Spatial ability is the skill associated with mental relations among objects, the process of maintaining the physical aspects of an object after mentally rotating it in space. Many studies report a strong association of spatial ability with success in various areas of health care, especially surgery, radiology and dentistry. To date, similar investigations in professional nursing could not be located. Registered nurses, employed in an acute care multi-hospital setting, were surveyed using the Shipley-2Block Pattern Test, the Group Embedded Figures Test, and a newly created test of general nursing knowledge. The sample size of 123 nurses was composed of 31 male nurses and 92 female nurses. Data was collected between May and August of 2013 and analyzed using R, version 2.15.2. The present study did not demonstrate a statistically significant effect for gender differences on two measures of spatial ability. However, Cohen’s d effect sizes for mean gender differences in the present study are consistent with prior studies. This may suggest the nursing profession is comparable with other professions where males perform higher than females on spatial ability. The present study should be considered an initial step toward evaluating the relevance of spatial ability in the performance of nursing care.