Effects of Carbaryl (1-Naphthyl-n-methylcarbamate) on Trichocorixa Reticulata (Hemiptera: Corixidae) and Glyptotendipes Barbipes (Diptera: Chironomidae) (open access)

Effects of Carbaryl (1-Naphthyl-n-methylcarbamate) on Trichocorixa Reticulata (Hemiptera: Corixidae) and Glyptotendipes Barbipes (Diptera: Chironomidae)

My study of the effects of carbaryl in aquatic systems under controlled laboratory conditions emphasized four major objectives: (1) to determine the acute toxicity of carbaryl to the herbivorous adult and immature Trichocorixa reticulata (Guerin)(Hemiptera: Corixidae), and to the omnivorous larvae of Glyptotendipes barbipes (Staeger) (Diptera: Chironomidae) under static bioassay; (2) to adapt a quantitative method of analysis for carbaryl in water and whole insect tissue extract; (3) to measure the accumulation of the insecticide by G. barbipes under static exposure; and (4) to quantify the uptake and loss of carbaryl by G. barbipes under daily-renewed sublethal dosages.
Date: December 1971
Creator: Gash, Stephen L.
System: The UNT Digital Library
Fate of Endothall in Aquatic Environments (open access)

Fate of Endothall in Aquatic Environments

Hazard assessment of pesticides in aquatic environments requires accurate predictions of persistence and compartmentalization. A strategy for developing confidence in predictive fate models, such as the Exposure Analysis Modeling System (EXAMS) and the Simplified Lake and Stream Analysis model (SLSA), is to test the models using carefully chosen chemicals in semi-controlled and field situations. An objective of this approach would be to isolate the variability in a particular fate process and thereby assess the ability of an algorithm to model the process. For example, endothall, a relatively watersoluble aquatic herbicide, has essentially a sole fate process, biotransformation. Endothall was used to test the predictive capabilities of EXAMS and SLSA and to identify sources of variance in those predictions.
Date: December 1984
Creator: Reinert, Kevin H. (Kevin Howard)
System: The UNT Digital Library
Genetics Lecture and Laboratory Syllabus for a Junior-Level Course (open access)

Genetics Lecture and Laboratory Syllabus for a Junior-Level Course

The following is a complete syllabus for a college level genetics course. The syllabus contains lecture outlines and notes for each chapter, along with a list of transparencies needed. The quizzes and exams are prepared and placed at the beginning of the syllabus. The beginning of the course will consist of a lecture to introduce the students to the basics of genetics, followed by many applications of genetics. The process of cell division will be mastered by the students, as well as Mendelian genetics, quantitative genetics, chromosome mapping, and inheritance. The replication, synthesis, and organization of DNA are also discussed within the lectures. The final topics that will be covered using this syllabus are genetics of cancer and immunology and population genetics. These topics are essential for a detailed genetics course. The syllabus is written in great detail, and will require a full semester to be completed. The book used in association with this syllabus is Essentials of Genetics by William S. Klug and Michael R. Cummings.
Date: August 1999
Creator: Harper, Kasey
System: The UNT Digital Library

Factors Affecting MeHg Contamination of Spiders and Insect-Mediated MeHg Flux from Human-Made Ponds

The present study focused on methylmercury (MeHg) in emergent aquatic insects and spiders from human-made ponds. This dissertation addresses two main topics: (1) factors affecting variation in spider MeHg concentrations around human-made ponds and (2) the magnitude of MeHg transported out of human-made ponds by emergent aquatic insects (insect-mediated MeHg flux). Spiders were specifically targeted in this study because they have been proposed as sentinels of MeHg contamination (organism whose tissue concentrations reflect the level of MeHg in the environment). Spider MeHg concentrations were related to spider diet, size, and proximity to waterbody, but affected individual spider taxa differently. In a second study, I found that only "large" spiders within a taxa had tissue concentrations positively related to prey MeHg concentrations. These results indicate that the relationship between spider and prey MeHg could be size-dependent and that "large" spiders within a taxa may better reflect ambient MeHg contamination. Finally, I tested a conceptual model hypothesizing insect-mediated MeHg flux from human-made ponds is controlled by pond permanence and fish presence. In agreement with the conceptual model, insect-mediated MeHg flux from ponds was suppressed by the presence of fish, likely due to fish predation on emergent insect larvae. I found the mean …
Date: December 2023
Creator: Hannappel, Madeline Pratt
System: The UNT Digital Library

