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31 Matching Results
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A Multiplex PCR-coupled Liquid Bead Array for the Simultaneous Detection of Four Biothreat Agents
We have developed a 10-plexed PCR assay coupled to a 12-plexed liquid bead array to rapidly screen environmental samples for B. anthracis, Y. pestis, F. tularensis, and B. melitensis. Highly validated species -specific primer sets were used to simultaneously amplify multiple diagnostic regions unique to each individual pathogen. Resolution of the mix of amplified products was achieved by PCR product hybridization to corresponding probe sequences, attached to unique sets of fluorescent beads. The hybridized beads were processed through a flow cytometer, which detected presence and quantity of each PCR product. The assay was optimized to allow for maximum sensitivity in a multiplexed format. A high- throughput demonstration was performed where 384 simulated environmental samples were spiked with different amounts of B. thuringensis spores and pathogen DNA. The samples were robotically processed to extract DNA and arrayed for multiplexed PCR-liquid bead detection. The assay correctly identified the presence or absence of each pathogen and collected over 3,000 individual data points within a single 8-hour shift for approximately $1.20 per sample in a 10-plexed assay.
Date:
February 4, 2004
Creator:
Wilson, W. J.; Erler, A. M.; Nasarabadi, S. L.; Skowronski, E. W. & McCready, P. M.
Object Type:
Article
System:
The UNT Digital Library
Science and Technology for Bioterrorism Defense
The Lawrence Livermore National Laboratory (LLNL) Chemical & Biological National Security Program (CBNP) provides science, technology, and integrated systems for chemical and biological security. Our approach is to develop and field systems that dramatically improve the nation's capabilities to prevent, prepare for, detect, and respond to terrorist use of chemical or biological weapons.
Date:
May 4, 2004
Creator:
Fitch, J P
Object Type:
Article
System:
The UNT Digital Library
The Clifton Record (Clifton, Tex.), Vol. 109, No. 32, Ed. 1 Friday, June 4, 2004
Weekly newspaper from Clifton, Texas that includes local, state, and national news along with advertising.
Date:
June 4, 2004
Creator:
Smith, W. Leon
Object Type:
Newspaper
System:
The Portal to Texas History
The Clifton Record (Clifton, Tex.), Vol. 109, No. 10, Ed. 1 Wednesday, February 4, 2004
Semi-weekly newspaper from Clifton, Texas that includes local, state, and national news along with advertising.
Date:
February 4, 2004
Creator:
Smith, W. Leon
Object Type:
Newspaper
System:
The Portal to Texas History
Ponderomotive Barrier as a Maxwell Demon
The possibility of efficient ponderomotive current drive in a magnetized plasma was reported recently in [Phys. Rev. Lett. 91, 205004 (2003)]. Precise limitations on the efficiency are now given through a comprehensive analytical and numerical study of single-particle dynamics under the action of a cyclotron-resonant rf drive in various field configurations. Expressions for the particle energy gain and acceleration along the dc magnetic field are obtained. The fundamental correlation between the two effects is described. A second fundamental quantity, namely the ratio of the potential barrier to the energy gain, can be changed by altering the field configuration. The asymmetric ponderomotive current drive effect can be optimized by minimizing the transverse heating.
Date:
May 4, 2004
Creator:
Dodin, I. Y.; Fisch, N. J. & Rax, J. M.
Object Type:
Report
System:
The UNT Digital Library
APDS: The Autonomous Pathogen Detection System
We have developed and tested a fully autonomous pathogen detection system (APDS) capable of continuously monitoring the environment for airborne biological threat agents. The system was developed to provide early warning to civilians in the event of a bioterrorism incident and can be used at high profile events for short-term, intensive monitoring or in major public buildings or transportation nodes for long-term monitoring. The APDS is completely automated, offering continuous aerosol sampling, in-line sample preparation fluidics, multiplexed detection and identification immunoassays, and nucleic-acid based polymerase chain reaction (PCR) amplification and detection. Highly multiplexed antibody-based and duplex nucleic acid-based assays are combined to reduce false positives to a very low level, lower reagent costs, and significantly expand the detection capabilities of this biosensor. This article provides an overview of the current design and operation of the APDS. Certain sub-components of the ADPS are described in detail, including the aerosol collector, the automated sample preparation module that performs multiplexed immunoassays with confirmatory PCR, and the data monitoring and communications system. Data obtained from an APDS that operated continuously for seven days in a major U.S. transportation hub is reported.
Date:
October 4, 2004
Creator:
Hindson, Benjamin; Makarewicz, Anthony; Setlur, Ujwal; Henderer, Bruce; McBride, Mary & Dzenitis, John
Object Type:
Article
System:
The UNT Digital Library
University Press (Beaumont, Tex.), Vol. 80, No. 29, Ed. 1 Wednesday, February 4, 2004
Semiweekly newspaper from Lamar University in Beaumont, Texas that includes local, national, and campus news along with advertising.
