The mechanisms by which hyperthermophilic archaebacteria grow and carry out metabolic functions at elevated temperatures have yet to be determined. The objective of this work is to develop an understanding of the metabolic characteristics of, and the electron transport enzymes involved in, hydrogen/sulfur transformation by hyperthermophilic archaebacteria. Efforts focus on the autotrophic H{sub 2}-oxidizing bacterium, Pyrodictium brockii which has an optimum growth temperature of 105{degrees}C. Biochemical and genetic characterization of enzymes involved in hydrogen oxidizing electron transport pathway. These including investigating the role of the membrane lipids in protecting the hydrogenase enzyme from thermal inactivation, characterization of a quinone and a c-type cytochrome, and analysis of the topology in the membrane in the net energy generating components are reported. The long-term goal is to understand some of the factors contributing to the biochemical basis of extreme thermophily.

The mechanisms by which hyperthermophilic archaebacteria grow and carry out metabolic functions at elevated temperatures have yet to be determined. The objective of this work is to develop an understanding of the metabolic characteristics of, and the electron transport enzymes involved in, hydrogen/sulfur transformation by hyperthermophilic archaebacteria. Efforts focus on the autotrophic H{sub 2}-oxidizing bacterium, Pyrodictium brockii which has an optimum growth temperature of 105{degrees}C. Biochemical and genetic characterization of enzymes involved in hydrogen oxidizing electron transport pathway. These including investigating the role of the membrane lipids in protecting the hydrogenase enzyme from thermal inactivation, characterization of a quinone and a c-type cytochrome, and analysis of the topology in the membrane in the net energy generating components are reported. The long-term goal is to understand some of the factors contributing to the biochemical basis of extreme thermophily.

K-capture to positron branching ratios were measured in the decay of Na/ sup 22/, Co/sup 58/, and Ga/sup 68/ -- all pure Gamow-Teller emitters, using coincidence scintillation spectrometer techniques. The measured values are 0.105 plus or minus 0.004 for Na/sup 22/, 5.08 plus or minus 0.17 for Co/sup 58/, and 1.28 plus or minus 0.12 and 0.10 plus or minus 0.02 for Ga/sup 58/. From these the Fierz interference terra is computed to be b =--0.004 plus or minus 0.012, -0.004 plus or minus 0.14, --0-03 plus or minus 0.02, and +0.03 plus or minus 0.01, respectively. These results indicate that the Fierz interference in Gamow-Teller interaction is very small. The decay of 270 day Ge/sup 68/ was investigated in equilibrium with Ga/sup 68/ to look for a possible low-lying 0+ level in Ga/sup 68/ using x-ray--x-ray and x-ray- gamma -ray coincidences. The result was negative. Besides the 9 kev K x ray, the 1.07 Mev gamma ray in the decay of Ga/sup 68/ and annihilation radiation, no other gamma rays were detected (<8% of 1.07 Mev gamma ray). The number of positrons per 1.07 Mev gamma - quantum was determined as 19.47 plus or minus 2.10. The ratio of …

This report describes results on six years of research on how bacteria store nickel and then mobilize it into the active center of enzymes.

This report deals with the development of methods and techniques for measuring the amounts of radioisotope on the stack wall, in ash, stack gas and atmosphere during and after incineration in the conventional refuse-type destructor.

This report describes managerial aspects and briefly some technical accomplishments a Human Genome Database in Baltimore.

This report describes managerial aspects and briefly some technical accomplishments a Human Genome Database in Baltimore.