Automated Low-cost Instrument for Measuring Total Column Ozone

Access: Use of this item is restricted to the UNT Community
Networks of ground-based and satellite borne instruments to measure ultraviolet (UV) sunlight and total column ozone have greatly contributed to an understanding of increased amounts of UV reaching the surface of the Earth caused by stratospheric ozone depletion. Increased UV radiation has important potential effects on human health, and agricultural and ecological systems. Observations from these networks make it possible to monitor total ozone decreases and to predict ozone recovery trends due to global efforts to curb the use of products releasing chemicals harmful to the ozone layer. Thus, continued and expanded global monitoring of ozone and UV is needed. However, existing automatic stratospheric ozone monitors are complex and expensive instruments. The main objective of this research was the development of a low-cost fully automated total column ozone monitoring instrument which, because of its affordability, will increase the number of instruments available for ground-based observations. The new instrument is based on a high-resolution fiber optic spectrometer, coupled with fiber optics that are precisely aimed by a pan and tilt positioning mechanism and with controlling programs written in commonly available software platforms which run on a personal computer. This project makes use of novel low-cost fiber optic spectrometer technology. A cost …
Date: May 2006
Creator: Nebgen, Gilbert Bernard
System: The UNT Digital Library
Physiological Responses of Myriophyllum spicatum to Time Varying Exposures of Diquat, 2,4-D and Copper (open access)

Physiological Responses of Myriophyllum spicatum to Time Varying Exposures of Diquat, 2,4-D and Copper

The physiological responses of Myriophyllum spicatum to 2,4-D, diquat and copper were quantified using a plant tissue viability assay, and daily measures of dissolved oxygen and pH. Correlations of herbicide tissue residues to physiological response measures were determined and the relationship was used to develop exposure-response models. Diquat and copper had a greater effect on plant tissue viability than was observed for 2,4-D. Diquat produced greater reductions in dissolved oxygen concentrations and pH values than 2,4-D or copper. Copper exposure had the least effect on these parameters. Exposure-response models developed for 2,4-D predicted effective control at plant tissue residues ranging from 4000 to 4700 mg/kg. Aqueous exposure concentrations necessary to produce effective control plant tissue residues ranged from 0.20 to 0.40 mg/L. Exposure-response models developed for diquat predicted effective control at plant tissue residues ranging from 225 to 280 mg/kg. Aqueous exposure concentrations necessary to produce effective control plant tissue residues ranged from 0.113 to 0.169 mg/L. Exposure-response models developed for copper predicted effective control at plant tissue residues ranging from 680 to 790 mg/kg. Aqueous exposure concentrations necessary to produce effective control plant tissue residues ranged from 0.32 to 0.64 mg/L. Model predictions for 2,4-D, diquat and copper were …
Date: May 1988
Creator: Rocchio, Patricia Mary
System: The UNT Digital Library
A Screening of Fungi for Metabolites Inhibitory to the Growth of Bloom-Forming Blue-Green Algae (open access)

A Screening of Fungi for Metabolites Inhibitory to the Growth of Bloom-Forming Blue-Green Algae

Since many approaches to dealing with algal blooms are inefficient, expensive, or harmful, it was concluded that a biologically-synthesized chemical agent, specifically inhibitory to pre-bloom algal cells, might prove helpful in controlling algal blooms. Fungi were chosen as the biological entities to investigate for such a chemical.
Date: December 1970
Creator: Hardcastle, Ronald V.
System: The UNT Digital Library
Effect of Operant Behavior on the Metabolism of 5-Hydroxytryptamine (open access)

Effect of Operant Behavior on the Metabolism of 5-Hydroxytryptamine

The role of operant behavior in the metabolism of brain 5-hydroxytryptamine (5-HT) turnover was investigated. Two and one-half hours following the administration of 150 mg/kg of para-chlorophenylalanine (PCPA), a specific inhibitor of tryptophan hydroxylase, levels of 5-HT were compared in sedentary and performing rats. Whole brain levels of serotonin were reduced in both responding and sedentary animals; however, differences between these groups were not statistically significant. The drug induced decrease in 5-HT levels was accompanied by a significant decrease in session responding. The degree of suppressed responding could be correlated with the level of 5-HT following PCPA, suggesting that the metabolism of serotonin is in part modulated by the rate of responding as maintained by the operant schedule.
Date: August 1979
Creator: Shepard, Paul
System: The UNT Digital Library
Growing Earthworms in Artificial Environments (open access)

Growing Earthworms in Artificial Environments

This study is intended to investigate the artificial environment conditions that would favor the most effective propagation of the African night crawler.
Date: August 1968
Creator: Olaniran, David A.
System: The UNT Digital Library
Linkage Relationships of Greenbug Resistance in Barley, Hordeum Vulgare L. (open access)

Linkage Relationships of Greenbug Resistance in Barley, Hordeum Vulgare L.

