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430 Matching Results
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DNA Damage Quantitation by Alkaline Gel Electrophoresis.
Physical and chemical agents in the environment, those used in clinical applications, or encountered during recreational exposures to sunlight, induce damages in DNA. Understanding the biological impact of these agents requires quantitation of the levels of such damages in laboratory test systems as well as in field or clinical samples. Alkaline gel electrophoresis provides a sensitive (down to {approx} a few lesions/5Mb), rapid method of direct quantitation of a wide variety of DNA damages in nanogram quantities of non-radioactive DNAs from laboratory, field, or clinical specimens, including higher plants and animals. This method stems from velocity sedimentation studies of DNA populations, and from the simple methods of agarose gel electrophoresis. Our laboratories have developed quantitative agarose gel methods, analytical descriptions of DNA migration during electrophoresis on agarose gels (1-6), and electronic imaging for accurate determinations of DNA mass (7-9). Although all these components improve sensitivity and throughput of large numbers of samples (7,8,10), a simple version using only standard molecular biology equipment allows routine analysis of DNA damages at moderate frequencies. We present here a description of the methods, as well as a brief description of the underlying principles, required for a simplified approach to quantitation of DNA damages by …
Date:
March 24, 2004
Creator:
Sutherland, B. M.; Bennett, P. V. & Sutherland, J. C.
Object Type:
Book
System:
The UNT Digital Library
Xradia Performance Summary - Update
High Energy Density Physics (HEDP) Experiments play an important role in corroborating the improved physics codes that underlie LLNL's Stockpile Stewardship mission. Conducting these experiments, whether on the National Ignition Facility (NIF) or another national facility such as Omega, will require not only improvement in the diagnostics for measuring the experiment, but also detailed knowledge of the as-built target components and assemblies themselves. To assist in this effort, a defined set of well-known reference standards designed to represent a range of HEDP targets have been built and are being used to quantify the performance of different characterization techniques. Without the critical step of using reference standards for qualifying characterization tools there can be no verification of either commercial or internallydeveloped characterization techniques and thus an uncertainty in the as-built-model for the initial condition to the physics codes.
Date:
May 24, 2004
Creator:
Waters, A M; Martz, H E; Chinn, D J; Logan, C M; Gross, J C & Scott, D
Object Type:
Report
System:
The UNT Digital Library
Degradation of Magnet Epoxy at NSLS X-Ray Ring.
Epoxy resin degradation was analyzed for NSLS X-ring magnets after two decades of 2.58-2.8 GeV continuous electron-beam operation, based on results obtained from thermoluminescent dosimeters irradiated along the NSLS ring and epoxy samples irradiated at the beamline target location. A Monte Carlo-based particle transport code, MCNP, was utilized to verify the dose from synchrotron radiation distributed along the axial- and transverse-direction in a ring model, which simulates the geometry of a ring quadrupole magnet and its central vacuum chamber downstream of the bending-magnet photon ports. The actual life expectancy of thoroughly vacuum baked-and-cured epoxy resin was estimated from radiation tests on similar polymeric materials using a radiation source developed for electrical insulation and mechanical structure studies.
Date:
May 24, 2004
Creator:
HU,J. P.; Zhong, Z.; Haas, E.; Hulbert, S. & Hubbard, R.
Object Type:
Article
System:
The UNT Digital Library
Defining and Testing the Influence of Servo System Response on Machine Tool Compliance
Compliance can be defined as the measurement of displacement per unit of force applied e.g. nano-meters per Newton (m/N). Compliance is the reciprocal of stiffness. High stiffness means low compliance and visa versa. It is an important factor in machine tool characteristics because it reflects the ability of the machine axis to maintain a desired position as it encounters a force or torque. Static compliance is a measurement made with a constant force applied e.g. the average depth of cut. Dynamic compliance is a measurement made as a function of frequency, e.g. a fast too servo (FTS) that applies a varying cutting force or load, interrupted cuts and external disturbances such as ground vibrations or air conditioning induced forces on the machine. Compliance can be defined for both a linear and rotary axis of a machine tool. However, to properly define compliance for a rotary axis, the axis must allow a commanded angular position. Note that this excludes velocity only axes. In this paper, several factors are discussed that affect compliance but emphasis is placed on how the machine servo system plays a key role in compliance at low to mid frequency regions. The paper discusses several techniques for measuring …
Date:
March 24, 2004
Creator:
Hopkins, D J
Object Type:
Article
System:
The UNT Digital Library
THERMODYNAMICS OF MATERIALS: FROM AB INITIO TO PHENOMENOLOGY
Quantum mechanical-based (or ab initio) methods are used to predict the stability properties of materials although their application is limited to relatively simple systems in terms of structures and number of alloy components. However thermodynamics of complex multi-component alloys requires a more versatile approach afforded within the CALPHAD formalism. Despite its success, the lack of experimental data very often prevents the design of robust thermodynamic databases. After a brief survey of ab initio methodologies and CALPHAD, it will be shown how ab initio electronic structure methods can supplement in two ways CALPHAD for subsequent applications. The first one is rather immediate and concerns the direct input of ab initio energetics in CALPHAD databases. The other way, more involved, is the assessment of ab initio thermodynamics '{acute a} la CALPHAD'. It will be shown how these results can be used within CALPHAD to predict the equilibrium properties of multi-component alloys. Finally, comments will be made on challenges and future prospects.
