Background: Microbial life dominates the earth, but many species are difficult or even impossible to study under laboratory conditions. Sequencing DNA directly from the environment, a technique commonly referred to as metagenomics, is an important tool for cataloging microbial life. This culture-independent approach involves collecting samples that include microbes in them, extracting DNA from the samples, and sequencing the DNA. A sample may contain many different microorganisms, macroorganisms, and even free-floating environmental DNA. A fundamental challenge in metagenomics has been estimating the abundance of organisms in a sample based on the frequency with which the organism's DNA was observed in reads generated via DNA sequencing. Methodology/Principal Findings: We created mixtures of ten microbial species for which genome sequences are known. Each mixture contained an equal number of cells of each species. We then extracted DNA from the mixtures, sequenced the DNA, and measured the frequency with which genomic regions from each organism was observed in the sequenced DNA. We found that the observed frequency of reads mapping to each organism did not reflect the equal numbers of cells that were known to be included in each mixture. The relative organism abundances varied significantly depending on the DNA extraction and sequencing …

The National Museum of the Pacific War presents an interview with Walter Lamb. Lamb participated in the Civilian Conservation Corps in the late 1930s. He joined the Navy around 1940. In April of 1941 he traveled to California for basic training and Ordnance School. He joined Utility Squadron 2 (VJ-2) and traveled aboard the USS Ramapo (AO-12) to the Philippines. On 6 December 1941, they stopped over at Pearl Harbor. Lamb was on the island when the Japanese attacked the next day. He remained on Ford Island until December of 1942, assisting with debris clean up. He served as Ordnanceman aboard an unnamed, in the South Pacific, transporting airplanes and supplies.

Interview with Maria Silva Ayala Garza, descendant of Mexicans born in Mexican territory that is now in Texas, from Kerrville, Texas. Mrs. Garza talks about her family's history in the area before it became incorporated into Texas. She also talks about growing up in Kerrville, as well as local people and places. Included in the interview are pictures of Mrs. Garza and her family, on pages 28-32.

Student newspaper of the University of Oklahoma in Norman, Oklahoma that includes national, local, and campus news along with advertising.

The National Museum of the Pacific War presents an interview with Walter Lamb. Lamb participated in the Civilian Conservation Corps in the late 1930s. He joined the Navy around 1940. In April of 1941 he traveled to California for basic training and Ordnance School. He joined Utility Squadron 2 (VJ-2) and traveled aboard the USS Ramapo (AO-12) to the Philippines. On 6 December 1941, they stopped over at Pearl Harbor. Lamb was on the island when the Japanese attacked the next day. He remained on Ford Island until December of 1942, assisting with debris clean up. He served as Ordnanceman aboard an unnamed, in the South Pacific, transporting airplanes and supplies.

The objective of this project was to provide wind resource inputs to the Eastern Wind Integration and Transmission Study.

Daily newspaper from Altus, Oklahoma that includes local, state, and national news along with advertising.

Corrective Action Unit 367 is located in Area 10 of the Nevada Test Site, which is approximately 65 miles northwest of Las Vegas, Nevada. Corrective Action Unit 367 comprises the four corrective action sites (CASs) listed below: • 10-45-01, U-10h Crater (Sedan) • 10-45-02, Ess Crater Site • 10-09-03, Mud Pit • 10-45-03, Uncle Crater Site The CASs in CAU 367 are being investigated because hazardous and/or radioactive contaminants may be present in concentrations that exceed risk-based corrective action (RBCA) levels. Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend CAAs for the CASs. Additional information will be generated by conducting a CAI before evaluating and selecting CAAs. The scope of the corrective action investigation for CAU 367 includes the following activities: • Move surface debris and/or materials, as needed, to facilitate sampling. • Conduct radiological surveys. • Collect and submit environmental samples for laboratory analysis to determine the area where TED at the site exceeds FALs (i.e., corrective action boundary). • Evaluate TED to potential receptors in areas along Mercury Highway that have been impacted by a release of radionuclides from the Sedan test. • Collect and submit environmental samples for laboratory …