Racial Microaggressions, Racial/Ethnic Identity, and Sense of Belonging among Students of Color

Victims of racial discrimination often experience negative consequences that extend into all aspects of well-being (e.g., psychological, subjective, social). Racial microaggressions describe a series of verbal and non-verbal behaviors that cause harm, perpetuate negative stereotypes, and negate the experience of racial/ethnic minorities. Research has found a negative relationship between experiencing racial discrimination and psychological well-being, as well as a potential buffering effect of racial/ethnic identity. However, less information is available about the existence of these relationships with social well-being. The purpose of this dissertation is to fill a gap in the literature in regard to racial microaggressions, racial/ethnic identity, and social well-being in 453 racially diverse undergraduate students. Quantitative results indicated that African Americans reported higher levels of racial microaggressions and racial/ethnic identity than other groups, racial microaggressions were negatively associated with sense of belonging, racial/ethnic identity was positively associated with sense of belonging, and the association between racial microaggressions and sense of belonging was not moderated by racial/ethnic identity. Qualitative analyses using the conventional content analysis revealed two clusters of facilitative behavioral expressions of university/community inclusion: (a) interpersonal expressions of inclusion and (b) systemic expression of inclusion. I conclude by discussing limitations, areas for future research, and implications for …
Date: August 2022
Creator: Davis, Cameron W
System: The UNT Digital Library

The Influence of the Consolidated Appropriations Act of 2012 on Pell Grants in Baccalaureate Degree-Granting Institutions

The Consolidated Appropriations Act of 2012 (CAA) was an omnibus spending bill that changed Pell grant funding in response to a budgetary shortfall after a four-year investment in student financial aid. The CAA occurred at a time of state disinvestment coupled with increasing tuition and fees. Through the lens of resource dependence and academic capitalism as a conceptual framework, I used panel data in an ordinary least squares (OLS) regression to explore the impact of the CAA on Pell grant revenues. I included an interaction term to specifically address the impact of the CAA on public regional universities (PRUs) versus other institutions. Results indicated that the interaction effect between CAA and PRU was jointly significant. The average Pell award per student at PRUs was $13 less than other institutions before the implementation of the CAA. The average Pell award per student at PRUs increased after the implementation of the CAA; however, the average Pell award per student at PRUs was still $20 less than other institutions. Results indicated that the percentage of students receiving Pell awards was a significant and positive predictor of an average Pell award. Interestingly, institutions designated as minority-serving institutions (MSIs) were also significant and positive predictors …
Date: May 2022
Creator: Firmin, Tara
System: The UNT Digital Library
Cross Validation of the Juror Questionnaire of Values and Viewpoints: Sentencing Decisions and Impression Management in Eligible Capital Jurors (open access)

Cross Validation of the Juror Questionnaire of Values and Viewpoints: Sentencing Decisions and Impression Management in Eligible Capital Jurors

The current dissertation had three primary objectives, categorized into two MTurk studies with capital juror-eligible community members: (a) cross-validate the psychometric properties of the JQVV, (b): explore the role of legal attitudes via the JQVV in mock capital sentencing decisions, and (c): examine the JQVV's ability to detect juror social desirability in capital voir dire. Impressively, Study 1 (N = 552) and Study 2 (N = 313) provided strong and consistent evidence for the JQVV's reliability and construct validity. In the mock juror paradigm, punitive legal attitudes on the JQVV (i.e., Crime-Neg, Convict, and Death-Pos), did not directly affect sentencing decisions, however they indirectly influenced the perception of nearly all other legally relevant variables (e.g., evidence type). For example, participants with more punitive criminal justice attitudes evaluated aggravating evidence more favorably which, in turn, increased death sentence verdicts. Study 1 also underscored the concerningly low levels of comprehension jurors have regarding judicial instructions and other relevant legal knowledge (e.g., the definition of aggravating). In Study 2, the support-life and support-death groups evidenced divergent patterns of social desirability, although support-death participants did not dramatically alter their scores between the genuine and social desirability condition. Additionally, the JQVV Pros-Cyn and Justice-Pos scales …
Date: August 2022
Creator: Hartigan, Sara E
System: The UNT Digital Library
Executive Functioning Processes in Simple and Complex Theory of Mind Tasks (open access)