Date:
February 4, 2004
Creator:
Gurski, Patrick
Object Type:
Newspaper
System:
The Portal to Texas History
Brady Standard-Herald and Heart O' Texas News (Brady, Tex.), Ed. 1 Friday, June 4, 2004
Semiweekly newspaper from Brady, Texas that includes local, state, and national news along with advertising.
Date:
June 4, 2004
Creator:
Stewart, James E.
Object Type:
Newspaper
System:
The Portal to Texas History
The Dublin Citizen (Dublin, Tex.), Vol. 14, No. 27, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Dublin, Texas that includes local, state, and national news along with advertising.
Date:
March 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Portal to Texas History
The Archer Advocate (Holliday, Tex.), Vol. 2, No. 18, Ed. 1 Wednesday, August 4, 2004
Weekly newspaper from Holliday, Texas that includes local, state, and national news along with advertising.
Date:
August 4, 2004
Creator:
Thomas, John
Object Type:
Newspaper
System:
The Portal to Texas History
Sapulpa Daily Herald (Sapulpa, Okla.), Vol. 89, No. 148, Ed. 1 Thursday, March 4, 2004
Daily newspaper from Sapulpa, Oklahoma that includes local, state, and national news along with advertising.
Date:
March 4, 2004
Creator:
Broaddus, Matthew B.
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Albany News (Albany, Tex.), Vol. 129, No. 23, Ed. 1 Thursday, November 4, 2004
Weekly newspaper from Albany, Texas that includes local, county, and state news along with extensive advertising.
Date:
November 4, 2004
Creator:
Lucas, Melinda L.
Object Type:
Newspaper
System:
The Portal to Texas History
The Tulia Herald (Tulia, Tex.), Vol. 96, No. 45, Ed. 1 Thursday, November 4, 2004
Weekly newspaper from Tulia, Texas that includes local, state and national news along with extensive advertising.
Date:
November 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Portal to Texas History
The Optimist (Abilene, Tex.), Vol. 92, No. 53, Ed. 1, Tuesday, May 4, 2004
Tri-weekly student newspaper from Abilene Christian University in Abilene, Texas that includes local, state and campus news along with advertising.
Date:
May 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Portal to Texas History
The Alvin Advertiser (Alvin, Tex.), Ed. 1 Wednesday, February 4, 2004
Weekly newspaper from Alvin, Texas that includes local, state, and national news along with advertising.
Date:
February 4, 2004
Creator:
Schwind, Jim & Looby, Edward
Object Type:
Newspaper
System:
The Portal to Texas History
Hudspeth County Herald and Dell Valley Review (Dell City, Tex.), Vol. 48, No. 40, Ed. 1 Friday, June 4, 2004
Weekly newspaper from Dell City, Texas that includes local, state, and national news along with advertising.
Date:
June 4, 2004
Creator:
Lynch, Mary Louise
Object Type:
Newspaper
System:
The Portal to Texas History
Port Aransas South Jetty (Port Aransas, Tex.), Vol. 34, No. 10, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Port Aransas, Texas on Mustang Island that includes local, state and national news along with advertising.
Date:
March 4, 2004
Creator:
Judson, Mary
Object Type:
Newspaper
System:
The Portal to Texas History
Stamford American (Stamford, Tex.), Vol. 83, No. 48, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Stamford, Texas that includes local, state, and national news along with advertising.
Date:
March 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Portal to Texas History
Archer County News (Archer City, Tex.), No. 10, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Archer City, Texas that includes local, state, and national news along with advertising.
Date:
March 4, 2004
Creator:
Lewis, Shelley
Object Type:
Newspaper
System:
The Portal to Texas History
The Archer Advocate (Holliday, Tex.), Vol. 1, No. 44, Ed. 1 Wednesday, February 4, 2004
Weekly newspaper from Holliday, Texas that includes local, state, and national news along with advertising.
Date:
February 4, 2004
Creator:
Thomas, John
Object Type:
Newspaper
System:
The Portal to Texas History
Archer County News (Archer City, Tex.), No. 45, Ed. 1 Thursday, November 4, 2004
Weekly newspaper from Archer City, Texas that includes local, state, and national news along with advertising.
Date:
November 4, 2004
Creator:
Lewis, Shelley
Object Type:
Newspaper
System:
The Portal to Texas History
The Albany News (Albany, Tex.), Vol. 128, No. 39, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Albany, Texas that includes local, county, and state news along with extensive advertising.
Date:
March 4, 2004
Creator:
Lucas, Melinda L.
Object Type:
Newspaper
System:
The Portal to Texas History
Seminole Sentinel (Seminole, Tex.), Vol. 95, No. 50, Ed. 1 Sunday, April 4, 2004
Semi-weekly newspaper from Seminole, Texas that includes local, state, and national news along with extensive advertising.
Date:
April 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Portal to Texas History
The Oklahoma Eagle (Tulsa, Okla.), Vol. 83, No. 9, Ed. 1 Thursday, March 4, 2004
Weekly newspaper from Tulsa, Oklahoma that includes local, state, and national news along with advertising.
Date:
March 4, 2004
Creator:
unknown
Object Type:
Newspaper
System:
The Gateway to Oklahoma History