Fluorescence properties of purified isoleucyl-tRNA-synthetase isolated from E. coli B have been studied. No changes in the quantum yield, energy or polarization of the emission were detected in the presence (either individually or in combinations) of the substrates and cofactors required for activation of L-isoleucine. In 2.5 M urea enzyme activity and intrinsic fluorescence intensity (at 340 nm) each decrease with time, showing similar kinetics and rate constants. The rate of this decay is reduced in the presence of ligands which can bind to the enzyme and the effect has been used to measure dissociation constants for enzyme-ligand complexes. Values have been obtained for the complexes between enzyme and L-isoleucine (K{sub diss} = 2.5 x 10{sup -5} M), L-valine (K{sub diss} = 3.0 x 10{sup -4} M), ATP (K{sub diss} = 1.5 x 10{sup -4} M) and PP{sub i} (K{sub diss} = 2.0 x 10{sup -4} M) at 25{sup o}. The effects of ionic strength, and the temperature dependence and urea concentration dependence of L-isoleucine binding have also been studied. Magnesium ions, which are required for catalysis, do not greatly affect the binding of single substrates, but changes are seen in the presence of ATP and L-isoleucine together. The magnesium …

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The purpose of this analysis is to determine if the two piece flat head closure concept presently being considered by INC for the LOFT reactor vessel will meet the structural and fatigue requirements of Article 4 of Section III, ''Nuclear Vessels,'' of the ASME Boiler and Pressure Vessel Code, for the anticipated LOFT operational requirements. The analyses performed include stress analyses based on classical stress analysis methods and proven computer programs, fatigue analyses based on the fatigue criteria of Section III of the Code, and thermal analyses based on multi-dimensional computer programs.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether acceptance by a city councilman of the position of executive director of a community action agency violates Article 373, Vernon's Penal Code, or Article 988, Vernon's Civil Statutes.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Authority of Texas Board of Examiners in the Fitting and Dispensing pf Hearing Aids to conduct educational seminars to licensed and temporary trainees in the hearing aid profession.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Does a waste water treatment plant qualify under the laws of this state as a "project" eligible for loans and partial or complete participation of ownership by the state from the Water Development Fund?

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Authority of county clerk to issue a marriage license to persons presently legally married to each other, and related question.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether a County Commissioner has vacated his office by moving from the precinct from which he was elected to office to another part of the county.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Must the Adjutant General of Texas Comply with Fed. Ex. Order No. 1426.1, although to do so apparently would conflict with Art. 5154c, V.C.S.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Election of a Chairman of the Texas Tourist Development Board.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Taxability of bank shares of stock for ad valorem tax purposes under Articles 7165 or 7166, Vernon's Civil Statutes, where the bank is not doing business.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Contract Between Texas Water Quality Board and Gulf Coast Waste Disposal Authority

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Refund of monies paid under Article 666-32 1/2 (b) Vernon's Penal Code, for calling of local option election.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Grant of Airport Aid Funds to the City of Texarkana, Texas under Article 46c-6, Subdivision 10, V. C. S.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: May the Commissioners' Court of Harrison County provide funds for the defense of the Sheriff of its County, and his Deputies, in a civil rights action against them in U. S.District Court, South Dakota, under the stated facts.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether a corporation doing business as a detective agency must file a surety bond under both Articles 1302-3-3.04 and 4413 (29bb), Vernon;s Civil Statutes.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether Price Daniel is eligible for appointment to the Texas Supreme Court in view of the fact his son is a member of the House of Representatives?

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Whether the Texas Department of Agriculture may legally enter into a certain contract for payment of a monthly consultation fee.

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: Is the Texas Barber Law violated when the hair of a male customer is out or trimmed while fitting him for a wig by a person without a license under that law?

Document issued by the Office of the Attorney General of Texas in Austin, Texas, providing an interpretation of Texas law. It provides the opinion of the Texas Attorney General, Crawford Martin, regarding a legal question submitted for clarification: The proper method of determining the payment for accumulated vacation and sick leave to be paid the estates of deceased State employees.