Executive Functioning Processes in Simple and Complex Theory of Mind Tasks

Using a multimethod-multimodal approach, this study compared the contributions of executive function (EF) abilities (Go No-Go, Visual Search, 2-Back task, and Task Switching) to narrative comprehension tasks (False Belief, Strange Stories, Self-Reported Theory of Mind Inventory [TOMI-SR]) and a narrative production task (interpersonal decentering) in a sample of young adults. Separate regression models were conducted for each theory of mind (ToM) measure with EF measures as predictor variables and empirically selected demographic variables controlled. As expected, in this college student sample (N = 110), False Belief demonstrated a ceiling effect and was not associated with any EF ability. Task Switching and 2-Back accounted for significant variance in Strange Stories. No EF task significantly predicted performance on TOMI-SR or interpersonal decentering. Both story comprehension tasks (False Belief and Strange Stories) were significantly associated, but these tasks were not correlated with either self-reported ToM or interpersonal decentering. Several unanticipated demographic associations were found; having more siblings and English proficiency accounted for significant variability in Strange Stories; education, presence or absence of self-disclosed autism diagnosis and mental health diagnosis explained a large portion of variance in TOMI-SR; interpersonal decentering maturity differed significantly between cisgender men and cisgender women. Lastly, interpersonal decentering number of …
Date: August 2022
Creator: Shamji, Jabeen Fatima
System: The UNT Digital Library

Death Awareness and Meaningful Work: Considering Mortality and How It Relates to Individual Perceptions of Work

While some individuals experience their work as meaningful, others, with the same job, do not. The purpose of this dissertation is to answer the following question: Why do different individuals, with the same job, view the meaningfulness of their work in conflicting ways? I draw on terror management theory and generativity theory to answer this question by testing the relationship between death awareness and meaningful work. The bulk of academic work concerning meaningful work focuses on its outcomes and few scholars have explained the antecedents of meaningful work. This study aims to extend empirical work of the relationship between death awareness and meaningful.
Date: August 2022
Creator: Varghese, Johnson George
System: The UNT Digital Library
Middle Childhood Behavioral Attachment Dimensions (MCBAD):  Development and Validation of an Observational System for Coding Dimensions of Attachment Security in Middle Childhood (open access)

Middle Childhood Behavioral Attachment Dimensions (MCBAD): Development and Validation of an Observational System for Coding Dimensions of Attachment Security in Middle Childhood

Middle childhood is the least understood developmental period in the attachment literature, likely due to active reorganization of the attachment system during this stage. Coinciding with increased cognitive and socioemotional competencies, middle childhood attachment begins to transition from relationship-specific attachment to general attachment representations. While parents continue serving as the primary attachment figure used as a secure base and safe haven, noticeable shifts occur in terms of the need for availability over proximity to attachment figures and the child's involvement in maintaining the attachment relationship. Currently, there is no dominant conceptual or methodological approach for studying attachment in middle childhood. The present study sought to develop and validate an observational coding system examining middle childhood attachment using a dimensional approach. The Middle Childhood Behavioral Attachment Dimensions (MCBAD) system demonstrated mixed reliability and validity compared to other established attachment measures. Compared to traditional categorical attachment measures, this study provides evidence for the utility of an anxiety-avoidance dimensional attachment scale in childhood. Additionally, the MCBAD is the first observational system that examines both nonverbal and verbal attachment behaviors in an unstructured separation-reunion episode, and findings suggest a need for further examination of verbal attachment during this developmental stage.
Date: August 2022
Creator: Geerts-Perry, Ashley
System: The UNT Digital Library

A Multi-Level Model for Perception Affect Asymmetry: Individual, Dyadic, and Group Affect Dynamics

In collective affect research, an assumption is often made that through processes such as emotional contagion and attraction-selection-attrition members will converge unto a shared group affective tone. While this assumption is not without warrant, a limitation of previous work on interpersonal emotional processes of individuals, individuals within dyadic relationships, or members within teams is the lack of examination into the varying perceptions individuals may form regarding these affective experiences. To examine the development and influence of these affective perceptions, we extend recent works from work group conflict literature to examine the influence of perception asymmetry when applied to affective interactions. Wherein, we describe a novel construct of Perception Asymmetry of Affect (PAA). PAA refers to the congruence (e.g.; low level of PAA) or incongruence (e.g.; high level) of perceptions of positive and negative affective experiences between two or more individuals. This paper explores the following questions: 1) does perception asymmetry of affect exist; 2) if so, what causes perception asymmetry between individuals and their groups, within dyads, and within groups. This article contributes to literature on collective affect by offering a detailed framework for an understudied phenomenon of diverging or asymmetric perceptions.
Date: December 2022
Creator: Antwiler, Brandon
System: The UNT Digital Library
A Comparison of Plaintiff and Defense Expert Witness H-Index Scores in Mild Traumatic Brain Injury Civil Litigation (open access)

