The study herein described involved the detection of pyridine N-oxide reductase activity in cell-free extracts of E. coli 9723, the determination of co-factors necessary for the enzymatic process, a study of the optimum conditions for enzyme catalysis, and a general characterization of the enzyme.

The research described herein is concerned with the synthesis of certain organic compounds which have the amino-oxy grouping and are related in structure to the naturally occurring amines, putrescine, spermidine and spermine.

This study is the result of tests conducted to determine the adaptability of the human eye to darkness.

This study reported herein involves the synthesis and determination of certain biological activities of 4,5-dihydroxy-2-pyridinealanine and the synthesis of 3-pyridine-N-methylalanine.

A study of the ascorbic acid metabolism of a group of fifty college women in the North Texas State Teachers College between the months of April and July, 1943.

The levels of total nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), and their redox states were determined as the function of growth in S. cerevisiae. Cells growing in a medium containing 0.8% glucose exhibit two phases of exponential growth, utilizing glucose and ethanol, respectively. The NAD pool is 50% reduced during both stages of growth while the NADP pool is 67% reduced in glucose growth and 48% reduced in ethanol growth. The NAD/NADP ratio is constant during growth on glucose and a two-fold increase in the NAD/NADP ratio occurs upon exhaustion of glucose. The increased ratio is maintained during growth on ethanol. This alteration in the regulation of the relative levels of NAD and NADP may be due to a change in the regulation of NAD kinase and/or NADP phosphatase activities. These changes may be related to the redox state of the NADP pool.

An experiment was designed to study in the same animal any preferential absorption of a free fatty acid in the presence of a triglyceride of the same fatty acid. Rats were administered a mixture of free fatty acid and its triglyceride labeled with carbon-13 and carbon-14 respectively. Each isotope in the fed lipid and in the lipid recovered from the gastrointestinal tract was measured. The isotope effect, if any, was studied by administering a mixture of palmitic acid-1-C13 and palmitic acid-1-C14.

The cytoplasmic enzyme, NAD⁺ kinase (ATP: NAD⁺ 2-phosphotransferase, [E.C. 2.7.1.23}) has been characterized and purified from yeast. A continuous fluorescence assay was developed. A purification procedure was developed utilizing NAD⁺-Agarose affinity column chromatography.

The increasingly complex world of illicit chemistry has created a need for rapid, selective means of determining the threat posed by new drugs as they are encountered by law enforcement personnel. To streamline this process, the entirety of the problem, from the production of illicit drugs all the way to the final analysis have been investigated. A series of N-alkylated phenethylamine analogues were synthesized in a shotgun method and subjected to direct-infusion analysis. A range of products were detected without the need for time-consuming purification steps, which was extended to novel pharmacological and receptor-binding assays where mass spectrometry is used as a detector. This direct-infusion technique was also applied to studies of methamphetamine and fentanyl production to preemptively determine improvements to common reaction conditions and explore the origins of common impurities. The ability to utilize these rapid techniques directly from the fume hood has also been critically reviewed to highlight gaps in current research and opportunities for improvement. When combined, these studies seek to provide a means for rapid, simplified analysis of illicit drugs to improve the quality of data and dramatically increase throughput.

Molecular dynamics simulations and QM/MM calculations can provide insights into the structure and function of enzymes as well as changes due to mutations of the protein sequence.

Poor medical adherence attributed to patient compliance has impacted the medical community, at times, in a deleterious fashion. To combat this, the medical community has attempted to provide therapeutics in the form of absorption enhancing techniques. To improve the absorption rate techniques such as drug encapsulation using proteins, liposomes, or nanotransfersomes have been developed using mass spectrometry. These techniques, have aided in the enhanced absorption of analytes with low bioavailability, including curcumin, simvastatin, and lysozyme. Specifically, mass spectrometry allows for the development and monitoring of nanotransfersome encapsulated analytes and the permeation across the dermal membrane. This transdermal delivery would eliminate the problems encountered during first pass metabolism, while allowing for higher concentrations of analyte to be maintained in the blood serum. This can be coupled to a thermosensitive gelatin that provides for a dose control mechanism to be accomplished, allowing multiple doses to be delivered using one transdermal patch system. The novel delivery system developed using mass spectrometry, allows the analyte to be delivered into the circulatory system at a controlled dosage, via transdermal absorption. This system will aid in eliminating problems associated with patient compliance, as the patient is no longer reliant on memory to self-dose. Further, this system …

A series of acene-fused porphyrins and 1,10-phenanthroline-fused porphyrins were synthesized and characterized via NMR spectroscopy and mass spectrometry. The acene-fused porphyrins exhibit unique optoelectronic properties, most notably they exhibit highly red-shifted absorption bands. The 1,10-phenanthroline-fused porphyrins are of interest for their ability to bond to as variety of metals to form chelation complexes.