Delayed hypersensitivity can be conferred passively to normal animals. There exists a period when whole peritoneal exudate cells will passively confer delayed sensitivity, but a sonic extract from them will not; however, after a few more days, both whole cells and sonic extracts could transfer sensitivity. This investigation was undertaken to study the differences in cells collected at two different time intervals after initial sensitization of guinea pigs with 1-fluoro-2,4-dinitrobenzene.

The purpose of this study is to determine the presence or absence of a typical Krebs cycle metabolism in the primary mycelia of an aquatic streptomycete.

The major purpose of this investigation was to determine the effect of carbon monoxide on the development of various morphological phases of a single isolate of the aquatic streptomycetes and to elucidate the physiological differences between terminal respiration in the primary and secondary stages. A secondary purpose was to repeat the work of Michael Lee Higgins involving the effect of anaerobiosis on the growth of the organism.

Having considered the data collected thus far concerning immediate and delayed hypersensitivity reactions in the mouse, the experimentation following is devised essentially to follow the pattern of Crowle in the production and testing for immunoresponse. Instead of using the normal solvent, that is, acetone, this work is also intended to partially evaluated the use of dimethyl sulfoxide as a solvent for the contact allergin, dinitroflurobenzene.

The induction of phenylketonuria in mice through the use of excess dietary phenylalanine is an area in which limited research has been done. This study intends to pursue further work in this area, more specifically, to study the effects of excess dietary phenylalanine and the phenylalanine analogue A.P.B.A. (2-amino-3-phenyl butanoic acid) (7) on brain serotonin and brain norepinephrine. In addition, the effects of these two compounds on the incidence of audiogenic seizures will be explored.

The purpose of the current study is to determine something of the nature of the heat resistance in Azotobacter, if in fact this is found to exist. An attempt is made to determine the specific physiological state associated with heat resistance as well as to resolve this resistance quantitatively.

The purpose of this study is to determine if a strain of Azotobacter chroococcum isolated from the soil in northern Louisiana produces cysts which are as resistant to deleterious agents as those produced by previously reported strains os Azotobacter.

Exactly where in the hypothalamus is the adrenal-pituitary response to X-irradiation "triggered" or initiated? Moreover, does ionizing radiation act directly on specific centers in the brain or does it act indirectly via the production of some humoral agents? Finally, what role does the hypothalamus play in the radiation-syndrome? The purpose of the present study was to attempt to answer these questions by determining the effects of two stressor agents, X-irradiation and electrical stimulation applied either singly or together, on the activity of the adrenal-pituitary axis. The parameters measured were changes in plasma corticosterone, in circulating eosinopihils, and in adrenal gland weight.

The aim of this study is to determine the changes in respiration in tissues from specific brain areas X-irradiated in vitro using a direct monometric procedure also to determine the differences in radiosensitivity of specific areas in the brain as reflected by changes in oxygen uptake and to shed some light on the nature of the biochemical lesions in nerve tissues brought about by ionizing radiation.

The purpose of this study was twofold: 1) to present experimental data on changes in permeability of fibers during irradiation using Na22 as the tracer isotope, and 2) to attempt to correlate these findings with the electrical changes observed.

In this study organisms that can be subcultured from lake water, using a prescribed procedure, limit, to an extent, the population, or portions of the population, that can be monitored. In essence, what is taking place is that a set of conditions is set forth and a study is made of the bacteria that will grow under these prescribed conditions.

It was the purpose of the present investigation to determine if sensitivity to other antibiotics can likewise be affected by subjecting S. aureus to heparin contact. It is of special interest in this problem to determine whether heparin in some manner affects the combining process of penicillin with the cells of several strains of S. aureus.

The purpose of this investigation was an attempt to clarify the relatives taxonomic position of and to formulate some of the basic cyclic morphological and physiological processes occurring in an aquatic actinomycete possessing streptomycetal characteristics.

It is the purpose of this investigation to determine the variation of glyoxalase activity in liver of normal mice and in the liver of mice bearing a lymphosarcoma and to compare the glyoxalase activity of the lymphosarcoma with values previously reported in the literature for other tumor types. Further, if there is indeed a variation in liver glyoxalase activity between normal and tumor-bearing mice, it will be compared to the variation in the activity of two other enzymes present in liver tissue in relatively high concentration.

The purpose of the present study was sixfold: 1. To study the effects of x-irradiation on spinal cord activity. 2. To study the effects of CNS drugs on spinal cord function as reflected by changes in the ventral root potentials. 3. To ascertain whether one can alter the observed spinal response to ionizing radiation by applying CNS drugs prior to, during, and following x-irradiation of a given spinal cord segment. 4. To shed some light on the role of higher brain centers on spinal reflexes. 5. To shed some light on the loci of radiation insult to the spinal cord. 6. To establish evidence for a possible drug-irradiation interaction in mammals.

This research is concerned specifically with the growth of two selected algae found surviving in chlorinated water in outdoor swimming pools. Unialgal cultures were used to determine the free chlorine residuals that these algae were able to tolerate.

This thesis was written to study photoreactivation in different physiological conditions of the vegetative cell as well as the photoreactivation of the two morphological states of the Azotobacter cell: the vegetative cell and the cyst.

Many experiments have been performed with the Warburg apparatus, or variations of this manometric technique, since Warburg's experiments (52, 53) where the effects of cyanides upon dark reactions and of urethanes upon light reactions of photosynthesis were demonstrated. The same basic techniques were utilized in this research in attempting to determine the effects of some coordination complexes upon the apparent photosynthetic rate of Chlorella pyrenoidosa. A second goal of the present paper was to investigate the potential of the Warburg apparatus as a tool for screening algicidal compounds.

The purpose of this study, therefore, was threefold in nature: (1) to determine the effects of x-irradiation on the influx and efflux of potassium in compound nerve fibers (2) to attempt to relate the radiation-induced changes in electrical activity with potassium flux and (3) to use the information obtained to gain insight into the possible cellular site (s) of radiation insult to compound nerves.

The purpose of this investigation is to determine if differences exist between four inbred strains of mice in susceptibility to the drug thalidomide.

The current work was undertaken to test the ability of avidin to affect the course of leukemia when administered intraperitoneally.

It was the purpose of this investigation to present laboratory data concerning the assimilation of inorganic nitrogen by the aquatic actinomycetes. The strains of aquatic actinomycetes under consideration represented a cross section of those currently under culture at North Texas State University.

The purpose of the present study was twofold: (1) to determine if X-irradiation can be considered a direct stress agent, and if so, to what extent it differs from other stressors; and (2) to further elucidate the role of the adrenal cortex in the radiation syndrome by determining the more immediate responses of this system to X-irradiation.