A Comparison of Plaintiff and Defense Expert Witness H-Index Scores in Mild Traumatic Brain Injury Civil Litigation

This study examines the background and qualifications of plaintiff and defense experts using the H-Index score as quantification of expert background and qualifications. The goal is to better understand the similarities and differences among the professionals offering paid expert witness testimony in mild traumatic brain injury (mTBI) civil litigation. In this quantitative study, descriptive statistics include the mean and standard deviation scores for the data to support examining measures of central tendency and variance, respectively. The study includes the use of logistic regression and the Wilcoxon signed-rank test, and their statistical assumptions were tested to determine whether they would be used or if it was more appropriate to use a non-parametric test. The study included two research questions: How do the qualifications of plaintiff and defense expert witnesses in mild traumatic brain injury civil litigation compare? and to what extent does a higher h-index correlate with a favorable litigation outcome in a mild traumatic brain injury case? The findings for the hypothesis tests associated with the research questions led to the acceptance of the null hypothesis in each test. There was a lack of asymptotic significance in Hypothesis 1 and a lack of significance in Hypothesis 2. The findings from …
Date: August 2022
Creator: Victor, Elise C
System: The UNT Digital Library
Big Game, Big Decisions, and Big Government: Understanding the Effects of Commodification on Deer and Feral Hog Hunting in Texas (open access)

Big Game, Big Decisions, and Big Government: Understanding the Effects of Commodification on Deer and Feral Hog Hunting in Texas

My research examines how primary stakeholders interact with Texas' most harvested big game animals: white-tailed deer, which are increasingly impacted by chronic wasting disease (CWD), and feral hogs, which impact the landscape but effectively have no management strategy. Drawing on literature on wildlife governance in Texas, managing property and the commons, and disease landscapes, and broadly framed by themes of political and historical ecology, my research asks: (1) how do management goals for deer and feral hogs compare to hunting practices and hunting culture in Texas? (2) How are deer commodified by the Texas deer breeding industry? (3) How does the commodification of deer by breeders impact deer hunting practices in Texas? To examine how local stakeholders manage CWD and feral hogs, I conducted interviews among 21 stakeholders, including hunters, game wardens, game ranch managers, and deer breeders in Texas, as well as conducting participant observation at three deer conferences. Analysis shows that contrary to my expectations, not all participants viewed feral hogs negatively, with some viewing them as profit-making ventures. Inversely, how stakeholders contend with and understand CWD varies by a stakeholder's ability to generate profit from deer breeding. Furthermore, the majority of participants identified deer breeding operations as …
Date: December 2022
Creator: Tabor, Zachary Dalton
System: The UNT Digital Library
Performing Translations: Rethinking Christian Wolff's Alternative Notation (1960-1968) in the Context of His Creative Communities (open access)

Performing Translations: Rethinking Christian Wolff's Alternative Notation (1960-1968) in the Context of His Creative Communities

Christian Wolff's alternatively notated scores grant the performer several interpretive choices. These pieces feature symbols (known as "coordination neumes") that instruct performers when to begin and end a sound event in relation to the sounds being made around them, thereby generating a reactive improvisation between the musicians. Among these scores are five compositions that form the basis of this project: For 5 or 10 People (1962), In Between Pieces (1963), For 1, 2, or 3 People (1964), Septet (1964), and Edges (1968). Focusing on these pieces specifically, this dissertation explores the unique performance practices required by Wolff's indeterminate music and contextualizes that music within his career in classics and comparative literature, particularly with regard to the concept of translation, and within his creative communities. These creative communities include his fellow New York School composers, New York's wider downtown artistic scenes in the 1950s and 60s, and the experimental music scenes at Cologne and Darmstadt. While scholars such as David Behrman, Thomas DeLio, and Mark Nelson have addressed the interactive quality of Wolff's notation and the technical skills needed to execute his pieces, I argue that there are deeper processes at work in these compositions that go beyond typical discussions of …
Date: December 2022
Creator: Stearns, Jessica
System: The UNT Digital Library

