"Down and Out in Paris and London" is typically perceived as non-political. Orwell's first book, it examines his life with the poor in two cities. Although on the surface "Down and Out" seems not to be about politics, Orwell covertly conveys a political message. This is contrary to popular critical opinion. What most critics fail to acknowledge is that Orwell wrote for a middle- and upper-class audience, showing a previously unseen view of the poor. In this he suggests change to the policy makers who are able to bring about improvements for the impoverished. "Down and Out" is often ignored by both critics and readers of Orwell. With an examination of Orwell's politicizing background, and of the way he chooses to present himself and his poor characters in "Down and Out," I argue that the book is both political and characteristic of Orwell's later work.

Organisms synthesize stress proteins in response to a variety of stressors. The 68/70-kDa proteins (synonymous to the 72/73-kDa proteins) have shown to be the most promising stress proteins, and have been proposed as a biomarker of general organismal stress. The 68/70-kDa proteins were used in an antigen/antibody based approach to determine the duration of the stress protein response of Pimephales promelas following an acute exposure to copper sulphate.