The linkage relationship and arm location of the gene for greenbug resistance in the variety Will was determined by using primary trisonomics and tertiary trisomic homozygous translocations. The gene for greenbug resistance was found to be on linkage group 1 by using primary trisonomics. The gene was located on the cetromere segment of the Tl-6a translocation by using a tertiary trismoic homozygous for greenbug resistance. The data further substantiates the feasibility of using trisomics in placing genes on proper linkage groups.
Date: December 1972
Creator: Gardenhire, James H.
System: The UNT Digital Library
A Grassland Evaluation of Eastland County, Texas (open access)

A Grassland Evaluation of Eastland County, Texas

This investigation has had for its purpose the determination of first, all members of the Gramineae (Grass) family found in the county; second, the incidence of each species with the various types of soil; third, the grazing value of each species; fourth, the life span of the parent plants; fifth, the present grassland status; and sixth, the potentialities of developing desirable grassland.
Date: 1951
Creator: Durham, Norman Nevill
System: The UNT Digital Library
Biochemical Genetics of Certain Species of the Blackbird Family Icteridae (open access)

Biochemical Genetics of Certain Species of the Blackbird Family Icteridae

Starch gel electrophoresis was used to compare 14 proteins encoded by 15 loci for seven species of the family Icteridae. A close genetic relationship among these species was classified into three groups. The Agelaiine group contained Agelaius phoeniceus, Sturnella magna, and S. neglecta. The Quiscaline group contained Euphagus cyanocephalus, Cassidix mexicanus, and Quiscalus quiscula. Molothrus ater, the most divergent, was placed in a separate group. Divergence times for the seven species were compared to the literature. Heterozygosity of the seven populations of the two species of Sturnella were compared to determine factors influencing their divergence. Two factors proposed were heterosis in S. neglecta and possible hybridization between S. neglect and S. magna.
Date: December 1974
Creator: Smith, Jackson Kelly
System: The UNT Digital Library
Evolutionary Genetics of Three Semispecies of Wood Rats--Neotoma Albigula, Neotoma Micropus, and Neotoma Floridana (open access)

Evolutionary Genetics of Three Semispecies of Wood Rats--Neotoma Albigula, Neotoma Micropus, and Neotoma Floridana

Electrophoretic variation in 18 proteins encoded by 20 autosomal loci was used to compare the genetic relationships of 19 natural populations representing three species of the subgenus Neotoma. Of the 20 loci examined nine were monomorphic and fixed for the same allele in all populations. No more than seven loci were polymorphic within a single population. Genetic variability was expressed as the proportion of loci heterozygous in the average individual of a population. Heterozygosity in the three species of Neotoma studied averaged 0.078, a value within the range reported for other rodents. Although the levels of heterozygosity seen in Neotoma could not readily be explained, the variation may be attributed to ecological factors. The three species of Neotoma were compared on the basis of genetic similarity and found to form a close taxonomic unit, probably semispecies. Divergence times were obtained for the three species and found to compare well with divergence times obtained from fossil data. In general, the three species have diverged within the last 112,000 years during the Wisconsin glacial period.
Date: December 1975
Creator: Nejtek, Michael E.
System: The UNT Digital Library
The Effect of Carbon Monoxide on the Growth of an Aquatic Streptomycete (open access)

The Effect of Carbon Monoxide on the Growth of an Aquatic Streptomycete

The major purpose of this investigation was to determine the effect of carbon monoxide on the development of various morphological phases of a single isolate of the aquatic streptomycetes and to elucidate the physiological differences between terminal respiration in the primary and secondary stages. A secondary purpose was to repeat the work of Michael Lee Higgins involving the effect of anaerobiosis on the growth of the organism.
Date: August 1966
Creator: Francisco, Donald Edward
System: The UNT Digital Library
A Grassland Evaluation of the W. A. McKamy Ranch, Denton and Wise Counties, Texas (open access)

A Grassland Evaluation of the W. A. McKamy Ranch, Denton and Wise Counties, Texas

This problem consists of classifying the four major pastures of the McKamy ranch into McConnell's four categories, by means of examinations based on these qualities and characteristics.
Date: 1950
Creator: Meacham, William Ross
System: The UNT Digital Library
Genetic Variation in a Population of the Plains Woodrat Neotoma micropus (open access)

Genetic Variation in a Population of the Plains Woodrat Neotoma micropus

Neotoma micropus from Jack County, Texas, were studied over a 9-month period. Loci from blood and saliva were used to determine genetic variation within the population. Deviations from Hardy-Weinberg equilibrium were found at one locus. The average temporal F over all seven loci was 0.040. Genetic structuring was subtle, fluctuated on a seasonal basis, and was due to differential migration or predation on genotypes. Heterozygotes tended to move more than homozygotes, and a greater proportion of heterozygotes were lost from the population during each season. Genetic variation was maintained in the population by immigrant individuals. This differential in dispersal of genotypes fits current models of reorganization within the genome of populations.
Date: August 1988
Creator: Stewart, John E. B. (John Edward Bakos)
System: The UNT Digital Library
Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio). (open access)

Influence of parental swimming stamina on the cardiac and metabolic performance of larval zebrafish (Danio rerio).

