Due to the wide spread occurrence of medroxyprogesterone acetate (MPA), a pharmaceutical compound, in wastewater effluent and surface waters, the objectives of this work were to determine the tissue specific uptake and bioconcentration factor (BCF) for MPA in common carp. BCFs were experimentally determined for MPA in fish using a 14-day laboratory test whereby carp where exposed to 100 μg/L of MPA for a 7-day period followed by a depuration phase in which fish were maintained in dechlorinated tap water for an additional 7 days. MPA concentrations in muscle, brain, liver and plasma were determined by liquid chromatography/mass spectrometry (LC/MS). The results from the experiment indicate that MPA can accumulate in fish, however, MPA is not considered to be bioaccumulative based on regulatory standards (BCF ≥ 1000). Although MPA has a low BCF value in common carp, this compound may cause reproductive effects in fish at environmentally relevant concentrations.