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A large number of GE techniques can be adapted from other microorganisms to biothreat bacteria and viruses. Detection of GE in a microorganism increases in difficulty as the size of the genetic change decreases. In addition to the size of the engineered change, the consensus genomic sequence of the microorganism can impact the difficulty of detecting an engineered change in genomes that are highly variable from strain to strain. This problem will require comprehensive databases of whole genome sequences for more genetically variable biothreat bacteria and viruses. Preliminary work with microarrays for detecting synthetic elements or virulence genes and analytic bioinformatic approaches for whole genome sequence comparison to detect genetic engineering show promise for attacking this difficult problem but a large amount of future work remains.

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We report a systematic neutron diffraction study on the coexistence of long-range magnetic order and superconductivity in heavy fermion compounds CeRhl-,M,Ins (M=Ir,Co). In addition to the incommensurate antiferromagnetic component in pure CeRhIn5, new type of antiferromagnetic component is found to concur with appearance of superconductivity in the Ir and Co alloy series. There is no detectable effect of the superconducting transition on magnetic order parameters. We compare those results with similar studies we performed on CeRhIn:, under pressure. We also discuss possible theoretical scenarios.

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We have developed a 10-plexed PCR assay coupled to a 12-plexed liquid bead array to rapidly screen environmental samples for B. anthracis, Y. pestis, F. tularensis, and B. melitensis. Highly validated species -specific primer sets were used to simultaneously amplify multiple diagnostic regions unique to each individual pathogen. Resolution of the mix of amplified products was achieved by PCR product hybridization to corresponding probe sequences, attached to unique sets of fluorescent beads. The hybridized beads were processed through a flow cytometer, which detected presence and quantity of each PCR product. The assay was optimized to allow for maximum sensitivity in a multiplexed format. A high- throughput demonstration was performed where 384 simulated environmental samples were spiked with different amounts of B. thuringensis spores and pathogen DNA. The samples were robotically processed to extract DNA and arrayed for multiplexed PCR-liquid bead detection. The assay correctly identified the presence or absence of each pathogen and collected over 3,000 individual data points within a single 8-hour shift for approximately $1.20 per sample in a 10-plexed assay.

Following the Fiscal Year (FY) 2010 (Run-10) Relativistic Heavy Ion Collider (RHIC) Au+Au run, RHIC experiment upgrades sought to improve detector capabilities. In turn, accelerator improvements were made to improve the luminosity available to the experiments for this run (Run-11). These improvements included: a redesign of the stochastic cooling systems for improved reliability; a relocation of 'common' RF cavities to alleviate intensity limits due to beam loading; and an improved usage of feedback systems to control orbit, tune and coupling during energy ramps as well as while colliding at top energy. We present an overview of changes to the Collider and review the performance of the collider with respect to instantaneous and integrated luminosity goals. At the conclusion of the FY 2011 polarized proton run, preparations for heavy ion run proceeded on April 18, with Au+Au collisions continuing through June 28. Our standard operations at 100 GeV/nucleon beam energy was bracketed by two shorter periods of collisions at lower energies (9.8 and 13.5 GeV/nucleon), continuing a previously established program of low and medium energy runs. Table 1 summarizes our history of heavy ion operations at RHIC.

The Lawrence Livermore National Laboratory (LLNL) Chemical & Biological National Security Program (CBNP) provides science, technology, and integrated systems for chemical and biological security. Our approach is to develop and field systems that dramatically improve the nation's capabilities to prevent, prepare for, detect, and respond to terrorist use of chemical or biological weapons.

As shown elsewhere an ablatively imploded shell is hydrodynamically unstable, the dominant instability being the well known Rayleigh-Taylor instability with growth rate {gamma} = {radical}Akg where k = 2{pi}/{lambda} is the wave number, g is the acceleration and A the Attwood number ({rho}{sub hi} {minus} {rho}{sub lo})/({rho}{sub hi} + {rho}{sub lo}) where {rho}{sub hi} is the density of the heavier fluid and {rho}{sub lo} is the density of the lighter fluid. A theoretical understanding of ablative stabilization has gradually evolved, confirmed over the last five years by experiments. The linear growth is very well understood with excellent agreement between experiment and simulation for planar geometry with wavelengths in the region of 30--100{mu}m. There is an accurate, albeit phenomenological dispersion relation. The non-linear growth has been measured and agrees with calculations. In this lecture, the authors go into the fundamentals of the Rayleigh-Taylor instability and the experimental measurements that show it is stabilized sufficiently by ablation in regimes relevant to ICF.

