The title compound, C₂₆H₂₁Cl₂NO, crystallizes with two symmetry-independent molecules in the asymmetric unit.

The activity of plant 9-lipoxygenases (LOXs) influences the outcome of Arabidopsis thaliana interaction with pathogen and insects. Evidence provided here indicates that in Arabidopsis, 9-LOXs facilitate infestation by Myzus persicae, commonly known as the green peach aphid (GPA), a sap-sucking insect, and infection by the fungal pathogen Fusarium graminearum. in comparison to the wild-type plant, lox5 mutants, which are deficient in a 9-lipoxygenase, GPA population was smaller and the insect spent less time feeding from sieve elements and xylem, thus resulting in reduced water content and fecundity of GPA. LOX5 expression is induced rapidly in roots of GPA-infested plants. This increase in LOX5 expression is paralleled by an increase in LOX5-synthesized oxylipins in the root and petiole exudates of GPA-infested plants. Micrografting experiments demonstrated that GPA population size was smaller on plants in which the roots were of the lox5 mutant genotype. Exogenous treatment of lox5 mutant roots with 9-hydroxyoctadecanoic acid restored water content and population size of GPA on lox5 mutants. Together, these results suggest that LOX5 genotype in roots is critical for facilitating insect infestation of Arabidopsis. in Arabidopsis, 9-LOX function is also required for facilitating infection by F. graminearum, which is a leading cause of Fusarium head …

The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summer semester at the University of North Texas. This class is a team taught effort by Professors O'Donovan and Kunz. The course is constructed as an intensive practice exercise to teach the student about gene mutations, biosynthetic pathways, preparation and analysis of plasmid DNA, and many other topics included in the notebook.

This article evaluates HCT down-regulated alfalfa plants for their forage composition and agronomic performance in the greenhouse and under field conditions.

Article describes study in which mRNA expression of iGluR was quantified in two sensory neuron clusters of two cohorts of Aplysia californica (Aplysia) at both sexual maturity (~8 months) and advanced age (~12 months) in order to examine if changes in ionotropic L-Glu receptor (iGluR) transcription underlie reduced physiology8

Article on an anthraquinone derivative, emodin sensitizes hepatocellular carcinoma cells to TRAIL induced apoptosis through the induction of death receptors and downregulation of cell survival proteins.

This article examines the use of metal-containing polymers as a novel class of antivirals. Polymers containing platinum and to a lesser extent tin, have repeatedly demonstrated antitumor activity in vitro and in vivo against a variety of cell and tumor types. The mechanisms responsible for the antitumor activity include inducing a delay in cell proliferation and sister chromatid exchanges blocking tumor growth.

This review explores the latest developments in Fourier transform mass spectrometry and Orbitrap based metabolomics technology, its advantages and drawbacks for using in metabolomics and lipidomics studies, and development of novel approaches for processing high resolution mass spectrometry data.

Article on bacterial communities of the coronal sulcus and distal urethra of adolescent males.

Four species of net-spinning caddisflies, Hydropsyche simulans Ross, Cheumatopsyche lasia Ross, Cheumatopsyche campyla Ross and Chimarra obscura (Walker) are common in the regulated portions of the Brazos River. Hydropsyche simulans spun capture nets with the largest meshdimensions; the two Cheumatopsyche species' nets had the next largest meshes, and Chimarra obscura spun nets with the smallest dimensions. Cheumatopsyche lasia and C. campyla constructed nets with similar sized meshes. The number of individuals m~2 and standing crop biomass were not significantly different among low, medium, and high velocities. Early hydropsychid instars fed on detritus while later instar H. simulans and C. campyla had larger proportions of animal material. Guts of later instar C. lasia individuals had a greater percentage of algae and diatoms.

Article describes study which investigated the contributions of both genetic and plastic components for differences in body shape in two species of Lake Malawi cichlids using wild‐caught specimens and a common garden experiment.

This article focuses on recent advances in brassinosteroid-regulated cell wall biosynthesis and remodeling in response to stresses, comparing the authors' understanding of the mechanisms in grass species with those in the more studied dicots.

Article describing the breeding strategies (i.e., nest dimensions, nest height from the ground, egg laying rhythm, clutch size, length of the developmental periods, breeding phenology, and diversity of nesting substrate) of five passerine birds that inhabit sub-Antarctic ecosystems.

Article on the characterization of an isoflavonoid-specific prenyltransferase from Lupinus albus.

This article tests if lignin content can be further reduced by combining genetic mutations in C1 metabolism and the lignin biosynthetic pathway by generating and functionally characterizing fpgs1ccoaomt1 double mutants. The observations demonstrate that additional reduction in lignin content and improved sugar release can be achieved by simultaneous downregulation of a gene in the C1 (FPGS1) and lignin biosynthetic (CCOAOMT) pathways.

Article describes study in which fpgs1ccoaomt1 double mutants were generated and functionally characterized in order to test if lignin content can be further reduced by combining genetic mutations in C1 metabolism and the lignin biosynthetic pathway.

A comparative study was made of environmental variables and the density, biomass, diversity, and species composition of macroinvertebrates in areas downstream from a dam with a hypolimnetic release (Hungry Horse Dam on the Flathead River) and a dam with a selective withdrawal system (Libby Dam on the Kootenai River). A major objective of this study was to examine the response of macroinvertebrate communities to defined environmental gradients in temperature, flow, substrate, and food-related variables (periphyton, particulate organic carbon in the seston). In addition, the effects of experimental manipulations in discharge on macroinvertebrate drift and stranding were assessed, and the effects of temperature on the growth rates and emergence of five species of insects were measured.

Article presents research on oxalate catabolism in plants.

This article presents a study in which an in silico genome-scale metabolic model of steroidogenesis is used to investigate the effects of hypoxic stress on steroid hormone productions in fish. This article introduces a powerful model for pathway analysis that can lend insights on the effects of various stressor scenarios on metabolic functions.

Article on the detection and quantification of engineered proanthocyanidins in transgenic plants.

This article posits that the frequently abrupt environmental changes accompanying much longer-term general climate change require consideration of the true nature of environmental change coupled with an expansion of focus to consider developmental phenotypic plasticity.

Article on the draft genome sequence of Cupriavidus sp. strain SK-4, a di-ortho-substituted biphenyl-utilizing bacterium isolated from polychlorinated biphenyl-contaminated sludge.

This article presents research on genetic and biochemical analyses that shows ANR has dual activity and is involved not only in the production of (−)-epicatechin starter units but also in the formation of 2,3-cis-leucocyanidin to serve as (−)-epicatechin extension units. The results find that differences in the product specificities of ANRs account for the presence/absence of PA polymerization and the compositions of PAs across plant species

This article reports that a major monomer unit of the lignin polymer can function as a signal molecule to trigger proteolysis of the enzyme L-phenylalanine ammonia-lyase, the entry point into the lignin biosynthetic pathway, and feedback regulate the expression levels of lignin biosynthetic genes.