is the same as in beam bending; the …
Date: August 27, 2003
Creator: Williams, E. A.; Hinkel, D. E. & Hittinger, J. A.
System: The UNT Digital Library
Experiments and Simulations of Penetration into Granite by an Aluminum Shaped Charge (open access)

Experiments and Simulations of Penetration into Granite by an Aluminum Shaped Charge

This paper describes experimental results and numerical simulations of jet penetration into granite from an aluminum lined shaped charge. Several penetration versus standoff experiments were conducted into an in-situ granite formation located in the Climax Ridge region of the Nevada Test Site. Simulations of the jet penetration were modeled with a two dimensional arbitrary lagrange eulerian hydrocode. The effects of variations in the granite flow stress, porosity, and EOS have been evaluated. The work described in this paper is a continuation of our studies on jet penetration and modeling into high strength concrete.
Date: July 27, 2003
Creator: Murphy, M. J.; Randers-Pehrson, G.; Kuklo, R. M.; Rambur, T. A.; Switzer, L. L. & Summes, M. A.
System: The UNT Digital Library
Using Graphs for Fast Error Term Approximation of Time-varying Datasets (open access)

Using Graphs for Fast Error Term Approximation of Time-varying Datasets

We present a method for the efficient computation and storage of approximations of error tables used for error estimation of a region between different time steps in time-varying datasets. The error between two time steps is defined as the distance between the data of these time steps. Error tables are used to look up the error between different time steps of a time-varying dataset, especially when run time error computation is expensive. However, even the generation of error tables itself can be expensive. For n time steps, the exact error look-up table (which stores the error values for all pairs of time steps in a matrix) has a memory complexity and pre-processing time complexity of O(n2), and O(1) for error retrieval. Our approximate error look-up table approach uses trees, where the leaf nodes represent original time steps, and interior nodes contain an average (or best-representative) of the children nodes. The error computed on an edge of a tree describes the distance between the two nodes on that edge. Evaluating the error between two different time steps requires traversing a path between the two leaf nodes, and accumulating the errors on the traversed edges. For n time steps, this scheme has …
Date: February 27, 2003
Creator: Nuber, C; LaMar, E C; Pascucci, V; Hamann, B & Joy, K I
System: The UNT Digital Library
Positron Annihilation Spectroscopy and Small Angle Neutron Scattering Characterization of the Effect of Mn on the Nanostructural Features formed in Irradiated Fe-Cu-Mn Alloys (open access)

Positron Annihilation Spectroscopy and Small Angle Neutron Scattering Characterization of the Effect of Mn on the Nanostructural Features formed in Irradiated Fe-Cu-Mn Alloys

The size, number density and composition of the nanometer defects responsible for the hardening and embrittlement in irradiated Fe-0.9wt.% Cu and Fe-0.9wt.% Cu-1.0wt% Mn model reactor pressure vessel alloys were measured using small angle neutron scattering and positron annihilation spectroscopy. These alloys were irradiated at 290 C to relatively low neutron fluences (E > 1 MeV, 6.0 x 10{sup 20} to 4.0 x 10{sup 21} n/m{sup 2}) in order to study the effect of manganese on the nucleation and growth of copper rich precipitates and secondary defect features. Copper rich precipitates were present in both alloys following irradiation. The Fe-Cu-Mn alloy had smaller precipitates and a larger number density of precipitates, suggesting Mn segregation at the iron matrix-precipitate interface which reduces the interfacial energy and in turn the driving force for coarsening. Mn also retards the precipitation kinetics and inhibits large vacancy cluster formation, suggesting a strong Mn-vacancy interaction which reduces radiation enhanced diffusion.
Date: February 27, 2003
Creator: Glade, S C; Wirth, B D; Asoka-Kumar, P; Odette, G R; Sterne, P A & Howell, R H
System: The UNT Digital Library
The Madden-Julian Oscillation in General Circulation Models (open access)

The Madden-Julian Oscillation in General Circulation Models

A methodology is utilized to analyze in a standardized fashion the Madden-Julian Oscillation (MJO) in general circulation models. This is attained by projecting 20-100 day bandpass filtered outgoing longwave radiation (OLR) from the models onto the two leading empirical orthogonal functions (EOF's) of observed OLR that characterize the propagation of MJO convection from the Indian Ocean to the central Pacific Ocean. The resulting principal component time series are then screened to isolate boreal winters during which they exhibit a lead-lag relationship consistent with observations. This PC subset is used for linear regression to determine the ability of the models to simulate the observed spacetime variability of the MJO. The vast majority of models underestimate the amplitude of the MJO convective anomalies by a factor of two or more, and the eastward propagation of convection is less coherent than observed, typically. For a given family of models, coupling to an ocean leads to better organization of the large-scale convection. The low-level moisture convergence mechanism for eastward propagation is represented in limited cases, as is the vertical structure of the MJO.
Date: October 27, 2003
Creator: Sperber, K R; Gleckler, P J; Doutriaux, C; Groups, A M; Groups, C M; Slingo, J M et al.
System: The UNT Digital Library
The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potnt sources of replication errors in vivo; Henderson, P.T.; Delaney, J.C.; Muller, J. G.; Neeley, W.L.; Tannenbaum, S.R.; Burrows, C.J.; Essigmann, J.M. Biochemistry (accelerate (open access)

The hydantoin lesions formed from oxidation of 7,8-dihydro-8-oxoguanine are potnt sources of replication errors in vivo; Henderson, P.T.; Delaney, J.C.; Muller, J. G.; Neeley, W.L.; Tannenbaum, S.R.; Burrows, C.J.; Essigmann, J.M. Biochemistry (accelerate

Single-stranded DNA genomes have been constructed that site-specifically contain the 8-oxo-7,8-dihydro-2'-deoxyguanine (8-oxoG) oxidation products guanidinohyndantoin (Gh), and the two stable stereoisomers of spiroiminodihydantoin (Sp1 and Sp2). The circular viral genomes were transfected into wild-type AB1157 Escherichia coli and the efficiency of lesion bypass by DNA polymerase(s) was assessed. Viral progeny were analyzed for mutation frequency and type using the recently developed restriction endonuclease and post-labeling (REAP) assay. Gh was bypassed nearly as efficiently as the parent 8-oxoG, but was highly mutagenic, causing almost exclusive G{yields}C transversions. The stereoisomers Sp1 and Sp2 were, in comparison, much stronger blocks to DNA polymerase extension, and caused a mixture of G{yields}T and G{yields}C transversions. The ratio of G{yields}T to G{yields}C mutations for each Sp lesion was dependent on the stereochemical configuration of the base. All observed mutation frequencies were at least an order of magnitude higher than those caused by 8-oxoG. Were these lesions to be formed in vivo, our data show that they are absolutely miscoding, and may be refractory to repair after translesion synthesis.
Date: October 27, 2003
Creator: Henderson, P. T.; Delaney, J. C.; Muller, J. G.; Neeley, W. L.; Tannenbaum, S. R.; Burrows, C. J. et al.
System: The UNT Digital Library