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Creation and Characterization of an Escherichia Coli and Pseudomonas Putida Hybrid Aspartate Transcarbamoylase
Aspartate transcarbamoylase (ATCase) is encoded by the pyrBI genes in E. coli. Expression of these genes is reduced four-fold by attenuation when grown on uracil. Using plasmid, pRO1727. the pyrB and the pyrBI genes from E. coli were cloned into a P. putida pyrB auxotroph. A recombinant pyrB gene was recovered that encoded a functional hybrid ATCase with a molecular weight of 470 kDa.
Date:
December 1992
Creator:
Ruley, Jill R. (Jill Rosanne)
System:
The UNT Digital Library
The GABAa Receptor in the Central Nervous System of a Rat Model of Epilepsy
This study investigated the β-subunit of the GABAa receptor and determine if there are changes in the primary sequence of the extracellular N-Terminal domain, which likely regulates GABA binding.
Date:
August 1992
Creator:
Sherman, John Mark
System:
The UNT Digital Library