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Produce for victory
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Beat the promise
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U.S. Army official poster.
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Investigating the role of CheA-3 in Dusulfovibrio Vulgaris Hildenborough
Multiple sets of chemotaxis genes including three cheA homologs were identified in the genome sequence of the anaerobic bacterium Desulfovibrio vulgaris Hildenborough. Each CheA is a histidine kinase (HK) and part of a two component signal transduction system. Knock out mutants in the three cheA genes were created using single cross-over homologous recombination insertion. We studied the phenotypes of the cheA mutants in detail and discovered that ?cheA-3 has a non swarming/swimming phenotype both in the soft agar plates and Palleroni chamber assays. CheA-3 shows similarity to the Shewanella oneidensis CheA-3 and the Vibrio cholerae CheA-2 that are responsible for chemotaxis in the respective organisms. We did not find any morphological or structural differences between the three Delta cheA mutants and the wild type cells in electron microscopy. Our results from these studies are presented.
Date:
May 22, 2010
Creator:
Ray, Jayashee; Keller, Kimberley; Krierim, Bernhard; Auer, Manfred; Keasling, Jay; Wall, Judy et al.
System:
The UNT Digital Library
Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough
Desulfovibrio vulgaris is an anaerobic sulfate-reducing bacterium capable of facilitating the removal of toxic metals such as uranium from contaminated sites via reduction. As such, it is essential to understand the intricate regulatory cascades involved in how D. vulgaris and its relatives respond to stressors in such sites. One approach is the identification and analysis of small non-coding RNAs (sRNAs); molecules ranging in size from 20-200 nucleotides that predominantly affect gene regulation by binding to complementary mRNA in an anti-sense fashion and therefore provide an immediate regulatory response. To identify sRNAs in D. vulgaris, a bacterium that does not possess an annotated hfq gene, RNA was pooled from stationary and exponential phases, nitrate exposure, and biofilm conditions. The subsequent RNA was size fractionated, modified, and converted to cDNA for high throughput transcriptomic deep sequencing. A computational approach to identify sRNAs via the alignment of seven separate Desulfovibrio genomes was also performed. From the deep sequencing analysis, 2,296 reads between 20 and 250 nt were identified with expression above genome background. Analysis of those reads limited the number of candidates to ~;;87 intergenic, while ~;;140 appeared to be antisense to annotated open reading frames (ORFs). Further BLAST analysis of the intergenic …
Date:
May 17, 2010
Creator:
Burns, Andrew; Joachimiak, Marcin; Deutschbauer, Adam; Arkin, Adam & Bender, Kelly
System:
The UNT Digital Library
Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris
D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.
Date:
May 17, 2010
Creator:
Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin & Mukhopadhyay, Aindrila
System:
The UNT Digital Library
Syntrophic Degradation of Lactate in Methanogenic Co-cultures
In environments where the amount of the inorganic electron acceptors (oxygen, nitrate, sulfate, sulfur oroxidized metal ions (Fe3+;Mn4+) is insufficient for complete breakdown of organic matter, methane is formed as the major reduced end product. In such methanogenic environments organic acids are degraded by syntrophic associations of fermenting, acetogenic bacteria (e.g., sulfate-reducing bacteria (SRB) as"secondary fermenters") and methanogenic archaea. In these consortia, the conversion of lactate to acetate, CO2 and methane depends on the cooperating activities of both metabolically distinct microbial groups that are tightly linked by the need to maintain the exchanged metabolites (hydrogenandformate) at very low concentrations.
Date:
May 17, 2010
Creator:
Meyer, Birte & Stahl, David
System:
The UNT Digital Library
Two Component Signal Transduction in Desulfovibrio Species
The environmentally relevant Desulfovibrio species are sulfate-reducing bacteria that are of interest in the bioremediation of heavy metal contaminated water. Among these, the genome of D. vulgaris Hildenborough encodes a large number of two component systems consisting of 72 putative response regulators (RR) and 64 putative histidinekinases (HK), the majority of which are uncharacterized. We classified the D. vulgaris Hildenborough RRs based on their output domains and compared the distribution of RRs in other sequenced Desulfovibrio species. We have successfully purified most RRs and several HKs as His-tagged proteins. We performed phospho-transfer experiments to verify relationships between cognate pairs of HK and RR, and we have also mapped a few non-cognate HK-RR pairs. Presented here are our discoveries from the Desulfovibrio RR categorization and results from the in vitro studies using purified His tagged D. vulgaris HKs and RRs.
