This chapter will address some of the many issues associated with the identification of signatures based on genomic DNA/RNA, which can be used to identify and characterize pathogens for biodefense and microbial forensic goals. For the purposes of this chapter, we define a signature as one or more strings of contiguous genomic DNA or RNA bases that are sufficient to identify a pathogenic target of interest at the desired resolution and which could be instantiated with particular detection chemistry on a particular platform. The target may be a whole organism, an individual functional mechanism (e.g., a toxin gene), or simply a nucleic acid indicative of the organism. The desired resolution will vary with each program's goals but could easily range from family to genus to species to strain to isolate. The resolution may not be taxonomically based but rather pan-mechanistic in nature: detecting virulence or antibiotic-resistance genes shared by multiple microbes. Entire industries exist around different detection chemistries and instrument platforms for identification of pathogens, and we will only briefly mention a few of the techniques that we have used at Lawrence Livermore National Laboratory (LLNL) to support our biosecurity-related work since 2000. Most nucleic acid based detection chemistries involve …

Prokaryotic life on earth is manifested by its diversity and omnipresence. These microbes serve as natural sources of a large variety of compounds with the potential to serve the ever growing, medicinal, chemical and transportation needs of the human population. However, commercially viable production of these compounds can be realized only through significant improvement of the native production capacity of natural isolates. The most favorable way to achieve this goal is through the genetic manipulation of metabolic pathways that direct the production of these molecules. While random mutagenesis and screening have dominated the industrial production of such compounds in the past our increased understanding of microbial physiology over the last five decades has shifted this trend towards rational approaches for metabolic design. Major drivers of this trend include recombinant DNA technology, high throughput characterization of macromolecular cellular components, quantitative modeling for metabolic engine ring, targeted combinatorial engineering and synthetic biology. In this chapter we track the evolution of microbial engineering technologies from the black box era of random mutagenesis to the science and engineering-driven era of metabolic design.

Directory containing alphabetical and geographic lists of all known alumni of University of North Texas through May 2010. Married women are listed with their maiden names in parentheses.

Fall/spring performances program book from the 2009-2010 school year at the University of North Texas College of Music.

Catalog of courses offered for the 209-2012 school years, outlining general school information, classes, and degree requirements of the University of Texas Southwestern Graduate School of Biomedical Sciences.

Fall/spring performances program book from the 2009-2010 school year at the University of North Texas College of Music.

Fall/spring performances program book from the 2009-2010 school year at the University of North Texas College of Music.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Developing a predictive understanding of subsurface flow and transport is complicated by the disparity of scales across which controlling hydrological properties and processes span. Conventional techniques for characterizing hydrogeological properties (such as pumping, slug, and flowmeter tests) typically rely on borehole access to the subsurface. Because their spatial extent is commonly limited to the vicinity near the wellbores, these methods often can not provide sufficient information to describe key controls on subsurface flow and transport. The field of hydrogeophysics has evolved in recent years to explore the potential that geophysical methods hold for improving the quantification of subsurface properties and processes relevant for hydrological investigations. This chapter is intended to familiarize hydrogeologists and water resource professionals with the state-of-the-art as well as existing challenges associated with hydrogeophysics. We provide a review of the key components of hydrogeophysical studies, which include: geophysical methods commonly used for shallow subsurface characterization; petrophysical relationships used to link the geophysical properties to hydrological properties and state variables; and estimation or inversion methods used to integrate hydrological and geophysical measurements in a consistent manner. We demonstrate the use of these different geophysical methods, petrophysical relationships, and estimation approaches through several field-scale case studies. Among other applications, …

Proceedings of the annual meeting of the Philosophical Society of Texas held on December 3-5, 2010, including a list of attendees, text of addresses, changes to membership, and biographical information about members who have passed.

Volume of the United States Reports containing the final decisions and opinions of the Supreme Court justices regarding cases between April 17 and May 29, 2007. Also includes notes regarding the members of the Supreme Court, orders, and other relevant materials. Index starts on page 1303.

Fall/spring performances program book from the 2009-2010 school year at the University of North Texas College of Music.

Louisiana’s Neutral Strip, an area of pine forests, squats between the Calcasieu and Sabine Rivers on the border of East Texas. Originally a lawless buffer zone between Spain and the United States, its hardy residents formed tight-knit communities for protection and developed a reliance on self, kin, and neighbor. In the early 1900s, the timber boom sliced through the forests and disrupted these dense communities. Mill towns sprang up, and the promise of money lured land speculators, timber workers, unionists, and a host of other characters, such as the outlaw Leather Britches Smith. That moment continues to shape the place’s cultural consciousness, and people today fashion a lore connected to this time. In a fascinating exploration of the region, Keagan LeJeune unveils the legend of Leather Britches, paralleling the stages of the outlaw’s life to the Neutral Strip’s formation. LeJeune retells each stage of Smith’s life: his notorious past, his audacious deeds of robbery and even generosity, his rumored connection to a local union strike—the Grabow War—significant in the annals of labor history, and his eventual death. As the outlaw’s life vividly unfolds, Always for the Underdog also reveals the area’s history and cultural landscape. Often using the particulars of …

Student performances program book from the 2009-2010 school year at the University of North Texas College of Music.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Ledger containing minutes of the City Council in Abilene, Texas documenting the group's discussions and activities from January 14, 2010 to December 16, 2010.

Book of maps of Texas counties depicting major roads, military facilities, and state properties.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

In 1968 James T. Gillam was a poorly focused college student at Ohio University who was dismissed and then drafted into the Army. Unlike most African Americans who entered the Army then, he became a Sergeant and an instructor at the Fort McClellan Alabama School of Infantry. In September 1968 he joined the First Battalion, 22nd Regiment of the 4th Infantry Division in Vietnam. Within a month he transformed from an uncertain sergeant—who tried to avoid combat—to an aggressive soldier, killing his first enemy and planning and executing successful ambushes in the jungle. Gillam was a regular point man and occasional tunnel rat who fought below ground, an arena that few people knew about until after the war ended. By January 1970 he had earned a Combat Infantry Badge and been promoted to Staff Sergeant. Then Washington’s politics and military strategy took his battalion to the border of Cambodia. Search-and-destroy missions became longer and deadlier. From January to May his unit hunted and killed the enemy in a series of intense firefights, some of them in close combat. In those months Gillam was shot twice and struck by shrapnel twice. He became a savage, strangling a soldier in hand-to-hand combat …

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.

Biweekly, comprehensive compilation of decisions, reports, public notices, and other documents of the U.S. Federal Communications Commission.