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Comparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action (open access)

Comparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action

This article uses a discovery driven comparative proteomics analysis to reveal unique early signaling response of the yeast Saccharomyces cerevisiae on the proteome level to oxidants with a different mechanism of action as early as 3 min after treatment with four oxidants, namely H₂O₂, cumene hydroperoxide (CHP), and menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap).
Date: December 26, 2020
Creator: Pandey, Prajita; Zaman, Khadiza; Prókai, László, 1958- & Shulaev, Vladimir
Object Type: Article
System: The UNT Digital Library
Angiogenesis in the Avian Embryo Chorioallantoic Membrane: A Perspective on Research Trends and a Case Study on Toxicant Vascular Effects (open access)

Angiogenesis in the Avian Embryo Chorioallantoic Membrane: A Perspective on Research Trends and a Case Study on Toxicant Vascular Effects

This article considers areas of cardiovascular research that have benefited from studies of the chorioallantoic membrane (CAM), including the themes of investigation of the CAM’s hemodynamic influence on heart and central vessel development, use of the CAM as a model vascular bed for studying angiogenesis, and the CAM as an assay tool.
Date: October 28, 2020
Creator: Burggren, Warren W. & Antich, Maria Rojas
Object Type: Article
System: The UNT Digital Library
The Tetracentron genome provides insight into the early evolution of eudicots and the formation of vessel elements (open access)

The Tetracentron genome provides insight into the early evolution of eudicots and the formation of vessel elements

Article reporting a chromosome-scale assembly of the T. sinense genome.
Date: December 2, 2020
Creator: Dixon, R. A.; Liu, Ping-Li; Zhang, Xi; Mao, Jian-Feng; Hong, Yan-Ming; Zhang, Ren-Gang et al.
Object Type: Article
System: The UNT Digital Library
Draft Genome of the Common Snapping Turtle, Chelydra serpentina, a Model for Phenotypic Plasticity in Reptiles (open access)

Draft Genome of the Common Snapping Turtle, Chelydra serpentina, a Model for Phenotypic Plasticity in Reptiles

Article studying climate adaptation using common snapping turtles, Chelydra serpentina, as the model species. The genome and genetic variants identified here provide a foundation for future studies of adaptation to climate.
Date: December 1, 2020
Creator: Crossley, Dane A., II; Das, Debojyoti; Singh, Sunil Kumar; Bierstedt, Jacob; Erickson, Alyssa; Galli, Gina L. J. et al.
Object Type: Article
System: The UNT Digital Library
Editorial: Biofuels and Bioenergy (open access)

Editorial: Biofuels and Bioenergy

Editorial on the research topic of biofuels and bioenergy.
Date: November 30, 2020
Creator: Barros-Rios, Jaime; Zhang, Jin & Lu, Mengzhu
Object Type: Article
System: The UNT Digital Library
How Tiny Zebrafish and Their Surprisingly Human-Like Genome Can Benefit Medical Research captions transcript

How Tiny Zebrafish and Their Surprisingly Human-Like Genome Can Benefit Medical Research

Presentation highlighting the use of zebrafish to identify genes relating to predisposition to thromboembolism and cardiovascular disease in people with African ancestry. It was presented at the UNT Transdisciplinary Conference on Ancestral Genomics Research which was held virtually on November 20-21, 2020.
Date: November 21, 2020
Creator: Jagadeeswaran, Pudur
Object Type: Video
System: The UNT Digital Library
RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish (open access)

RNaseH-mediated simultaneous piggyback knockdown of multiple genes in adult zebrafish

This article tests if it is possible to piggyback more than one antisense deoxyoligonucleotide (dO) with one vivo morpholino (VMO). The authors previously developed a piggyback knockdown method that was used to knockdown genes in adult zebrafish. In this article, the authors develop a method to knockdown three genes at one time, and by increasing the concentration of VMO by twofold, could knockdown six genes simultaneously. These multiple gene knockdowns will not only increase the efficiency of the method in whole genome-wide knockdowns but will also be useful to study multifactorial disorders.
Date: November 19, 2020
Creator: Raman, Revathi; Ryon, Mia & Jagadeeswaran, Pudur
Object Type: Article
System: The UNT Digital Library
CaMV35S promoter – A plant biology and biotechnology workhorse in the era of synthetic biology (open access)