This report describes the advances that have been made over the past two years in testing and characterizing waste material in Hanford tanks.

The status of experimental determination of level structure in odd-odd actinide nuclei is reviewed. A technique for modeling quasiparticle excitation energies and rotational parameters in odd-odd deformed nuclei is applied to actinide species where new experimental data have been obtained by use of neutron-capture gamma-ray spectroscopy. The input parameters required for the calculation are derived from empirical data on single-particle excitations in neighboring odd-mass nuclei. Calculated configuration-specific values for the Gallagher-Moszkowski splittings are used. Calculated and experimental level structures for /sup 238/Np, /sup 244/Am, and /sup 250/Bk are compared, as well as those for several nuclei in the rare-earth region. The agreement for the actinide species is excellent, with bandhead energies deviating 22 keV and rotational parameters 5%, on the average. Applications of this modeling technique are discussed.

The goal of the proposed Spallation Neutron Source (SNS) is to provide a short pulse proton beam of about 0.5{micro}s with average beam power of 1MW. To achieve such purpose, a proton storage ring operated at 60Hz with 1 x 10{sup 14} protons per pulse at 1GeV is required. The Accumulator Ring (AR) receives 1msec long H{sup {minus}} beam bunches of 28mA from a 1GeV linac. Scope and design performance goals of the AR are presented. About 1,200 turns of charge exchange injection is needed to accumulate 1mA in the ring. After a brief description of the lattice design and machine performance parameters, space charge related issues, such as: tune shifts, stopband corrections, halo generation and beam collimation etc. is discussed.

The LOFT Modular Drag Disc Turbine Transducer (MDTT) springs (for range 2, rhoV/sup 2/ = 4900 lbm/ft-sec/sup 2/) were analyzed to determine the static rhoV/sup 2/ load needed to cause a buckling failure. The static load needed to cause elastic buckling was found to be equivalent to a rhoV/sup 2/ value of 431,000 lbm/ft-sec/sup 2/ according to classical buckling theory, but could be as low as rhoV/sup 2/ = 100,000 lbm/ft-sec/sup 2/ due to uncertain end fixity and other spring imperfections.

Parametric instabilities excited by an intense electromagnetic wave in a plasma is a fundamental topic relevant to many applications. These applications include laser fusion, heating of magnetically-confined plasmas, ionospheric modification, and even particle acceleration for high energy physics. In laser fusion, these instabilities have proven to play an essential role in the choice of laser wavelength. Characterization and control of the instabilities is an ongoing priority in laser plasma experiments. Recent progress and some important trends will be discussed. 8 figs.

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This report discusses selected legal issues relating to the authority for summary removal of individuals in senior executive positions at the Department of Veterans Affairs.

The following description and any attachments and references are provided to the Washington State Department of Health (WDOH), Division of Radiation Protection, Air Emissions & Defense Waste Section as a notice of constriction (NOC) in accordance with Washington Administrative Code (WAC) 246-247, Radiation Protection - Air Emissions. The WAC 246-247-060, ''Applications, registration, and licensing'', states ''This section describes the information requirements for approval to construct, modify, and operate an emission unit. Any NOC requires the submittal of information listed in Appendix A.'' Appendix A (WAC 246-247-1 10) lists the requirements that must be addressed. Additionally, the following description, attachments and references are provided to the U.S. Environmental Protection Agency (EPA) as an NOC, in accordance with Title 40 Code of Federal Regulations (CFR), Part 61, ''National Emission Standards for Hazardous Air Pollutants.'' The information required for submittal to the EPA is specified in 40 CFR 61.07. The potential emissions from this activity are estimated to provide less than 0.1 millirem/year total effective dose equivalent (TEDE) to the hypothetical offsite maximally exposed individual (MEI), and commencement is needed within a short time frame. Therefore, this application is also intended to provide notification of the anticipated date of initial startup in accordance …

