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Use of a transfected and amplified Drosophila heat shock promoter construction for inducible production of toxic mouse c-myc proteins in CHO cells
After transfection and selection with methotrexate, CHO cell lines were established which contained up to 2000 copies of an expression vector for c-myc protein. The vector contained the Drosophila heat shock protein 70 (hsp70) promoter fused with the coding region of the mouse c-myc gene. Incubation of cells for up to 3 hours at 43/sup 0/C resulted in at least a 100-fold induction of recombinant c-myc mRNA. When cells were shifted back to 37/sup 0/C, within 1 to 4 hours, this RNA was translated into protein to yield about 250 ..mu..g per 10/sup 9/ cells. Cells died a few hours later, suggesting that high concentrations of intracellular c-myc are cytotoxic. 47 refs., 5 figs.
Date:
July 24, 1987
Creator:
Wurm, F.M.; Gwinn, K.A.; Papoulas, O.; Pallavicini, M. & Kingston, R.E.
System:
The UNT Digital Library
1987 Gordon Research Conference on Physical Metallurgy.
None
Date:
July 24, 1987
Creator:
unknown
System:
The UNT Digital Library