CaMV35S promoter – A plant biology and biotechnology workhorse in the era of synthetic biology

This review article calls for establishing the CaMV 35S promoter as a quantitative reference standard for transcription activity in plants.
Date: November 18, 2020
Creator: Amack, Stephanie C. & Antunes, Mauricio S.
Object Type: Article
System: The UNT Digital Library
Were domestic camelids present on the prehispanic South American agricultural frontier? An ancient DNA study (open access)

Were domestic camelids present on the prehispanic South American agricultural frontier? An ancient DNA study

Article testing the hypothesis that domesticated camelids occurred in prehispanic, southern Mendoza through analysis of ancient DNA.
Date: November 5, 2020
Creator: Johnson, Jeff A.; Kim, Tracy; Wolverton, Steven J.; Gil, Adolfo Fabian; Abbona, Cinthia Carolina & Adolfo, Gustavo Neme
Object Type: Article
System: The UNT Digital Library
Effect of increased embryonic temperature during developmental windows on survival, morphology and oxygen consumption of rainbow trout (Oncorhynchus mykiss) (open access)

Effect of increased embryonic temperature during developmental windows on survival, morphology and oxygen consumption of rainbow trout (Oncorhynchus mykiss)

This article applies a developmental 3-dimensional critical window experimental design for a vertebrate, using time, temperature, and phenotypic response (i.e., variables measured). To assess thermal effects on fish development, the authors examined trout hatchling phenotypes following rearing in constant temperatures (5, 10, 15 and 17.5 °C) and following exposure to increased temperature above 5 °C during specific developmental windows.
Date: November 2, 2020
Creator: Melendez, Christopher L. & Mueller, Casey A.
Object Type: Article
System: The UNT Digital Library
Maternal serum concentration of anti-Müllerian hormone is a better predictor than basal follicle stimulating hormone of successful blastocysts development during IVF treatment (open access)

Maternal serum concentration of anti-Müllerian hormone is a better predictor than basal follicle stimulating hormone of successful blastocysts development during IVF treatment

Article is a study that aims to identify actionable clinical and culture characteristics of IVF treatment that influence blastocyst developmental rate, with the goal of acquiring optimal success.
Date: October 12, 2020
Creator: Burggren, Warren W.; Herrington, Richard; Sadruddin, Sheela; Barnett, Brian; Ku, Lowell; Havemann, Dara et al.
Object Type: Article
System: The UNT Digital Library
Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana (open access)

Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana

This article finds that the transcript profile of the laccase gene ChLAC8 parallels the accumulation of C-lignin during seed coat development. Recombinant ChLAC8 oxidizes caffeyl and sinapyl alcohols, generating their corresponding dimers or trimers in vitro, but cannot oxidize coniferyl alcohol. The authors propose a basis for this substrate preference based on molecular modeling/docking experiments. Suppression of ChLAC8 expression led to significantly reduced C-lignin content in the seed coats of transgenic Cleome plants. Feeding of 13C-caffeyl alcohol to the Arabidopsis (Arabidopsis thaliana) caffeic acid o-methyltransferase mutant resulted in no incorporation of 13C into C-lignin, but expressing ChLAC8 in this genetic background led to appearance of C-lignin with >40% label incorporation. These results indicate that ChLAC8 is required for C-lignin polymerization and determines lignin composition when caffeyl alcohol is available.
Date: October 9, 2020
Creator: Wang, Xin; Zhuo, Chunliu; Xiao, Xirong; Wang, Xiaoqiang; Docampo-Palacios, Maite; Chen, Fang et al.
Object Type: Article
System: The UNT Digital Library
Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish (open access)

Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish

Article aims to identify other proteases that may activate FVII using zebrafish as a model.
Date: September 22, 2020
Creator: Khandekar, Gauri; Iyer, Neha & Jagadeeswaran, Pudur
Object Type: Article
System: The UNT Digital Library
Nest-site selection and breeding success of passerines in the world’s southernmost forests (open access)

Nest-site selection and breeding success of passerines in the world’s southernmost forests

This article studies the nest-site selection and survival rate of the five most abundant open-cup forest-dwelling passerines (Elaenia albiceps, Zonotrichia capensis, Phrygilus patagonicus, Turdus falcklandii, and Anairetes parulus) in the world’s southernmost forests of Navarino Island, in the Cape Horn Biosphere reserve, Chile (55°S).
Date: February 5, 2020
Creator: Jara Miller, Rocio F.; Crego, Ramiro D.; Samuel, Michael D.; Rozzi, Ricardo, 1960- & Jiménez, Jaime E.
Object Type: Article
System: The UNT Digital Library
Metallic nanoparticle production and exposure/deposition system for toxicological research applications using zebrafish (open access)

Metallic nanoparticle production and exposure/deposition system for toxicological research applications using zebrafish

Article presents a method that provides a novel way to synthesize NPs and expose biological organisms for various toxicological analysis.
Date: September 3, 2020
Creator: Nayek, Subhayu; Aguilar, Roberto; Juel, Lauren A. & Verbeck, Guido F.
Object Type: Article
System: The UNT Digital Library
Comparative Diet Analysis of Barn Owl (Tyto alba) and Long- Eared Owl (Asio otus) in a Habitat Island in North-Central Texas (open access)

Comparative Diet Analysis of Barn Owl (Tyto alba) and Long- Eared Owl (Asio otus) in a Habitat Island in North-Central Texas

Article filling the regional gap in knowledge of diet composition of the barn owl (Tyto alba) and the long-eared owl (Asio otus). The study was conducted at the Lewisville Lake Environmental Learning Area (LLELA) and examined the remains found in owl pellets collected September 2016 to March 2017.
Date: August 22, 2020
Creator: Jiménez, Jaime E.; Jones, Matthew K.; Stoddart, Kaitlyn; Dickson, Sentell; Chapman, Abigail; Baxter-Slye, Jaime L. et al.
Object Type: Article
System: The UNT Digital Library
Workflow for the Quantification of Soluble and Insoluble Carbohydrates in Soybean Seed (open access)

Workflow for the Quantification of Soluble and Insoluble Carbohydrates in Soybean Seed

This article develops and validates a sequential protocol for quantifying reserve and structural carbohydrates in soybean seed. It was successfully applied to eight soybean genotypes harvested from two locations, and the resulting correlations of carbohydrate and oil or protein are presented. This methodology has the potential not only to guide soybean cultivar optimization processes but also to be expanded to other crops with only slight modifications.
Date: July 30, 2020
Creator: Moretti, Ademar; Arias, Cintia L.; Mozzoni, Leandro A.; Chen, Pengyin; McNeece, Brant T.; Mian, M. A. Rouf et al.
Object Type: Article
System: The UNT Digital Library
Parental transgenerational epigenetic inheritance related to dietary crude oil exposure in Danio rerio (open access)

Parental transgenerational epigenetic inheritance related to dietary crude oil exposure in Danio rerio

Article investigating the transgenerational inheritance from both parental lines in zebrafish. Adult zebrafish were separated into female and male groups exposed for 21 days to either a control diet or to a diet containing water accommodated fractions of crude oil. To determine the maternal and paternal influence on their offspring, the authors evaluated responses from molecular to whole organismal levels in both generations.
Date: January 22, 2020
Creator: Bautista, Naim M.; Crespel, Amélie; Crossley, Janna; Padilla, Pamela A. & Burggren, Warren W.
Object Type: Article
System: The UNT Digital Library
Proanthocyanidin Biosynthesis—a Matter of Protection (open access)