The possibility of efficient ponderomotive current drive in a magnetized plasma was reported recently in [Phys. Rev. Lett. 91, 205004 (2003)]. Precise limitations on the efficiency are now given through a comprehensive analytical and numerical study of single-particle dynamics under the action of a cyclotron-resonant rf drive in various field configurations. Expressions for the particle energy gain and acceleration along the dc magnetic field are obtained. The fundamental correlation between the two effects is described. A second fundamental quantity, namely the ratio of the potential barrier to the energy gain, can be changed by altering the field configuration. The asymmetric ponderomotive current drive effect can be optimized by minimizing the transverse heating.

As a result of the most recent recalculation one of the indicator parameters, specific conductance, exceeded its background value in downgradient well 299-W19-41, triggering a change from detection monitoring to groundwater quality assessment program. The major contributors to the higher specific conductance are nonhazardous constituents (i.e., sodium, calcium, magnesium, chloride, sulfate, and bicarbonate). Nitrate, chromium, and technetium-99 are present and are increasing; however, they are significantly below their drinking waster standards. Interpretation of groundwater monitoring data indicates that both the nonhazardous constituents causing elevated specific conductance in groundwater and the tank waste constituents present in groundwater at the waste management area are a result of surface water infiltration in the southern portion of the facility. There is evidence for both upgradient and waste management area sources for observed nitrate concentrations. There is no indication of an upgradient source for the observed chromium and technetium-99.

The following report describes a current beam modulation designed to replace the standard regulator and to achieve an ion beam free from amplitude modulation.

The Materials Science and Technology (MST) Handbook was developed by Pacific Northwest National Laboratory, in Richland, Washington, under support from the U.S. Department of Energy. Many individuals have been involved in writing and reviewing materials for this project since it began at Richland High School in 1986, including contributions from educators at the Northwest Regional Education Laboratory, Central Washington University, the University of Washington, teachers from Northwest Schools, and science and education personnel at Pacific Northwest National Laboratory. Support for its development was also provided by the U.S. Department of Education. This introductory course combines the academic disciplines of chemistry, physics, and engineering to create a materials science and technology curriculum. The course covers the fundamentals of ceramics, glass, metals, polymers and composites. Designed to appeal to a broad range of students, the course combines hands-on activities, demonstrations and long term student project descriptions. The basic philosophy of the course is for students to observe, experiment, record, question, seek additional information, and, through creative and insightful thinking, solve problems related to materials science and technology. The MST Teacher Handbook contains a course description, philosophy, student learning objectives, and instructional approach and processes. Science and technology teachers can collaborate to build …

The bibliographic citations in the Experimental Cross Section Information Library (ECSIL) as of July 4, 1976 are tabulated. The tabulation has three arrangements: alphabetically by author, alphabetically by publication, and numerically by reference number.

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After providing collisions of polarized protons at a beam energy of 100 GeV since 2001, the Relativistic Heavy Ion Collider (RHIC) at BNL had its first opportunity to collide polarized protons at its maximum beam energy of 250 GeV in the 2009 polarized proton operations. Equipped with two full Siberian snakes [1] in each ring, RHIC preserves polarization during acceleration from injection to 100 GeV with precise control of the betatron tunes and vertical orbit distortions. However, the strong intrinsic spin resonances beyond 100 GeV are more than two times stronger than those below 100 GeV, requiring much tighter tolerances on vertical orbit distortions and betatron tunes. With the currently achieved orbit correction and tune control, average polarizations of {approx_equal} 42% at top energy and average polarizations of {approx_equal} 55% at injection energy were achieved. Polarization measurements as a function of beam energy also indicated aU polarization losses occurred around three strong intrinsic resonances at 136 GeV, 199.3 GeV and 220.8 GeV Peak luminosity of 122 x 10{sup 30} cm{sup -2} s{sup -1} was also demonstrated. This paper presents the performance of the first RHIC 250 GeV operation and discusses the depolarization issues encountered during the run.