Date:
May 17, 2010
Creator:
Luning, Eric; Rajeev, Lara; Ray, Jayashree & Mukhopadhyay, Aindrila
System:
The UNT Digital Library
Phylogenetic Analysis of Shewanella Strains by DNA Relatedness Derived from Whole Genome Microarray DNA-DNA Hybridization and Comparison with Other Methods
Phylogenetic analyses were done for the Shewanella strains isolated from Baltic Sea (38 strains), US DOE Hanford Uranium bioremediation site [Hanford Reach of the Columbia River (HRCR), 11 strains], Pacific Ocean and Hawaiian sediments (8 strains), and strains from other resources (16 strains) with three out group strains, Rhodopseudomonas palustris, Clostridium cellulolyticum, and Thermoanaerobacter ethanolicus X514, using DNA relatedness derived from WCGA-based DNA-DNA hybridizations, sequence similarities of 16S rRNA gene and gyrB gene, and sequence similarities of 6 loci of Shewanella genome selected from a shared gene list of the Shewanella strains with whole genome sequenced based on the average nucleotide identity of them (ANI). The phylogenetic trees based on 16S rRNA and gyrB gene sequences, and DNA relatedness derived from WCGA hybridizations of the tested Shewanella strains share exactly the same sub-clusters with very few exceptions, in which the strains were basically grouped by species. However, the phylogenetic analysis based on DNA relatedness derived from WCGA hybridizations dramatically increased the differentiation resolution at species and strains level within Shewanella genus. When the tree based on DNA relatedness derived from WCGA hybridizations was compared to the tree based on the combined sequences of the selected functional genes (6 loci), we …
Date:
May 17, 2010
Creator:
Wu, Liyou; Yi, T. Y.; Van Nostrand, Joy & Zhou, Jizhong
System:
The UNT Digital Library
Microarray-based analysis of survival of soil microbial community during ozonation
A 15 h ozonation was performed on bioremediated soil to remove recalcitrant residual oil. To monitor the survival of indigenous microorganisms in the soil during in-situ chemical oxidation(ISCO) culturing and a functional genearray, GeoChip, was used to examine the functional genes and structure of the microbial community during ozonation (0h, 2h, 4h, 6h, 10hand15h). Breakthrough ozonation decreased the population of cultivable heterotrophic bacteria by about 3 orders of magnitude. The total functional gene abundance and diversity decreased during ozonation, as the number of functional genes was reduced by 48percent after 15 h. However, functional genes were evenly distributed during ozonation as judged by the Shannon-Weaver Evenness index. A sharp decrease in gene number was observed in the first 6 h of ozonation followed by a slower decrease in the next 9 h, which was consistent with microbial populations measured by a culture based method. Functional genes involved in carbon, nitrogen, phosphors and sulfur cycling, metal resistance and organic remediation were detected in all samples. Though the pattern of gene categories detected was similar for all time points, hierarchica lcluster of all functional genes and major functional categories all showed a time-serial pattern. Bacteria, archaea and fungi decreased by 96.1percent, 95.1percent …
Date:
May 17, 2010
Creator:
Wang, Jian; Van Nostrand, Joy D.; He, Zhili; Wu, Liyou; Deng, Ye; Zhang, Xu et al.
System:
The UNT Digital Library
Hispanic Women in the Labor Force
Infographic showing statistics pertaining to Hispanic women employment in 2015. The pamphlet compares wages and conditions of Hispanic women with women of other races.
Date:
unknown
Creator:
United States. Women's Bureau.
System:
The UNT Digital Library
Working Mothers in the U.S.
Infographic of working mothers in the United States based upon data from 2012 American Community Survey, U.S. Census Bureau (ACS), Pew Research Center Analysis of Decennial Census (2011), and the Bureau of Labor Statistics, Current Population Survey.
Date:
2013
Creator:
[United States. Department of Labor.]
System:
The UNT Digital Library
50 Years Later: Women, Work & the Work Ahead
Infographic commemorating the 50th anniversary of the American Women Report by outlining statistics and major events related to women's education and participation in the workforce from 1963 to 2012.
Date:
2012
Creator:
United States. Women's Bureau.
System:
The UNT Digital Library
Equal Pay
Infographic providing information on the inequity of pay between women and men in the United States job market.
Date:
unknown
Creator:
United States. Women's Bureau.
System:
The UNT Digital Library
Effectiveness of Family Planning Methods
Single page chart with graphical information on a variety of contraception methods and their percentage of effectiveness.
Date:
2011~
Creator:
United States. Department of Health and Human Services, Centers for Disease Control and Prevention
System:
The UNT Digital Library
Synchrotron-based high-pressure research in materials science
None
Date:
unknown
Creator:
unknown
System:
The UNT Digital Library
Kinetic Controls on the Desorption/Dissolution of Sorbed U(VI) and Their Influence on Reactive Transport
None
Date:
April 19, 2007
Creator:
Zachara, J. M.; Liu, C.; Qafoku, N.; McKinley, J. P.; Davis, J. A.; Stoliker, D. et al.
System:
The UNT Digital Library
Development of Modeling Methods and Tools for Predicting Coupled Reactive Transport Processes in Porous Media at Multiple Scales
None
Date:
April 19, 2007
Creator:
Kanel, S. R.; Loganathan, V. A.; Jeppu, G.; Kumar, A.; Srinivasan, V.; Radu, T. et al.