Adoption of Wearable Devices by Older Adults

This dissertation is organized in a traditional format while including three essays that address specific research questions. Essay 1 examined the relationship between physical activity and community engagement and their effect on mental well-being among older men and women. Data from National Health and Aging Trends Study (NHATS) from 2018 to 2020 were explored and the posited relationships were tested. This essay provides empirical support that older adults who are reasonably active and involved in the community have greater mental well-being than those who isolate themselves. Essay 2 provides insight into older adults' motivation to improve their physical activity through the use of a fitness tracker. The key finding from this study is that wearables, especially fitness trackers, can significantly facilitate increased physical activity. Essay 3 is a mixed-methods study to understand older adults' perception of the usefulness of fitness trackers and interaction with such devices. Findings suggest that to increase the adoption of fitness trackers among older adults, makers could improve the esthetics and quality of the wristband in addition to the battery life of the tracker.
Date: May 2022
Creator: Enamela, Pranathy
System: The UNT Digital Library

Students' and Teachers' Perceptions of Mathematics through Their Lived Experiences in Classrooms and Communities

This dissertation includes background on influences of mathematics, mathematics education, and who is viewed as a mathematician leading into three articles exploring students' and teachers' perceptions of mathematics through their lived experiences in both mathematics classrooms and their communities. Using interpretative phenomenological analysis for the methodology, all three articles analyze mathematics autobiographies and semi-structured interviews with five student participants enrolled in the same Algebra I course; Paper 3 also includes the Algebra I teacher. Paper 1 focuses on how students describe their lived experiences in mathematics classrooms. Three themes emerged from the participant data: 1) lack of autonomy and access, 2) feelings hinge on performance in mathematics, and 3) the need for support in mathematics. Each participant shared different experiences, but these experiences can help inform educators how to improve students' experiences in the classroom. Paper 2 sought to understand how middle grade students make sense of what it means to do mathematics in their community. The three themes include: 1) navigating the usefulness of mathematics outside of school, 2) who directs mathematics outside of school, and 3) the need for mathematics in future plans. Connections students made between mathematics and the lives outside of school varied suggesting how broad …
Date: August 2022
Creator: Hulme, Keely
System: The UNT Digital Library

Measuring Creativity in Academic Writing

The demand for a creative workforce has never been higher, yet schools struggle to teach and assess creativity among students predictably and efficiently. Compositions are an effective way to incorporate creativity across the curriculum; however, essays are time consuming for teachers to score for objective quality or subjective creativity. In this study, I explored a) if high creativity scores are related to high quality and sophistication in academic writing, and b) if extant text-mining tools effectively identify quality, sophistication, and creativity in academic essays. I collected 230 essays written by Grade 11 students. Four human-raters analyzed these essays for quality, sophistication, and creativity, and I used text-mining tools designed to assess creativity to analyze the same. Using correlations - including the variables semantic distance (measured against the GloVe corpus), entropy (measured with Shannon's Entropy Index), and idea density (measured with CPIDR5.1) - I assessed both human-raters' and text-mining tools' proficiency at identifying quality, sophistication, and creativity in academic essays. Quality, sophistication, and creativity were also regressed on these same text-mining variables to assess which method - human or computer – and which of the text-mining tools - best predicts these dependent variables. Human-raters' creativity scores correlated with human-raters' quality scores …
Date: December 2022
Creator: Nagel, Janessa Helen Bower
System: The UNT Digital Library
Including Special Education Teachers in High Functioning Professional Learning Communities: Implications for School Leaders (open access)

Including Special Education Teachers in High Functioning Professional Learning Communities: Implications for School Leaders