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This progress report describes gains made in three projects entitled (1) 3-Dimensional nuclear topography of genes and chromosomes in interphase nuclei, (2) Sequence specific identification and perturbation of the genomic DNA in living cells by nonionic oligonucleotide analogs (Matagen), and Resolution and isolation of specific DNA restriction fragments.(DT)

This progress report describes gains made in three projects entitled (1) 3-Dimensional nuclear topography of genes and chromosomes in interphase nuclei, (2) Sequence specific identification and perturbation of the genomic DNA in living cells by nonionic oligonucleotide analogs (Matagen), and Resolution and isolation of specific DNA restriction fragments.(DT)

The Geothermal Resources Interactive Temporal Simulation (GRITS) model is a computer code designed to estimate the costs of geothermal energy systems. The interactive program allows the user to vary resource, demand, and financial parameters to observe their effects on delivered costs of direct-use geothermal energy. Due to the large number and interdependent nature of the variables that influence these costs, the variables can be handled practically only through computer modeling. This report documents a sensitivity analysis of the cost of direct-use geothermal energy where each major element is varied to measure the responsiveness of cost to changes in that element. It is hoped that this analysis will assist those persons interested in geothermal energy to understand the most significant cost element as well as those individuals interested in using the GRITS program in the future.

In order to develop an initial estimate of the potential competitiveness of low temperature (45 degrees C to 100 degrees C) geothermal resources on the Eastern Coastal Plain, the Center for Metropolitant Planning and Research of The Johns Hopkins University reviewed and compared available energy price projections. Series of projections covering the post-1985 period have been made by the Energy Information Administration, Brookhaven National Laboratory, and by private research firms. Since low temperature geothermal energy will compete primarily for the space and process heating markets currently held by petroleum, natural gas, and electricity, projected trends in the real prices for these fuels were examined. The spread in the current and in projected future prices for these fuels, which often serve identical end uses, underscores the influence of specific attributes for each type of fuel, such as cleanliness, security of supply, and governmental regulation. Geothermal energy possesses several important attributes in common with electricity (e.g., ease of maintenance and perceived security of supply), and thus the price of electric space heating is likely to be an upper bound on a competitive price for geothermal energy. Competitiveness would, of course, be increased if geothermal heat could be delivered for prices closer to …

The processes involved in the formation of mixed oxides powders were studied using the counterflow diffusion flame burner. Powders of different morphologies were obtained by varying the flame conditions (temperature, residence time) and the concentration ratio of the oxides precursors. In-situ particle size and number density were determined using dynamic light scattering and 90{degrees} light scattering. A thermophoretic sampling method and a larger scale powder collection device also was used to collect particles, and their size and morphology examined using transmission electron microscopy, X-ray diffraction and surface area measurement by gas absorption (BET). Our emphasis has been on TiO{sub 2}-SiO{sub 2}, SiO{sub 2}-GeO{sub 2} and Al{sub 2}O{sub 3}-TiO{sub 2}. The powders had a core-mantle-like (one oxide coated by the other) at low elevations in the burner and uniform mixture at higher elevations. They form chain-like structures in a low temperature flame and spherical particles in a higher temperature flame. Nanometer sized homogeneous particles of Aluminum Titanate could be obtained using Al(CH{sub 3}){sub 3} and TiCl{sub 4} as precursors both in a hydrogen fueled and a methane fueled counterflow diffusion flame burner, as well as in a hydrogen fueled parallel-flow diffusion flame burner.

Letter from M. E. Andrews to Harris L. Kempner discussing general points of interest, such as Margeurite's condition, Jim McDonald's report on the Northern Ordnance, the Hazeltine problem, new Lieutenant Commanders and Lieutenants, his promotion and the weather in Washington.

Letter to NAACP leaders regarding voter registration drives. Letter encourages each chapter to set up registration sites and use their knowledge of their communities to get as many people registered as possible.

Memo to the youth departments regarding activities for the new year and updates on events and forms.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story about Arlington stadium where Rangers closed out their series against Kansas City with an 8- 3 win.

Photocopy of a script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story about the Rangers first big promotion of 1974 which was also a part of festivities at Arlington stadium.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story about the Texas rangers, top hitting team in the American league which opened a four game series with Minnesota.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story about the Kemper open at North Carolina which is the year' s second designated tournament and the sub par scores continued at an unbelievable rate.

Script from the WBAP-TV/NBC station in Fort Worth, Texas, relating a news story about the Texas Rangers who opened a two game series with the Kansas city royals in Kansas city.