Superior swimming stamina in adult fish is presumably passed on to their offspring, but the ontogeny of the appearance of superior stamina and the requisite enhanced cardio-respiratory support for locomotion in larval fishes has not been determined. Is the expression of the suite of parental traits enabling superior swimming stamina in their offspring dependent upon their achieving juvenile/adult morphology, or does it appear earlier in their larvae? To answer this, adults were classified into three groups based on swimming stamina, followed by measurement of length, mass, and width. Larval offspring from the two parental groups -high stamina larvae (HSL) and low stamina larvae (LSL)- were reared at 27°C in aerated water (21% O2). Routine and active heart rate, routine and active mass specific oxygen consumption were recorded through 21dpf, and cost of transport (COT) and factorial aerobic scope were derived from oxygen consumption measurements. Routine heart rate at 2dpf of LSL was 164 ± 1 b·min-1, compared to only 125 ± 2 b·min-1 for HSL. Routine heart rate subsequently peaked at 203 ± 1 b·min-1 at 5dpf in the HSL group, compared to 207 ± 1 b·min-1, at 4dpf in the LSP larvae. Active heart rate at 5 dpf of …
Date: May 2007
Creator: Gore, Matthew R.
System: The UNT Digital Library
Responses of Fishes to a Low pH Environment (open access)

Responses of Fishes to a Low pH Environment

Data were collected from natural and introduced fishes present in Ferndale Lake, a small (120 ha) sport fishing reservoir in Camp County, east Texas. Levels of pH measured in the lake during the study period ranged from 3.5 to 5.3. Monthly field surveys and experimental manipulations were designed to evaluate quantitatively the signs of stress at various biological levels. Lethal limits to low pH were quantified for largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) to be pH 3.8 and 4.0,respectively. Mean blood pH (+ 1 SD) of 59 bluegill was 7.41 (j 0.16), with no significant difference (P-0.05) among groups from Ferndale Lake and Moss Lake (Cooke Co., Texas) under experimental conditions, even when severe stress was externally apparent. In a dual-trough horizontal pH gradient, bluegill behavioral avoidance was observed at pH levels below 7.0. Individual testing of 40 bluegill in pH gradient of 5.2 to 7.6 resulted in median occupation of pH 7.1,with an interquartile range of pH 6.9 to 7.3. Decreased community structure and population "well being" compared to early studies cannot be attributed entirely to recent acidic condition. Separating potential stress due to lake conditions from that due to heavy biotic predation by sport fishing in …
Date: August 1981
Creator: Prete, Philip J. (Philip John)
System: The UNT Digital Library
Responses of Selected Texas Fishes to Abiotic Factors, and an Evaluation of the Mechanisms Controlling Thermal Tolerance of the Sheepshead Minnow (open access)

Responses of Selected Texas Fishes to Abiotic Factors, and an Evaluation of the Mechanisms Controlling Thermal Tolerance of the Sheepshead Minnow

Low oxygen tolerances of ten fishes were estimated using an original nitrogen cascade design, and reciprocally transformed to express responses as ventilated volume necessary to satisfy minimal oxygen demand (L·mg O2^-1). Values ranged from 0.52 to 5.64 L·mg^-1 and were partitioned into three statistically distinct groups. Eight stream fishes showed moderately high tolerances reflecting metabolic adaptations associated with stream intermittency. Juvenile longear sunfish and two mollies comprised the second group. High tolerance of hypoxia may allow juvenile sunfish to avoid predation, and mollies to survive harsh environmental oxygen regimens. The sheepshead minnow was the most tolerant species of low oxygen, of those examined, explaining its presence in severely hypoxic environments.
Date: May 1994
Creator: Bennett, Wayne A. (Wayne Arden)
System: The UNT Digital Library
The Inheritance of Serum Alkaline Phosphatase in the Pigeon (Columba livia) (open access)

The Inheritance of Serum Alkaline Phosphatase in the Pigeon (Columba livia)

