The desirable features of cells in suspension will necessarily be dependent upon the use for which the cells were prepared. Adequate cell yield or recovery is defined by the measurement to be performed. Retention of cellular morphology is important for microscopic identification of cell types in a heterogenous cell suspension, and may be used to determine whether the cells in suspension are representative of those in the tumor in situ. Different dispersal protocols may yield cells with different degrees of clonogenicity, as well as altered biochemical features, such as loss of cellular proteins, surface antigens, nucleotide pools, etc. The quality of the cell suspension can be judged by the degree of cell clumping and level of cellular debris, both of which impact on flow cytometric measurements and studies in which the number of cells be known accurately. Finally, if the data measured on the cells in suspension are to be extrapolated to phenomena occurring in the tumor in situ, it is desirable that the cells in suspension are representative of those in the solid tumor in vivo. This report compares characteristics of tumor cell suspensions obtained by different types of selected disaggregation methods. 33 refs., 2 figs., 4 tabs.

The process of longitudinal compression of a drifting heavy ion pulse to be used as an ICF driver is examined with the aid of particle simulation. Space charge forces play a vital role in halting compression before the final focus lens system is reached. This must take place with minimal growth of transverse emittance and momentum spread. Of particular concern is the distortion of longitudinal phase space by the rounded transverse profile of the longitudinal self-electric field.

Corrosion of metals is an extremely important field with great economic and engineering implications at the Oak Ridge Y-12 Plant. To effectively combat corrosion, one must understand the processes occurring. This paper shows the utility of Secondary Ion Mass Spectrometry (SIMS) data for elucidating the processes occurring in one particular corrosion process - the oxidation of uranium by water - and for validating a theoretical model. It had long been known that the oxidation of uranium by water is retarded by the presence of oxygen gas and the retardation has been assumed to occur by site blocking at the surface. However, when alternate isotopic exposures were made, followed by exposure to a mixture of /sup 16/O/sub 2/ and /sup 18/OH/sub 2/, the rapid exchange of /sup 16/O and /sup 18/O occurred in the oxide layer, but the further oxidation by water in this and subsequent exposures was retarded for up to 21 hours. This shows graphically that OH/sub 2/ is not held up at the surface and that the retarding mechanism is effective at the oxide/metal interface rather than at the surface. The effectiveness of the O/sub 2/ to retard the further water oxidation was much reduced if no water-formed …

A major goal for commercial applications of fusion reactors is to minimize radioactive wastes and to dispose of them by near-surface burial. There currently are no regulations specifically applicable to fusion wastes but those in force for fission wastes furnish a framework for expected fusion regulations. This paper recommends that all nuclides with half-lives greater than five years be assigned concentration limits as done in 10CFR61 for fission wastes. The paper gives approximate limits for all the significant long half-life sources of gamma radiation in the currently known periodic table. In the absence of working fusion reactors, computer models must be used to estimate the expected actual concentrations of radioactive nuclides. These estimates are needed to guide design parameters to achieve minimum radioactivity in fusion reactors. It is believed that the computer models and nuclear reaction libraries must be much more comprehensive than ordinarily used today to do activation calculations.

Progress in laser fusion research has increased the need for detail and precision in the diagnosis of experiments. This has spawned the development and use of sophisticated sub-nanosecond resolution diagnostic systems. These systems typically use ultrafast x-ray or optical streak cameras in combination with spatially imaging or spectrally dispersing elements. These instruments provide high resolution data essential for understanding the processes occurring in the interaction of high intensity laser light with targets. Several of these types of instruments and their capabilities will be discussed. The utilization of these kinds of diagnostics systems on the nearly completed 100 kJ Nova laser facility will be described.

An article written by Steven Muller about the effects of newly changing research structures and disciplines at Johns Hopkins University.