Date:
September 24, 2004
Creator:
Turchi, P A
Object Type:
Article
System:
The UNT Digital Library
Distributions of pI vs pH provide prior information for the design of crystallization screening experiments
None
Date:
August 24, 2004
Creator:
Kantardjieff, K A; Jamshidian, M & Rupp, B
Object Type:
Article
System:
The UNT Digital Library
Seismic Waves in Finely Layered VTI Media: Poroelasticity, Thomsen Parameters, and Fluid Effects on Shear Waves
Layered earth models are well justified by experience, and provide a simple means of studying fairly general behavior of the elastic and poroelastic characteristics of seismic waves in the earth. Thomsen's anisotropy parameters for weak elastic and poroelastic anisotropy are now commonly used in exploration, and can be conveniently expressed in terms of the layer averages of Backus. Since our main interest is usually in the fluids underground, it would be helpful to have a set of general equations relating the Thomsen parameters as directly as possible to the fluid properties. This end can be achieved in a rather straightforward fashion for these layered earth models, and the present paper develops and then discusses these relations. Furthermore, it is found that, although there are five effective shear moduli for any layered VTI medium, one and only one effective shear modulus for the layered system contains all the dependence of pore fluids on the elastic or poroelastic constants that can be observed in vertically polarized shear waves in VTI media. The effects of the pore fluids on this effective shear modulus can be substantial - an increase of shear wave speed on the order of 10% is shown to be possible …
Date:
February 24, 2004
Creator:
Berger, E. L.
Object Type:
Article
System:
The UNT Digital Library
Practical SQE on a Large Multi-Disciplinary HPC Development Team
In this paper we will discuss several software engineering practices that have proven useful in a large multidisciplinary physics code development project at Lawrence Livermore National Laboratory. In the project discussed here, as with many large scale efforts in HPC scientific computing, we have had to balance the competing demands of being a stable ''production'' code that our user base can rely on with being a platform for research into new physics, models, and software architectures. Much of this has been learned through necessity and experience. Likewise, much of it has been learned through interactions with other similar projects and hearing of their successes, and tailoring their ideas to our own requirements. The ideas presented here are not meant to necessarily transfer to other environments with different needs. It is our belief that projects need to be given large latitude in defining their own software engineering process versus a prescribed a solution. However, the ideas presented here are hopefully high level and general enough that we hope other projects might find some inspiration and adopt similar methods if it is to their benefit, much as we have done through the years.
Date:
March 24, 2004
Creator:
Neely, J R
Object Type:
Article
System:
The UNT Digital Library
X-ray Transport Optics and Diagnostics Commissioning Report
We discuss commissioning work funded through LCLS WBS element 1.5: X-ray Transport Optics and Diagnostics (XTOD.) A short description of the XTOD commissioning diagnostics hardware is followed by a brief discussion of FEL induced damage considerations. The remainder discusses simulation work on the response of the Direct Imager camera to a mix of spontaneous and FEL radiation and a Monte Carlo Calculation of the reflections of the spontaneous radiation in the undulator vacuum tube.
Date:
October 24, 2004
Creator:
Bionta, R M
Object Type:
Article
System:
The UNT Digital Library
Ensemble: 2004-02-24 – Spectrum
Access:
Use of this item is restricted to the UNT Community
Composition recital performed at UNT College of Music Recital Hall on February 24, 2004 at 8:00 pm.