Public education in America became a target of reform since the passage of Brown v. Board of Education of Topeka. Some reforms come from court cases, some from presidents wanting to enact change, and others from political events from as far away as Russia. Yet, one concept remains the same: the improvement of educators is needed for supporting student growth and achievement. Getting both special education and general education teachers together to work collaboratively is critical to the growth of teachers and students. A gap in education research exists in the area of including special education teachers in high functioning profession learning communities (PLCs). The purpose of this study was to examine the impact that special education teachers have on students and other teachers when special education teachers are included in content-area PLCs. Using a mixed method case study design, quantitative data from an electronic survey and qualitative data from face-to-face interviews and PLC documents were collected and analyzed to answer two research questions. The survey findings revealed that the studied district operates high functioning PLC teams. One-on-one, semi-structured interviews and PLC document analysis showed that special education teachers were included on a regular basis in PLCs in three of …
Date: December 2022
Creator: Wilshire, John Mark
System: The UNT Digital Library

Change AGENT Project Part 2: Further Analyses of Progress Following Staff Training on Responsive, Goal-Directed, and Rationale-Based Decision Making

Evidence-based practice in ABA is a complex decision-making process involving frequent adjustments in goals and procedures as informed by science, client need, and clinical wisdom. Consistent with the science's foundations, incredible gains are possible for children with autism when practitioners are systematically trained to understand, produce, and be responsive to shifting conditions for change. However, minimal standards for training promote inflexibility and rule following, at the expense of frequent and responsive adjustments. Although research has demonstrated that well-trained staff can effectively implement flexible procedures using in-the-moment assessment and clinical judgment, minimal research has targeted and evaluated the development of these repertoires. Therefore, the purpose of this study was to evaluate the effects of a staff training package, including an in-service training and in vivo training sessions, on staffs' ability to make responsive, goal-directed, and rationale-based decisions directed towards accelerating progress with vocal manding for children with autism. The evaluation was the second part of the larger Change AGENT Project. Results suggested the training was effective at producing socially validated progress across staff and child measures. In essence, the trainer, staff, and child acted as change agents for one another's behavior within the flexible paradigm. Implications, limitations, and future directions are …
Date: December 2022
Creator: Schleifer-Katz, Evan
System: The UNT Digital Library

Increasing the Effectiveness of Location-Based Advertising

Location-based ads are defined as any ads that are sent by an identified source to consumers' mobile devices when they are around the advertised product/store. Although mobile ad spending is said to have accounted for 68% of all digital ad spending in 2020, knowledge of how businesses can use mobile and location-based technology to reach their customers effectively is limited. Thus, the purpose of this three-essay format dissertation is to review the literature on LBA and identify the gaps in the literature and attempt to address a few of these gaps in the remaining two essays. The second essay tests an integrated model by examining the effects of the various retailer and consumer-controlled factors on two retail performances. In the third essay, we explore how LBA affects different dimensions of consumer-brand engagement. The results and findings provide important implications for retailers and brands to increase their performances.
Date: August 2022
Creator: Thapa, Sajani
System: The UNT Digital Library

Ultrasonic Wave Propagation and Localization in a Nonreciprocal Phononic Crystal

Ultrasonic wave propagation through a two-dimensional nonreciprocal phononic crystal with asymmetric aluminum rods in viscous water is studied for its application in Anderson localization and trapping of acoustic energy. A one-dimensional disorder in the otherwise 2D periodic crystal is introduced by disorienting the asymmetric rods along the rows and by keeping them equally oriented along the columns. An exponential decay of sound waves travelling along the direction of disorder is observed demonstrating Anderson localization whereas sound propagates as extended wave along the ordered direction. Localization length for the case of strong disorder with high randomness in the orientation of rods and weak disorder with weak fluctuations in the orientation of rods is evaluated. The degree of randomness in the orientation of the rods controls the localization length of the wave. Thouless's theoretical prediction for the scaling of Lyapunov exponent with disorder is experimentally observed for weak disorder at frequency in the transmission band and anomalous scaling is observed for band edge frequency. Transmission spectra of acoustic waves is also measured for opposite direction of propagation and nonreciprocity is observed for the exponentially weak transmission in the disordered direction as well as for extended states in the ordered direction. Breaking of …
Date: December 2022
Creator: Dhillon, Jyotsna
System: The UNT Digital Library

