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Environmental Whole-Genome Amplification to Access Microbial Diversity in Contaminated Sediments
Low-biomass samples from nitrate and heavy metal contaminated soils yield DNA amounts that have limited use for direct, native analysis and screening. Multiple displacement amplification (MDA) using ?29 DNA polymerase was used to amplify whole genomes from environmental, contaminated, subsurface sediments. By first amplifying the genomic DNA (gDNA), biodiversity analysis and gDNA library construction of microbes found in contaminated soils were made possible. The MDA method was validated by analyzing amplified genome coverage from approximately five Escherichia coli cells, resulting in 99.2 percent genome coverage. The method was further validated by confirming overall representative species coverage and also an amplification bias when amplifying from a mix of eight known bacterial strains. We extracted DNA from samples with extremely low cell densities from a U.S. Department of Energy contaminated site. After amplification, small subunit rRNA analysis revealed relatively even distribution of species across several major phyla. Clone libraries were constructed from the amplified gDNA, and a small subset of clones was used for shotgun sequencing. BLAST analysis of the library clone sequences showed that 64.9 percent of the sequences had significant similarities to known proteins, and ''clusters of orthologous groups'' (COG) analysis revealed that more than half of the sequences from …
Date:
December 10, 2005
Creator:
Abulencia, C. B.; Wyborski, D. L.; Garcia, J.; Podar, M.; Chen, W.; Chang, S. H. et al.
Object Type:
Article
System:
The UNT Digital Library
[Christmas and Kwanzaa Volunteer Soiree]
Video recording from The Black Academy of Arts and Letters recorded during their Christmas and Kwanzaa Volunteer Soiree event in 2005. This video features a celebration to shows appreciation to TBAAL volunteer, members, and sponsors in friendship and entertainment in the spirit of Christmas and Kwanzaa.
Date:
December 10, 2005
Creator:
Boyd, Kenneth
Object Type:
Video
System:
The UNT Digital Library
The Baytown Sun (Baytown, Tex.), Vol. 85, No. 1, Ed. 1 Saturday, December 10, 2005
Daily newspaper from Baytown, Texas that includes local, state, and national news along with advertising.
Date:
December 10, 2005
Creator:
Cash, Wanda Garner
Object Type:
Newspaper
System:
The Portal to Texas History
Perry Daily Journal (Perry, Okla.), Vol. 112, No. 236, Ed. 1 Saturday, December 10, 2005
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
December 10, 2005
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History