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ISOLEUCYL-tRNA-SYNTHETASE A FLUORESCENCE STUDY OP THE BINDINGPROPERTIES OF THE SYNTHETASE FROM ESCHERICHIA COLI
Fluorescence properties of purified isoleucyl-tRNA-synthetase isolated from E. coli B have been studied. No changes in the quantum yield, energy or polarization of the emission were detected in the presence (either individually or in combinations) of the substrates and cofactors required for activation of L-isoleucine. In 2.5 M urea enzyme activity and intrinsic fluorescence intensity (at 340 nm) each decrease with time, showing similar kinetics and rate constants. The rate of this decay is reduced in the presence of ligands which can bind to the enzyme and the effect has been used to measure dissociation constants for enzyme-ligand complexes. Values have been obtained for the complexes between enzyme and L-isoleucine (K{sub diss} = 2.5 x 10{sup -5} M), L-valine (K{sub diss} = 3.0 x 10{sup -4} M), ATP (K{sub diss} = 1.5 x 10{sup -4} M) and PP{sub i} (K{sub diss} = 2.0 x 10{sup -4} M) at 25{sup o}. The effects of ionic strength, and the temperature dependence and urea concentration dependence of L-isoleucine binding have also been studied. Magnesium ions, which are required for catalysis, do not greatly affect the binding of single substrates, but changes are seen in the presence of ATP and L-isoleucine together. The magnesium …
Date:
November 1, 1970
Creator:
Penzer, Geoffrey R.; Bennett, Edward L. & Calvin, Melvin.
System:
The UNT Digital Library
National program plan for work to establish fission-product behavior in HTGR systems
None
Date:
November 10, 1970
Creator:
Bell, W. E.
System:
The UNT Digital Library
Preliminary analysis of LOFT Reactor vessel closure. Addendum I, Volume I. Appendix A-3
The purpose of this analysis is to determine if the two piece flat head closure concept presently being considered by INC for the LOFT reactor vessel will meet the structural and fatigue requirements of Article 4 of Section III, ''Nuclear Vessels,'' of the ASME Boiler and Pressure Vessel Code, for the anticipated LOFT operational requirements. The analyses performed include stress analyses based on classical stress analysis methods and proven computer programs, fatigue analyses based on the fatigue criteria of Section III of the Code, and thermal analyses based on multi-dimensional computer programs.
Date:
November 1, 1970
Creator:
unknown
System:
The UNT Digital Library
Production Reactor Branch monthly reports, January--October 1970
This document provides the monthly reports of the Production Reactor Branch for the months of January 1970 through October 1970.
Date:
November 16, 1970
Creator:
Plum, R. L.
System:
The UNT Digital Library
Technical bases for K Reactor dichromate and pH control
This memorandum was written to provide additional technical basis for preparing Process Standards. Fuel surface-tube outlet temperature data is also provided. These data will help establish dichromate concentration and coolant pH based on the outlet coolant temperatures of the hottest reactor process tubes.
Date:
November 12, 1970
Creator:
Larrick, A. P.
System:
The UNT Digital Library
Pacific Northwest Laboratory monthly report to the Division of Isotopes Development for October 1970
None
Date:
November 15, 1970
Creator:
Hansen, J. E.
System:
The UNT Digital Library
M001-STR04-W234ald summary report CY 1970. For period ending 30 September 1970
None
Date:
November 1, 1970
Creator:
unknown
System:
The UNT Digital Library
Development of particles coated with pyrolytic carbon. IX. Studies of commercial experimental coated particles
None
Date:
November 1, 1970
Creator:
Baxman, H. R.; Bertino, J. P.; Bard, R. J.; Hayter, S. W.; O'Rourke, J. A. & Levinson, L. S.
System:
The UNT Digital Library
Trip to Los Alamos, New Mexico
None
Date:
November 1, 1970
Creator:
Salvador, L.A.
System:
The UNT Digital Library
Nuclear Physics Laboratory Progress Report, November 1, 1969--October 31, 1970.
None
Date:
November 1970
Creator:
unknown
System:
The UNT Digital Library
Gas Coverage for Pigma Welding.
None
Date:
November 10, 1970
Creator:
Brandon, E. D. & Bush, W. L.
System:
The UNT Digital Library
Optimization of Reactor Thermal Design by Statistical Hot Spot Analysis.
None
Date:
November 1, 1970
Creator:
Amendola, A.
System:
The UNT Digital Library
Materials properties data book. Addendum
None
Date:
November 15, 1970
Creator:
unknown
System:
The UNT Digital Library
Use of Hastelloy X-280 in reactor core test alternate conceptual design
None
Date:
November 24, 1970
Creator:
Swenson, L.D.
System:
The UNT Digital Library
Conceptual Core Model for the Reactor Core Test
This report addresses conceptual core model for the reactor core test.
Date:
November 16, 1970
Creator:
Swenson, L.D.
System:
The UNT Digital Library
Trip report to Warren, Ohio (meeting: coordination group for Military Handbook 5)
None
Date:
November 17, 1970
Creator:
Layland, E. L.
System:
The UNT Digital Library
Trip to Westinghouse Industrial Equipment Division, Sykesville, Maryland
None
Date:
November 1, 1970
Creator:
Pahler, R. A.
System:
The UNT Digital Library
Reactor Outage Schedule (Tentative) [November 1970]
None
Date:
November 1, 1970
Creator:
Rowe, R.P.
System:
The UNT Digital Library
E.S. table look-up bug
None
Date:
November 1, 1970
Creator:
White, R. H.
System:
The UNT Digital Library
Reactor outage schedule (tentative)
None
Date:
November 15, 1970
Creator:
Rowe, R.P.
System:
The UNT Digital Library