Date:
February 24, 2004
Creator:
unknown
Object Type:
Sound
System:
The UNT Digital Library
The Colony Courier-Leader (The Colony, Tex.), Vol. 23, No. 7, Ed. 1 Wednesday, March 24, 2004
Weekly newspaper from The Colony, Texas that includes local, state, and national news along with advertising.
Date:
March 24, 2004
Creator:
Sorter, Dave
Object Type:
Newspaper
System:
The Portal to Texas History
The Colony Courier-Leader (The Colony, Tex.), Vol. 23, No. 42, Ed. 1 Wednesday, November 24, 2004
Weekly newspaper from The Colony, Texas that includes local, state, and national news along with advertising.
Date:
November 24, 2004
Creator:
Sorter, Dave
Object Type:
Newspaper
System:
The Portal to Texas History
The Alvin Sun (Alvin, Tex.), Vol. 114, No. 42, Ed. 1 Monday, May 24, 2004
Weekly newspaper from Alvin, Texas that includes local, state, and national news along with advertising.
Date:
May 24, 2004
Creator:
Schwind, Jim & Looby, Edward
Object Type:
Newspaper
System:
The Portal to Texas History
Needs Differing: Personality Dynamics for Peer Ombuds in a Research Setting
None
Date:
August 24, 2004
Creator:
Wolford, J. K.
Object Type:
Article
System:
The UNT Digital Library
Oral History Interview with William Hogg, January 24, 2004
The National Museum of the Pacific War presents an oral interview with William Hogg. Born in Louisiana 1 March 1926, Hogg joined the US Army Air Forces in 1944. After basic training at Amarillo, Texas he was selected for gunnery school and tells of training at various locations. Upon completion of gunnery training, he was assigned as a tail gunner on a B-24 bomber. He recalls that at 20 years of age the pilot and co-pilot were the oldest of the 10 crew members. Hogg boarded HMS Queen Elizabeth and sailed to Glasgow, Scotland, where he took a train to Norwich, England. There his crew was assigned to the 93rd Bomb Group. He flew no combat missions during the three months he was in England. Returning to the United States, he was discharged 2 May 1946.
Date:
January 24, 2004
Creator:
Hogg, William
Object Type:
Sound
System:
The Portal to Texas History
Correlates of Stress in People Living with HIV/AIDS
This poster suggests that a biopsychosocial approach may be beneficial to people living with HIV/AIDS to reduce stress.
Date:
March 24, 2004
Creator:
Ballinger, Julie & Vosvick, Mark A.
Object Type:
Poster
System:
The UNT Digital Library
Stigma, Coping, Pessimism, and Symptom Load: Covariates of Depression in Men and Women with HIV/AIDS
This poster investigates HIV-related self-stigma and prevalence of symptoms as stressors for people living with HIV.
Date:
March 24, 2004
Creator:
Ranucci, Melissa B. & Vosvick, Mark A.
Object Type:
Poster
System:
The UNT Digital Library
Oral History Interview with Thomas Alt, August 24, 2004
The National Museum of the Pacific War presents an interview with Thomas Alt. Alt joined the Aviation Cadet Corps in June of 1942 at the age of 17. By January of 1943, once he turned 18, he was called up for training. In mid-1943 he traveled to Pearl Harbor, and completed Fire Fighting School with the Navy while on the island. In mid-1944 Alt was assigned to the USS Sea Cat (SS-399). He worked as Military Police on night shifts and guard duty in the brig. They traveled to Saipan and the South China Sea where the Sea Cat operated in a wolf pack. He landed on Saipan 7 July 7, as the battle was coming to an end. He served with a firefighting crew on the beach for pilots making emergency or crash landings. Alt remained on Saipan through early 1945. He was stationed on Tinian in July, and recalls when the Enola Gay landed. He returned to the US and was discharged in March of 1945.
Date:
August 24, 2004
Creator:
Alt, Thomas
Object Type:
Sound
System:
The Portal to Texas History
Oral History Interview with Daniel Clark, June 24, 2004
The National Museum of the Pacific War presents an oral interview with Daniel Clark. Clark was born in Morocco, Indiana on 26 June 1916. After graduating from high school in 1934, he worked on the farm until being drafted into the US Army in March 1942. Upon induction, he was sent to Camp Claiborne, Louisiana for six months of basic training. He was then sent to Ft. Bragg, North Carolina where he joined the 82nd Airborne Division’s glider section and was assigned to the 325th Glider Infantry, 2nd Battalion, Company F. Upon completion of training the unit boarded the SS Santa Rosa for a twelve day trip to Casa Blanca, North Africa. His narrative is a well told tale of his participation in Operation Overlord, Operation Market Garden and the Battle of the Bulge and of the horrific scene found when the unit liberated the Wobbelin Concentration Camp on 5 May 1945.