Proanthocyanidin Biosynthesis—a Matter of Protection

Article discussing key questions and opportunities in the field of Proanthocyanidins (PA) biosynthesis.
Date: August 18, 2020
Creator: Dixon, R. A. & Sarnala, Sai
Object Type: Article
System: The UNT Digital Library
Exploring the socio-economic and environmental components of infectious diseases using multivariate geovisualization: West Nile Virus (open access)

Exploring the socio-economic and environmental components of infectious diseases using multivariate geovisualization: West Nile Virus

Article focusing on West Nile Virus (WNV), a mosquito borne pathogen, as a case study for spatial data visualization of environmental characteristics of a vector’s habitat alongside human demographic composition for understanding potential public health risks of infectious disease.
Date: March 2, 2020
Creator: Kala, Abhishek K.; Atkinson, Samuel F. & Tiwari, Chetan
Object Type: Article
System: The UNT Digital Library
MYB30 Orchestrates Systemic Reactive Oxygen Signaling and Plant Acclimation (open access)

MYB30 Orchestrates Systemic Reactive Oxygen Signaling and Plant Acclimation

This article explores the relationship among MYB30, the ROS wave, and systemic acclimation in Arabidopsis by determining the SAA response to HL stress of MYB30 mutants and wild-type plants.
Date: July 22, 2020
Creator: Fichman, Yosef; Zandalinas, Sara I.; Sengupta, Soham; Burks, David J.; Meyers, Ronald J., Jr.; Azad, Rajeev K. et al.
Object Type: Article
System: The UNT Digital Library
SEIPIN Isoforms Interact with the Membrane-Tethering Protein VAP27-1 for Lipid Droplet Formation (open access)

SEIPIN Isoforms Interact with the Membrane-Tethering Protein VAP27-1 for Lipid Droplet Formation

Article demonstrating that part of the function of two SEIPIN isoforms in Arabidopsis (Arabidopsis thaliana), AtSEIPIN2 and AtSEIPIN3, may depend on their interaction with the vesicle-associated membrane protein (VAMP)–associated protein (VAP) family member AtVAP27-1. The authors used a combination of biochemical, cell biology, and genetics approaches to show that AtVAP27-1 interacts with the N termini of AtSEIPIN2 and AtSEIPIN3 and likely supports the normal formation of LDs. This insight indicates that the ER membrane tethering machinery in plant cells could play a role with select SEIPIN isoforms in LD biogenesis at the ER, and additional experimental evidence in Saccharomyces cerevisiae supports the possibility that this interaction may be important in other eukaryotic systems.
Date: July 20, 2020
Creator: Greer, Michael Scott; Cai, Yingqi; Gidda, Satinder K.; Esnay, Nicolas; Kretzschmar, Franziska K.; Seay, Damien C. et al.
Object Type: Article
System: The UNT Digital Library
A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity (open access)

A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity

Article is a study with the goal of optimizing and validating the use of a colorimetric plate-based respiratory burst and fluorometric plate-based phagocytic cell activity assays for use with kidney cells from the fathead minnow (Pimephales promelas), an emerging immunotoxicity model.
Date: July 18, 2020
Creator: Thornton Hampton, Leah M.; Venables, Barney J. & Jeffries, Marlo K. Sellin
Object Type: Article
System: The UNT Digital Library
Lipophilic signals lead to organ-specific gene expression changes in Arabidopsis seedlings (open access)

Lipophilic signals lead to organ-specific gene expression changes in Arabidopsis seedlings

Article presents a study where whole transcriptome sequencing (RNA-seq) and differential expression analysis were used to identify early (1–3 hr) transcriptional changes induced by the exogenous treatment of NAE 18:2 and NAE 18:3 in cotyledons, roots, and seedlings.
Date: July 15, 2020
Creator: Cannon, Ashley E.; Yan, Chengshi; Burks, David J.; Rao, Xiaolan; Azad, Rajeev K. & Chapman, Kent Dean
Object Type: Article
System: The UNT Digital Library