Understanding and Reasoning with Negation

In this dissertation, I start with an analysis of negation in eleven benchmark corpora covering six Natural Language Understanding (NLU) tasks. With a thorough investigation, I first show that (a) these benchmarks contain fewer negations compared to general-purpose English and (b) the few negations they contain are often unimportant. Further, my empirical studies demonstrate that state-of-the-art transformers trained using these corpora obtain substantially worse results with the instances that contain negation, especially if the negations are important. Second, I investigate whether translating negation is also an issue for modern machine translation (MT) systems. My studies find that indeed the presence of negation can significantly impact translation quality, in some cases resulting in reductions of over 60%. In light of these findings, I investigate strategies to better understand the semantics of negation. I start with identifying the focus of negation. I develop a neural model that takes into account the scope of negation, context from neighboring sentences, or both. My best proposed system obtains an accuracy improvement of 7.4% over prior work. Further, I analyze the main error categories of the systems through a detailed error analysis. Next, I explore more practical ways to understand the semantics of negation. I consider …
Date: December 2022
Creator: Hossain, Md Mosharaf
System: The UNT Digital Library

Response to Regulation of Technology: A Multi-Industry Perspective

Overall my dissertation work tries to capture a holistic view of the various complex interactions that occur in technology development, implementation, adoption and diffusion, in the context of three industries by examining issues that arise due to regulation of technology. Essay 1 focuses on the social media industry, which is in the early stage of the industrial life cycle, and is the foci of government attention for its ill effect on society. Results from the study (N= 647 employed adults in the US) supported hypotheses related to the antecedents and outcomes of platform utilization in the context of the three regulation dimensions. Essay 2 focuses on the automotive industry, which is in the growth stage of the industrial life cycle. Here the focus is on electric vehicles (EV) transitioning from the niches to the main market. Results from the longitudinal study (N = 429) support the moderating role of political activism on innovation capability of manufacturers and presence of ancillary services in the diffusion of different types of electric vehicles in the US market. Essay 3 focuses on the US healthcare industry, reflecting mature stage of industrial life cycle, yet also characterized with high cost and fragmentation of service. The …
Date: August 2022
Creator: Bhawal, Shalini
System: The UNT Digital Library
An Uncivil Student and an Antagonistic Professor Walk into a Classroom: How Instructor Behavior During Class Conflict Impacts Learning (open access)

An Uncivil Student and an Antagonistic Professor Walk into a Classroom: How Instructor Behavior During Class Conflict Impacts Learning

Exceptional classroom management (CM) for face-to-face and online classes is vital to instructor success, and importantly, directly impacts students' ability to learn. Classroom conflict may disrupt an instructor's CM and can occur when a student is uncivil (e.g., sidetracks from lecture) or when an instructor misbehaves (e.g., antagonizes students). A small but meaningful line of work suggests that uncivil students and misbehaving teachers negatively impact the learning environment. However, no work has examined how the interaction between an uncivil student and misbehaving teacher impacts learning. As such, the purpose of the current study is to empirically investigate how teacher responses to student incivility impact cognitive learning in an online learning environment. The project evaluated approximately 252 undergraduate students via an online study. Participants watched a video of an online class in which the professor responds to an uncivil student in one of three different ways: antagonistically, positively, or neutrally. Participants then took a cognitive learning quiz based on the lecture and answered questions about their perception of the instructor, uncivil student, and the learning environment. Results of the one-way ANOVA suggest that how an instructor responded to student incivility did not significantly impact cognitive learning. Secondary analyses also indicated that …
Date: December 2022
Creator: Carey, Caitlyn Nicole
System: The UNT Digital Library

Ecosystem Services and Sustainability: A Framework for Improving Decision-Making in Urban Areas

Ecosystem services are the varied goods and benefits provided by ecosystems that make human life possible. This concept has fostered scientific explorations of the services that nature provides to people with the goal of sustaining those services for future generations. As the world becomes increasingly urban, ecosystems are reshaped, and services are degraded. Provisioning and regulating ecosystem services, landscape planning, decision making, and agricultural systems and technologies play a distinctive role in feeding and sustaining the expanding urban population. Hence, the integrated assessment of these coupled components is necessary to understand food security and sustainable development. Nevertheless, frameworks that incorporate ecosystem services, urbanization, and human wellbeing are still scarce due to several conceptual and methodological gaps that challenge this assessment. As a consequence, these frameworks are not operationalized, and ecosystem services rarely receive proper attention in decision making. This dissertation seeks to improve our understanding of the role of ecosystem services at the landscape level and provides an approach for operationalizing decisions that affect sustainable practices and human wellbeing.
Date: May 2022
Creator: Valencia Torres, Angélica
System: The UNT Digital Library

