'Study the past--what is past is prologue'. These words appear as the motto on a pair of statues at the National Archives Building in Washington DC. They are also the opening sentence in the preface of a document written in August of 1956 entitled 'A Summary of Accidents and Incidents Involving Radiation in Atomic Energy Activities--June 1945 thru December 1955'. This document, one of several written by D.F. Hayes of the Safety and Fire Protection Branch, Division of Organization and Personnel, U.S. Atomic Energy Commission in Washington DC, and many others are often forgotten even though they contain valuable glovebox fire protection lessons for us today.

Gene probe hybridization was used to determine distribution and expression of co-metabolic genes at a contaminated site as it underwent in situ methanotrophic bioremediation of trichloroethylene (TCE). The bioremediation strategies tested included a series of air, air:methane, and air:methane:nutrient pulses of the test plot using horizontal injection wells. During the test period, the levels of TCE reduced drastically in almost all test samples. Sediment core samples (n = 367) taken from 0 m (surface)-43 m depth were probed for gene coding for methanotrophic soluble methane monooxygenase (sMMO) and heterotrophic toluene dioxygenase (TOD), which are known to co-metabolize TCE. The same sediment samples were also probed for genes coding for methanol dehydrogenase (MDH) (catalyzing the oxidation of methanol to formaldehyde) to assess specifically changes in methylotrophic bacterial populations in the site. Gene hybridization results showed that the frequency of detection of sMMO genes were stimulated approximately 250% following 1% methane:air (v/v) injection. Subsequent injection of 4% methane:air (v/v) resulted in an 85% decline probably due to nutrient limitations, since addition of nutrients (gaseous nitrogen and phosphorus) thereafter caused an increase in the frequency of detection of sMMO genes. Detection of TOD genes declined during the process, and eventually they were non-detectable …

This paper is a product for the Department of Energy?s (DOE) Office of Science (OS) reporting on the feasibility of using HPSS into the Extreme Scale era of storage (2018 -2022). The initial sections provide a summary of the systems environment and expected archival storage requirements extracted from other Extreme Scale workshopreports conducted since 2007 by various applications and programs within the DOE OS. These high level requirements aid in identifying long-term data storage system features that support Extreme Scale science. Participants also separately forecasted data growth in established long-term data storage systems through 2018 - 2022 to get a picture of the amount of data that systems will need to manage. The report concludes that HPSS is well positioned to meet the requirements projected for the Extreme Scale era and provides recommendations from the HPSS Collaboration to the DOE Office of Science for ensuring that HPSS can meet these extreme scale storage requirements of 2018 - 2022.

Cometabolic bioremediation is probably the most under appreciated bioremediation strategy currently available. Cometabolism strategies stimulate only indigenous microbes with the ability to degrade the contaminant and cosubstrate e.g. methane, propane, toluene and others. This highly targeted stimulation insures that only those microbes that can degrade the contaminant are targeted, thus reducing amendment costs, well and formation plugging, etc. Cometabolic bioremediation has been used on some of the most recalcitrant contaminants, e.g. PCE, TCE, MTBE, TNT, dioxane, atrazine, etc. Methanotrophs have been demonstrated to produce methane monooxygense, an oxidase that can degrade over 300 compounds. Cometabolic bioremediation also has the advantage of being able to degrade contaminants to trace concentrations, since the biodegrader is not dependent on the contaminant for carbon or energy. Increasingly we are finding that in order to protect human health and the environment that we must remediate to lower and lower concentrations, especially for compounds like endocrine disrupters, thus cometabolism may be the best and maybe the only possibility that we have to bioremediate some contaminants.

This is a report on the comebiotic bioremediation which is the most under-appreciated strategy currently available.

The Hanford Site environmental report is prepared annually for the U.S. Department of Energy (DOE) in accordance with regulatory requirements. The report provides an overview of activities at the Hanford Site; demonstrates the status of the site’s compliance with applicable federal, state, and local environmental laws and regulations, executive orders, and DOE policies and directives; and summarizes environmental data that characterize Hanford Site environmental management performance. The report also highlights significant environmental and public protection programs and efforts. Some historical and early 2009 information is included where appropriate.