Date:
June 24, 2004
Creator:
Clark, Daniel C.
Object Type:
Sound
System:
The Portal to Texas History
Oral History Interview with Fred Zellers, May 24, 2004
The National Museum of the Pacific War presents an interview with Fred Zellers. Zellers joined the Navy in March of 1944 and was assigned to the Armed Guard. In August he began serving aboard the SS Oremar (1919), a merchant ship. They transported 500- and 1,000-pound bombs with a convoy to England. Zellers worked as a Gunner’s Mate and mathematical calibration specialist on the ship, and they shuttled bombs between England, France, Belgium and Holland. They returned home in December of 1944. In 1945, they continued transporting supplies to France and Belgium, including officers’ footlockers and food. Zellers was discharged in October of 1945.
Date:
May 24, 2004
Creator:
Zellers, Fred
Object Type:
Sound
System:
The Portal to Texas History
Natural Analogue Synthesis Report
None
Date:
May 24, 2004
Creator:
Simmons, A. M.
Object Type:
Report
System:
The UNT Digital Library
NA22 Model Cities Project - LL244T An Intelligent Transportation System-Based Radiation Alert and Detection System
The purpose of this project was twofold: first, provide an understanding of the technical foundation and planning required for deployment of Intelligent Transportation System (ITS)-based system architectures for the protection of New York City from a terrorist attack using a vehicle-deployed nuclear device; second, work with stakeholders to develop mutual understanding of the technologies and tactics required for threat detection/identification and establish guidelines for designing operational systems and procedures. During the course of this project we interviewed and coordinated analysis with people from the New Jersey State Attorney General's office, the New Jersey State Police, the Port Authority of New York/New Jersey, the Counterterrorism Division of the New York City Police Department, the New Jersey Transit Authority, the State of New Jersey Department of Transportation, TRANSCOM and a number of contractors involved with state and federal intelligent transportation development and implementation. The basic system architecture is shown in the figure below. In an actual system deployment, radiation sensors would be co-located with existing ITS elements and the data will be sent to the Traffic Operations Center. A key element of successful system operation is the integration of vehicle data, such as license plate, EZ pass ID, vehicle type/color and radiation …
Date:
February 24, 2004
Creator:
Peglow, S
Object Type:
Report
System:
The UNT Digital Library
Oak Ridge Reservation Annual Site Environmental Report, 2003
This document is prepared annually to summarize environmental activities, primarily environmental-monitoring activities, on the ORR and within the ORR surroundings. The document fulfills the requirement of U.S. Department of Energy (DOE) Order 231.1, ''Environment, Safety and Health Reporting,'' for an annual summary of environmental data to characterize environmental performance. The environmental monitoring criteria are described in DOE Order 450.1, ''Environmental Protection Program''. The results summarized in this report are based on data collected prior to and through 2003. This report is not intended to provide the results of all sampling on the ORR. Additional data collected for other site and regulatory purposes, such as environmental restoration remedial investigation reports, waste management characterization sampling data, and environmental permit compliance data, are presented in other documents that have been prepared in accordance with applicable DOE guidance and/or laws. Corrections to the report for the previous year are found in Appendix A. Environmental monitoring on the ORR consists primarily of two major activities: effluent monitoring and environmental surveillance. Effluent monitoring involves the collection and analysis of samples or measurements of liquid and gaseous effluents at the point of release to the environment; these measurements allow the quantification and official reporting of contaminants, assessment …
Date:
August 24, 2004
Creator:
Hughes, JF
Object Type:
Report
System:
The UNT Digital Library
Factors Affecting Zebra Mussel Kill by the Bacterium Pseudomonas fluorescens
The specific purpose of this research project was to identify factors that affect zebra mussel kill by the bacterium Pseudomonas fluorescens. Test results obtained during this three-year project identified the following key variables as affecting mussel kill: treatment concentration, treatment duration, mussel siphoning activity, dissolved oxygen concentration, water temperature, and naturally suspended particle load. Using this latter information, the project culminated in a series of pipe tests which achieved high mussel kill inside power plants under once-through conditions using service water in artificial pipes.
Date:
February 24, 2004
Creator:
Molloy, Daniel P.
Object Type:
Report
System:
The UNT Digital Library