Developing a Generalizable Two-Input Genetic AND Logic Gate in Arabidopsis thaliana for Multi-Signal Processing

With effective engineering using synthetic biology approaches, plant-based platforms could conceivably be designed to minimize the production costs and wastes of high-value products such as medicines, biofuels, and chemical feedstocks that would otherwise be uneconomical. Additionally, modern agricultural crops could be engineered to be more productive, resilient, or restorative in different or rapidly changing environments and climates. To achieve these complex goals, information-processing genetic devices and circuits containing multiple interacting parts that behave predictably must be developed. A genetic Boolean AND logic gate is a device that computes the presence or absence of two inputs (signals, stimuli) and produces an output (response) only if both inputs are present. Here, we optimized individual genetic components and used synthetic protein heterodimerizing domains to rationally assemble genetic AND logic gates that integrate two hormonal inputs in whole plants. These AND gates produce an output only in the presence of both abscisic acid and auxin, but not when either or neither hormone is present. Furthermore, we demonstrate the AND gate can also integrate two plant stresses, cold temperature and bacterial infection, to produce a specific response. The design principles used here are generalizable, and therefore multiple orthogonal AND gates could be assembled and rationally …
Date: December 2022
Creator: Anderson, Charles Edgar
System: The UNT Digital Library
Acute Toxicity of Crude Oil Exposures to Early Life Stage Teleosts: Contribution of Impaired Renal Function and Select Environmental Factors (open access)

Acute Toxicity of Crude Oil Exposures to Early Life Stage Teleosts: Contribution of Impaired Renal Function and Select Environmental Factors

Oil spills are well-known adverse anthropogenic events, as they can induce severe impacts on the environment and negative economic consequences. Still, much remains to be learned regarding the effects of crude oil exposure to aquatic organisms. The objectives of this dissertation were to fill some of those knowledge gaps by examining the effects of Deepwater Horizon (DWH) crude oil exposure on teleost kidney development and function. To this end, I analyzed how these effects translate into potential osmoregulatory impairments and investigated the interactive effects of ubiquitous natural factors, such as dissolved organic carbon (DOC) and ultraviolet (UV) light, on acute crude oil toxicity. Results demonstrated that acute early life stage (ELS) crude oil exposure induces developmental defects to the primordial kidney in teleost fish (i.e., the pronephros) as evident by alterations in: (1) transcriptional responses of key genes involved in pronephros development and function and (2) alterations in pronephros morphology. Crude oil-exposed zebrafish (Danio rerio) larvae presented defective pronephric function characterized by reduced renal clearance capacity and altered filtration selectivity, factors that likely contributed to the formation of edema. Latent osmoregulatory implications of crude oil exposure during ELS were observed in red drum (Sciaenops ocellatus) larvae, which manifested reduced survival …
Date: August 2022
Creator: Bonatesta, Fabrizio
System: The UNT Digital Library
The Emergence of All-State Vocal Jazz Ensembles in the United States from 1978 to 2022 (open access)

The Emergence of All-State Vocal Jazz Ensembles in the United States from 1978 to 2022

Since the creation of the first all-state vocal jazz ensemble in 1978, similar ensembles have been established in roughly half of the United States. This paper contains historical summaries of the creation of all-state vocal jazz ensembles in nineteen of those states, primarily as recounted via interviews with those ensembles' founders. Each semi-structured interview was conducted over video conference or phone and lasted approximately one hour; resulting interview data was analyzed using qualitative methods. During the creation of each ensemble the respective founders needed to secure the support of a host organization, determine where and when to convene, and decide how to address auditions, sound reinforcement, rhythm sections, and funding. The diversity of solutions to these shared challenges reflects the diverse priorities of each founder as well as the unique conditions in which each all-state vocal jazz ensemble was established. However, several elements were common across these stories, including the influence of existing festivals and all-state ensembles, and tensions within the choral education community regarding the value of the vocal jazz idiom relative to traditional choirs, show choirs, and other vocal ensembles. In a few cases, established all-state vocal jazz ensembles were discontinued; these stories further illustrate the challenges such …
Date: August 2022
Creator: Thomas, Tyler
System: The UNT Digital Library