The purpose of this work was to determine the manner of inheritance of serum alkaline phosphatase in the racing pigeon, (Columba livia). The evidence indicates that the electrophoretic patterns of serum alkaline phosphatase in the pigeon are inherited as codominant genes.
Date: August 1968
Creator: Manley, James H., Jr.
System: The UNT Digital Library
Nitrogen Accretion on a Lacustrine Plain (open access)

Nitrogen Accretion on a Lacustrine Plain

The purpose of the investigation was to locate the plant population which had the greatest impact on soil nitrogen in a successional sequence from newly deposited alluvia to a mature streamside forest, and to evaluate the pioneer populations in terms of their annual nitrogen contribution.
Date: May 1972
Creator: Davis, Karla S.
System: The UNT Digital Library
Trophic Structure and Energy Flow in a Texas Pond (open access)

Trophic Structure and Energy Flow in a Texas Pond

Annual energy flow and mean annual biocontent of eighteen compartments were determined for a 0.94 ha north central Texas pond ecosystem. Annual primary production was 7,780 kcal m^-2 yr^-2, and community production-to-respiration ratio was 1.49. One-third of annual primary production accumulated on the substrate as silt and sedimentation. Community production, production-respiration ratio, and biocontents of all compartments except aquatic insects were large in summer, small in winter. Biocontents of four trophic levels in the pond were all of the same order of magnitude, approximately 50 kcal m^-2. Suspended and benthic organic material forprimary consumers and terrestrial insects for tertiary consumers were substantial allochthanous energy imports into the pond system.
Date: August 1978
Creator: Childress, William M.
System: The UNT Digital Library
Effector Response of the Aspartate Transcarbamoylase From Wild Type  Pseudomonas Putida and a Mutant with 11 Amino Acids Deleted at the N-terminus of PyrB. (open access)

Effector Response of the Aspartate Transcarbamoylase From Wild Type Pseudomonas Putida and a Mutant with 11 Amino Acids Deleted at the N-terminus of PyrB.

Like its enteric counterpart, aspartate transcarbamoylase (ATCase) from Pseudomonas putida is a dodecamer of two different polypeptides. Unlike the enterics, the Pseudomonas ATCase lacks regulatory polypeptides but employs instead inactive dihydroorotases for an active dodecamer. Previous work showed that PyrB contains not only the active site but also the effector binding sites for ATP, UTP and CTP at its N-terminus. In this work, 11 amino acids were deleted from the N-terminus of PyrB and the ATCase with the truncated protein was expressed in E. coli pyrB- and purified. The wild type enzyme was similarly treated. Velocity-substrate plots without effectors gave Michaelis-Menten kinetics in all cases. Deleting 11 amino acids did not affect dodecameric assembly but altered effector responses. When carbamoylphosphate was varied, the mutant enzyme was inhibited by UTP while the wild type enzyme was activated 2-fold. When the aspartate was varied, CTP had no effect on the mutant enzyme but strongly inhibited the wild type enzyme.
Date: May 2002
Creator: Asfour, Hani
System: The UNT Digital Library
Optimization of In Vitro Mammalian Blastocyst Development: Assessment of Culture Conditions, Ovarian Stimulation and Experimental Micro-Manipulation (open access)

Optimization of In Vitro Mammalian Blastocyst Development: Assessment of Culture Conditions, Ovarian Stimulation and Experimental Micro-Manipulation

Factors currently at the forefront of human in vitro fertilization (IVF) that collectively influence treatment success in the form of blastocysts development were investigated during early mammalian embryology with concentration on infertile patients presenting with diminished ovarian reserve or preliminary ovarian failure. A novel experimental technique, Graft Transplant-Embryonic Stem Cells (GT-ESC) was introduced in the mouse model, as the first inclusive approach for embryo selection in IVF treatments resulting in successful graft integration of sibling cells, stage-dependent (day 4) blastocysts. E-Cadherin-catenin bonds play an integral role in trophectoderm cell viability and calcium removal, inducing disruption of cell-to-cell bonds at the blastocyst stage was detrimental to continued blastocyst development. One of the leading methods for embryo selection for uterine transfer in human IVF is application of pre-implantation genetic screening (PGS) methods such as next generation sequencing (NGS). Female patients <35 y do not benefit from this treatment when outcome is measured by presence of fetal heart beats at 10 weeks of gestation. Patients 35-37 y benefit from PGS with no significant difference of outcome based on form of PGS method utilized. Therefore, small nucleotide polymorphism array (snp-array) or targeted-NGS should be selected for this age range to lessen the financial burden …
Date: May 2018
Creator: Sadruddin, Sheela
System: The UNT Digital Library