System:
The UNT Digital Library
Mechanistically-Based Field-Scale Models of Uranium Biogeochemistry from Upscaling Pore-Scale Experiments and Models
Effective environmental management of DOE sites requires reliable prediction of reactive transport phenomena. A central issue in prediction of subsurface reactive transport is the impact of multiscale physical, chemical, and biological heterogeneity. Heterogeneity manifests itself through incomplete mixing of reactants at scales below those at which concentrations are explicitly defined (i.e., the numerical grid scale). This results in a mismatch between simulated reaction processes (formulated in terms of average concentrations) and actual processes (controlled by local concentrations). At the field scale, this results in apparent scale-dependence of model parameters and inability to utilize laboratory parameters in field models. Accordingly, most field modeling efforts are restricted to empirical estimation of model parameters by fitting to field observations, which renders extrapolation of model predictions beyond fitted conditions unreliable. The objective of this project is to develop a theoretical and computational framework for (1) connecting models of coupled reactive transport from pore-scale processes to field-scale bioremediation through a hierarchy of models that maintain crucial information from the smaller scales at the larger scales; and (2) quantifying the uncertainty that is introduced by both the upscaling process and uncertainty in physical parameters. One of the challenges of addressing scale-dependent effects of coupled processes in …
Date:
April 19, 2007
Creator:
Scheibe, Tim; Tartakovsky, Alexandre; Wood, Brian & Seymour, Joe
System:
The UNT Digital Library
Impacts of Climate Variability on the California Current Ecosystem and Pacific Salmon Survival: Linkages, Ocean Condition Indicators, Forecasting, and Management Perspectives
None
Date:
May 19, 2008
Creator:
Casillas, Edmundo & Peterson, William T.
System:
The UNT Digital Library
Applying EMSL Capabilities to Biogeochemistry and Environmental Research
The Environmental Molecular Sciences laboratory (EMSL) is a national scientific user facility operated by the Pacific Northwest National Laboratory (PNNL) for the U.S. Department of Energy's Office of Biological and Environmental Research. Located in Richland, Washington, EMSL offers researchers a comprehensive array of cutting-edge capabilities unmatched anywhere else in the world and access to the expertise of over 300 resident users--all at one location. EMSL's resources are available on a peer-reviewed proposal basis and are offered at no cost if research results are shared in the open literature. Researchers are encouraged to submit a proposal centered around one of EMSL's four Science Themes, which represent growing areas of research: (1) Geochemistry/Biogeochemistry and Subsurface Science; (2) Atmospheric Aerosol Chemistry; (3) Biological Interactions and Dynamics; and (4) Science of Interfacial Phenomena. To learn more about EMSL, visit www.emsl.pnl.gov.
Date:
April 19, 2007
Creator:
Felmy, Andy
System:
The UNT Digital Library
Photoconductive Decay Lifetime and Suns-Voc Diagnostics of Efficient Heterojunction Solar Cells
None
Date:
May 1, 2008
Creator:
Page, M. R.; Iwaniczko, E.; Xu, Y.; Roybal, L.; Bauer, R.; Yuan, H.-C. et al.
System:
The UNT Digital Library
Synthesis of Goethite-Coated Sand and Analysis of its Interactions with Uranium
None
Date:
April 19, 2007
Creator:
Loganathan, Vijay A.; Kanel, Sushil R.; Barnett, Mark O. & Clement, and T. Prabhakar
System:
The UNT Digital Library
Comparison of Predictive Models for PV Module Performance (Presentation)
This paper examines three models used to estimate the maximum power (P{sub m}) of PV modules when the irradiance and PV cell temperature are known: (1) the power temperature coefficient model, (2) the PVFORM model, and (3) the bilinear interpolation model. A variation of the power temperature coefficient model is also presented that improved model accuracy. For modeling values of P{sub m}, an 'effective' plane-of-array (POA) irradiance (E{sub e}) and the PV cell temperature (T) are used as model inputs. Using E{sub e} essentially removes the effects of variations in solar spectrum and reflectance losses, and permits the influence of irradiance and temperature on model performance for P{sub m} to be more easily studied. Eq. 1 is used to determine E{sub e} from T and the PV module's measured short-circuit current (I{sub sc}). Zero subscripts denote performance at Standard Reporting Conditions (SRC).
Date:
May 1, 2008
Creator:
Marion, B.
System:
The UNT Digital Library
Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution
This poster describes the Changes in Microbial Community Structure During Biostimulation for Uranium Reduction at Different Levels of Resolution
Date:
April 19, 2007
Creator:
Hwang, C.; Wu, W.-M.; Gentry, T.J.; Corbin, G.; Carley, J.; Carroll, S.L. et al.
System:
The UNT Digital Library
Coupled Processes Influencing the Transport of Uranium over Multiple Scales
None
Date:
April 19, 2007
Creator:
Mayes, Melanie A.; Tang, Guoping; Parker, Jack C.; Perfect, Ed & van den Berg, Elmer
System:
The UNT Digital Library
Influence of Wetting and Mass Transfer Properties of Organic Chemical Mixtures in Vadose Zone Materials on Groundwater Contamination by Nonaqueous Phase Liquids
None
Date:
April 19, 2007
Creator:
Werth, Charles; Valocchi, Albert; Yoon, Hongkyu; Nellis, Scott; Prescod, Garvin & Oostrom, Mart
System:
The UNT Digital Library