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Three-dimensional lithographically-defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression
Here we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following one to three days. The protocol can easily be adapted to study a variety of tissues and aspects of normal and neoplastic development.
Date:
February 13, 2008
Creator:
Nelson, Celeste M.; Inman, Jamie L. & Bissell, Mina J.
Object Type:
Article
System:
The UNT Digital Library
Cherokeean Herald (Rusk, Tex.), Vol. 158, No. 51, Ed. 1 Wednesday, February 13, 2008
Weekly newspaper from Rusk, Texas that includes local, state and national news along with extensive advertising.
Date:
February 13, 2008
Creator:
Whitehead, Marie
Object Type:
Newspaper
System:
The Portal to Texas History
Perry Daily Journal (Perry, Okla.), Vol. 116, No. 31, Ed. 1 Wednesday, February 13, 2008
Daily newspaper from Perry, Oklahoma that includes local, state, and national news along with advertising.
Date:
February 13, 2008
Creator:
Brown, Gloria
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
The Altus Times (Altus, Okla.), Vol. 109, No. 219, Ed. 1 Wednesday, February 13, 2008
Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.
Date:
February 13, 2008
Creator:
Bush, Michael
Object Type:
Newspaper
System:
The Gateway to Oklahoma History
Burleson Star (Burleson, Tex.), Vol. 43, No. 40, Ed. 1 Wednesday, February 13, 2008
Semiweekly newspaper from Burleson, Texas that includes local, state, and national news along with advertising.
Date:
February 13, 2008
Creator:
Rayburn, Michael
Object Type:
Newspaper
System:
The Portal to Texas History
The Llano News (Llano, Tex.), Vol. 120, No. 20, Ed. 1 Wednesday, February 13, 2008
Weekly newspaper from Llano, Texas that includes local, state, and national news along with advertising.
Date:
February 13, 2008
Creator:
Stephenson, Jimmy
Object Type:
Newspaper
System:
The Portal to Texas History